INTERPRETATION OF ISOLATED HIV ANTI-p24 REACTIVITY IN WESTERN BLOT ANALYSIS

Lelie, P. N.; Poel, C.L. van der; Reesink, H. W.

doi:10.1016/s0140-6736(87)90274-1

Cited by 29 publications

(9 citation statements)

References 3 publications

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“…or more commonly, p24/p55, have been described previously (18)(19)(20)(21)(22). For this reason, we graded the serologic responses to p24 and did not consider positive any SS serum that scored <2+ on a semiquantitative scale.…”

Section: Resultsmentioning

confidence: 96%

Detection of serum antibodies to retroviral proteins in patients with primary sjögren's syndrome (autoimmune exocrinopathy)

Talal

Dauphinée

Dang

et al. 1990

Arthritis & Rheumatism

215

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Primary Sjögren's syndrome (SS) is considered a benign autoimmune disease; it is characterized by lymphoid infiltration of salivary and lacrimal glands, often accompanied by the presence of serum autoantibodies, particularly anti‐Ro (SS‐A) and anti‐La (SS‐B). There are important immunologic similarities between primary SS and acquired immunodeficiency syndrome. To investigate for a possible immune response to retroviral proteins in primary SS, we performed immunoblotting against human immunodeficiency virus‐1 (HIV‐1) proteins using sera from 47 patients with primary SS. Moderate‐to‐strong reactivity, suggesting the presence of serum antibodies, was found in 14 patients (30%). Of 120 normal subjects, only 1 showed moderate positivity. All 14 positive SS sera reacted against p24 (gag) but failed to react against gp41 or gp120 (env). This response did not reflect hypergammaglobulinemia since immunoglobulin concentrations among the 29 SS patients studied were the same in sera that contained and sera that did not contain anti‐gag reactivity. Two sera also reacted against p17 gag. Four reacted against HIV‐2 core proteins, but none reacted with core proteins of human T lymphotropic virus‐I. Only 1 of the 14 sera reacted against Ro (SS‐A), and 1 other reacted against La (SS‐B). These results identify a subset of SS patients characterized by 1) the presence of serum antibodies to HIV‐1 group‐specific, but not type‐specific, proteins, and 2) the relative absence of anti‐Ro (SS‐A) and anti‐La (SS‐B) autoantibodies. In this latter respect, these SS patients constitute a subpopulation that resembles patients with HIV‐induced SS‐like disease.

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Section: Resultsmentioning

confidence: 96%

Detection of serum antibodies to retroviral proteins in patients with primary sjögren's syndrome (autoimmune exocrinopathy)

Talal

Dauphinée

Dang

et al. 1990

Arthritis & Rheumatism

215

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“…False-negative results for env-specific antibodies are possible. There are several reports (11,19,20) which demonstrate that the HIV immunoblot technique does not detect env-specific antibodies as readily as gag-specific antibodies in the early stages of HIV seroconversion. One report (19) shows that reproducibility of detection of gpl2Oenv antibodies by immunoblot is affected significantly by variations in the procedures used for preparation of the immunoblot strips.…”

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confidence: 99%

Antibodies reactive with human immunodeficiency virus gag-coded antigens (gag reactive only) are a major cause of enzyme-linked immunosorbent assay reactivity in a blood donor population

et al. 1988

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Normal blood donors were examined for human immunodeficiency virus (HIV)-reactive antibodies with both virus-and Escherichia coli-expressed env-and gag-coded antigens. The frequency of samples from normal (low-risk) donors that were repeatedly reactive with an HIV enzyme-linked immunosorbent assay blood screening test (Du Pont Co.) was 0.6%. Two classes of HIV serological reactivity were identified: a minor env-reactive class (0.03 to 0.06% of donors) and the predominant env-nonreactive gag-reactive class (gag reactive only [GRO]) (0.4 to 0.5% of donors). Assignment of env reactivity was made by a synthetic (recombinant) env enzyme-linked immunosorbent assay and virus immunoblot. Most GRO sera reacted with pl5/pl7 bands on HIV immunoblot. Antibody specificity in GRO sera was confirmed by competition-binding studies with viral gag and E. coli-expressed p55gac. This study provides independent verification that gag-specific antibodies are present in many env-nonreactive sera. More serological and virological studies of individuals with this antibody pattern should be pursued to determine the origin of these gag-reactive antibodies.

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“…Western blot was less sensitive for HIV antibody recognition than the synthetic peptide assay (Behring) and antibodies to core were always the first to be recognized. Many reports pointed to the unspecific nature of this initial antibody pattern in the Western blot assay [5,6], First-generation assays were the least sensitive tests for recognition of HIV antibody response. In accordance with these data we suggest that ELISA using synthetic peptides seems the best approach to the use of blood banks in order to detect HIV seroconversion as early as possible.…”

mentioning

confidence: 99%