Interpretive search of optimal isocratic and gradient separations in micellar liquid chromatography in extended organic solvent domains

Navarro-Huerta, J.A.; Vargas-Garcia, Armando; Torres-Lapası́o, J.R.; García-Álvarez-Coque, M.C.

doi:10.1016/j.chroma.2019.460784

Cited by 3 publications

(2 citation statements)

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“…Navarro‐Huerta et al. optimized micellar LC (MLC) by isocratic or gradient elution and applied a wide range of models, some of which are developed for RPLC or specifically for MLC [142]. The most accurate predictions were found from the following model with fixed surfactant concentration:

\frac{1}{k} = c_{0} + c_{1} φ + c_{2} φ^{2} + c_{3} φ^{3} + c_{4} \sqrt{φ}

…”

Section: Methodsmentioning

confidence: 99%

Recent applications of retention modelling in liquid chromatography

Uijl

Schoenmakers

Pirok

et al. 2020

J of Separation Science

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Recent applications of retention modelling in liquid chromatography (2015–2020) are comprehensively reviewed. The fundamentals of the field, which date back much longer, are summarized. Retention modeling is used in retention‐mechanism studies, for determining physical parameters, such as lipophilicity, and for various more‐practical purposes, including method development and optimization, method transfer, and stationary‐phase characterization and comparison. The review focusses on the effects of mobile‐phase composition on retention, but other variables and novel models to describe their effects are also considered. The five most‐common models are addressed in detail, i.e. the log‐linear (linear‐solvent‐strength) model, the quadratic model, the log–log (adsorption) model, the mixed‐mode model, and the Neue–Kuss model. Isocratic and gradient‐elution methods are considered for determining model parameters and the evaluation and validation of fitted models is discussed. Strategies in which retention models are applied for developing and optimizing one‐ and two‐dimensional liquid chromatographic separations are discussed. The review culminates in some overall conclusions and several concrete recommendations.

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\frac{1}{k} = c_{0} + c_{1} φ + c_{2} φ^{2} + c_{3} φ^{3} + c_{4} \sqrt{φ}

…”

Section: Methodsmentioning

confidence: 99%

Recent applications of retention modelling in liquid chromatography

Uijl

Schoenmakers

Pirok

et al. 2020

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…A comparison study that uses both Tween 20 and Tween 40 (Figure 2) as the only isocratic MP modifiers above their CMC is presented in this paper. Although gradient elution studies using an organic solvent with MLC have been reported [50,51], an important aspect of our study was to avoid the use of any organic modifier solvents in the mobile phase and to maintain the inherent "green" advantage of MLC [52]. The determination of HCA derivatives is of crucial importance due to its availability in food, beverages, and medications.…”

Section: Introductionmentioning

confidence: 99%

Ultra-High-Performance Micellar Liquid Chromatography Comparing Tween 20 and Tween 40 for the Determination of Hydroxycinnamic Acids

Ali

Danielson

2022

Separations

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A simple and green ultra-high-performance micellar liquid chromatography (MLC) method was developed here, comparing Tween 20 and Tween 40 for the first time as the only mobile phase modifiers with a C18 column. Its application to the separation of nine hydroxycinnamic acid (HCA) derivatives (cinnamic, caffeic, ferulic, sinapic, o-, m-, p-coumaric, 3,4-dihydroxyhydrocinnamic, and chlorogenic acids) was made, due to their importance as antioxidants in a variety of natural beverages such as wine and coffee. The optimal conditions of 45 °C temperature (T), 1% surfactant in the mobile phase, and pH control with 2.5 mM sulfuric acid were determined and used to elucidate the analytical figures of merit. Although the effect of these conditions was insignificant between the two surfactants, the nine-component HCA mixture was separated faster—in about 15 min—and with less peak tailing using Tween 20 than with Tween 40. The linearity of the Van’t Hoff (lnk versus 1/T) plots was evident for Tween 20, indicating a single retention mechanism—but less so for Tween 40. The equilibrium constants of the analytes with the micelles and the stationary phase were calculated. The developed method was successfully used to analyze organic red wine, spiked organic red wine, and green coffee diet pills. The percent recoveries of the nine HCA compounds spiked in the organic red wine ranged from 90% to 107%. The green coffee extract diet pills showed the presence of a significant amount of chlorogenic acid.

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