Interpretation of the optical transfer function: Significance for image scanning microscopy

Sheppard, Colin J. R.; Roth, Stephan; Heintzmann, Rainer; Castello, Marco; Vicidomini, Giuseppe; Chen, Rui; Chen, Xudong; Diaspro, Alberto

doi:10.1364/oe.24.027280

Cited by 30 publications

(14 citation statements)

References 19 publications

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“…In practice, we choose a detector size as a compromise between obtaining optical sectioning and detecting an adequate signal. In order to take account of this reduction in signal, it is useful not to normalize the OTF, but to retain the value of C (0) as equal to the strength of the signal [66].…”

Section: Image Formation In Image Scanning Microscopymentioning

confidence: 99%

Structured illumination microscopy and image scanning microscopy: a review and comparison of imaging properties

Sheppard

2021

Phil. Trans. R. Soc. A.

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Structured illumination microscopy and image scanning microscopy are two microscopical tech- niques, rapidly increasing in practical application, that can result in improvement in transverse spatial resolution, and/or improvement in axial imaging performance. The history and principles of these techniques are reviewed, and the imaging properties of the two methods compared. This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 1)’.

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Section: Image Formation In Image Scanning Microscopymentioning

confidence: 99%

Structured illumination microscopy and image scanning microscopy: a review and comparison of imaging properties

Sheppard

2021

Phil. Trans. R. Soc. A.

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“…It can be found from Eq. (2) that once the optical configuration of the microscope is fixed (the pupil function of the objective lens is pre-defined), the PTF is fully determined by the illumination function [39]. In order to reconstruct the sample's quantitative phase information from Eq.…”

Section: Derivation Of Optimal Illumination Schemementioning

confidence: 99%

Optimal illumination scheme for isotropic quantitative differential phase contrast microscopy

et al. 2019

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Differential phase contrast microscopy (DPC) provides high-resolution quantitative phase distribution of thin transparent samples under multi-axis asymmetric illuminations. Typically, illumination in DPC microscopic systems is designed with 2-axis half-circle amplitude patterns, which, however, result in a non-isotropic phase contrast transfer function (PTF). Efforts have been made to achieve isotropic DPC by replacing the conventional half-circle illumination aperture with radially asymmetric patterns with 3-axis illumination or gradient amplitude patterns with 2-axis illumination. Nevertheless, these illumination apertures were empirically designed based on empirical criteria related to the shape of the PTF, leaving the underlying theoretical mechanisms unexplored. Furthermore, the frequency responses of the PTFs under these engineered illuminations have not been fully optimized, leading to suboptimal phase contrast and signal-to-noise ratio (SNR) for phase reconstruction. In this Letter, we provide a rigorous theoretical analysis about the necessary and sufficient conditions for DPC to achieve perfectly isotropic PTF. In addition, we derive the optimal illumination scheme to maximize the frequency response for both low and high frequencies (from 0 to 2N A ob j ), and meanwhile achieve perfectly isotropic PTF with only 2-axis intensity measurements. We present the derivation, implementation, simulation and experimental results demonstrating the superiority of our method over state-of-the-arts in both phase reconstruction accuracy and noise-robustness.

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“…The optimization of system parameters and the evaluation of imaging quality are the focuses of research in holographic stereogram printing, and the optical transfer function (OTF) is an efficient tool to evaluate the imaging quality of holographic stereogram from the aspect of frequency response 38 . St.-Hilaire constructed the modulation transfer function (MTF) of horizontal-parallax-only (HPO) image-plane holographic stereograms, and discussed the optimum sampling of the slit plane with fixed depth object points 39 – 41 .…”

Section: Introductionmentioning

confidence: 99%

Characteristic and optimization of the effective perspective images’ segmentation and mosaicking (EPISM) based holographic stereogram: an optical transfer function approach

Su¹,

Yan²,

Jiang³

et al. 2018

Sci Rep

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Based on our proposed method for holographic stereogram printing using effective perspective images’ segmentation and mosaicking (EPISM), we analyze the reconstructed wavefront errors, and establish the exit pupil function model of proposed printing system. To evaluate the imaging quality, the optical transfer function (OTF) of the holographic stereogram is modelled from the aspect of frequency response. The characteristic of the OTF with respect to the exit pupil size and the aberration are investigated in detail. We also consider the flipping effect in spatial domain. The optimization of hogel sizes, i.e., the sampling interval of original perspective images and the printing interval of synthetic effective perspective images, are given for the optimized reconstruction. Numerical simulations and optical experiments are implemented, and the results demonstrate the validity of our analysis, and the optimized parameters of hogel sizes can improve the imaging quality of full parallax holographic stereogram effectively.

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Interpretation of the optical transfer function: Significance for image scanning microscopy

Cited by 30 publications

References 19 publications

Structured illumination microscopy and image scanning microscopy: a review and comparison of imaging properties

Structured illumination microscopy and image scanning microscopy: a review and comparison of imaging properties

Optimal illumination scheme for isotropic quantitative differential phase contrast microscopy

Characteristic and optimization of the effective perspective images’ segmentation and mosaicking (EPISM) based holographic stereogram: an optical transfer function approach

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