“…After staining, slides were evaluated by two pathologists, independently. After evaluation of 5 non-necrosis representative fields separated over the tumor tissue, the staining intensity score (negative, weak, moderate, and strong scored as 0, 1, 2, and 3, respectively) was multiplied by score of positively stained cells (0, none; 1, 1–40%; and 2, 40–70%; 3, 70–100%) to obtain the overall score (Bian et al, 2015).…”