Interpretation of fish biomarker data for identification, classification, risk assessment and testing of endocrine disrupting chemicals

Dang, ZhiChao

doi:10.1016/j.envint.2016.04.003

Cited by 39 publications

(20 citation statements)

References 161 publications

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“…However, induction of VTG does not provide conclusive evidence on the mechanism of action of the identified EDC. It has been well established that ER activation induces VTG production, but recent studies have shown that AR, PR and GR activation may result in altered VTG levels as well (Dang 2016).…”

Section: Figurementioning

confidence: 99%

“…The concentration of vitellogenin (VTG) is the most widely used biomarker for EDC activity, in particular for compounds that activate ER (Dang 2016). VTG is an egg yolk precursor protein, which is produced and secreted by the liver, taken up by the ovary and modified by developing eggs to form the egg yolk.…”

Section: Figurementioning

confidence: 99%

“…The zebrafish is widely used as a model organism to identify EDCs, and to perform risk assessment for both humans and wildlife (Segner 2009, Dang 2016. Research focuses on the development of screening assays for the activity of EDCs and their mechanism of action.…”

Section: The Zebrafish Estrogen Receptors (Ers)mentioning

confidence: 99%

“…Phenotypic endpoints like growth, sexual differentiation, sex ratio, egg production and fertilization success are commonly used for monitoring EDC activity, and these biomarkers are used in partial and full life-cycle studies (Segner 2009, Dang 2016). Partial life-cycle studies may involve investigating the impact of short-term exposure in mature actively reproducing individuals, and in these studies, endpoints like fertilization success and fecundity are measured, which may be complemented by gonad histopathology or plasma levels of sex steroids R70 Review m j m schaaf Zebrafish nuclear receptors 59 1 : R70 Review or VTG.…”

Section: Figurementioning

confidence: 99%

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Nuclear receptor research in zebrafish

Schaaf

2017

Journal of Molecular Endocrinology

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Nuclear receptors (NRs) form a superfamily of transcription factors that can be activated by ligands and are involved in a wide range of physiological processes. NRs are well conserved between vertebrate species. The zebrafish, an increasingly popular animal model system, contains a total of 73 NR genes, and orthologues of almost all human NRs are present. In this review article, an overview is presented of NR research in which the zebrafish has been used as a model. Research is described on the three most studied zebrafish NRs: the estrogen receptors (ERs), retinoic acid receptors (RARs) and peroxisome proliferator-activated receptors (PPARs). The studies on these receptors illustrate the versatility of the zebrafish as a model for ecotoxicological, developmental and biomedical research. Although the use of the zebrafish in NR research is still relatively limited, it is expected that in the next decade the full potential of this animal model will be exploited.

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Section: Figurementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: The Zebrafish Estrogen Receptors (Ers)mentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

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Nuclear receptor research in zebrafish

Schaaf

2017

Journal of Molecular Endocrinology

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“…Zebrafish are commonly used for monitoring EDC activity. Phenotypic endpoints like growth, sexual differentiation, sex ratio, egg production and fertilization success are commonly used for monitoring EDC activity, and these biomarkers are used in partial and full life cycle studies [12,13]. A lot of studies on zebrafish steroid receptor action have aimed to identify novel molecular biomarkers or screening approaches for EDC activity which could replace or complement the phenotypic endpoints.…”

Section: Steroid Receptor Research In Zebrafishmentioning

confidence: 99%

The First Fifteen Years of Steroid Receptor Research in Zebrafish; Characterization and Functional Analysis of the Receptors

Schaaf¹

2017

Nuclear Receptor Research

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Abstract. Steroid hormones regulate a wide range of processes in our body, and their effects are mediated by steroid receptors. In addition to their physiological role, these receptors mediate the effects of endocrine disrupting chemicals (EDCs) and are widely used targets for dugs involved in the treatment of numerous diseases, ranging from cancer to inflammatory disorders. Over the last fifteen years, the zebrafish has increasingly been used as an animal model in steroid receptor research. Orthologues of all human steroid receptor genes appear to be present in zebrafish. All zebrafish steroid receptors have been characterized in detail, and their expression patterns have been analyzed. Functional studies have been performed using morpholino knockdown of receptor expression and zebrafish lines carrying mutations in one of their steroid receptor genes. To investigate the activity of the receptors in vivo, specific zebrafish reporter lines have been developed, and transcriptomic studies have been carried out to identify biomarkers for steroid receptor action. In this review, an overview of research on steroid receptors in zebrafish is presented, and it is concluded that further exploitation of the possibilities of the zebrafish model system will contribute significantly to the advancement of steroid receptor research in the next decade.

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Use of All‐Male cyp17a1‐Deficient Zebrafish (Danio rerio) for Evaluation of Environmental Estrogens

Jia,

Shi,

Liu

et al. 2024

Enviro Toxic and Chemistry

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Natural and synthetic environmental estrogens (EEs) are widespread and have received extensive attention. Our previous studies demonstrated that depletion of the cytochrome P450 17a1 gene (cyp17a1) leads to all‐testis differentiation phenotype in zebrafish and common carp. In the present study, cyp17a1‐deficient zebrafish with defective estrogen biosynthesis were used for the evaluation of EEs, as assessed by monitoring vitellogenin (vtg) expression. A rapid and sensitive assessment procedure was established with the 3‐day administration of estradiol (E2), followed by examination of the transcriptional expression of vtgs in our cyp17a1‐deficient fish. Compared with the control fish, a higher E2‐mediated vtg upregulation observed in cyp17a1‐deficient zebrafish exposed to 0.1 μg/L E2 is known to be estrogen receptor–dependent and likely due to impaired in vivo estrogen biosynthesis. The more responsive vtg expression in cyp17a1‐deficient zebrafish was observed when exposed to 200 and 2000 μg/L bisphenol A (BPA) and perfluoro‐1‐octanesulfonate (PFOS). The estrogenic potentials of E2, BPA, and PFOS were compared and assessed by the feminization effect on ovarian differentiation in cyp17a1‐deficient zebrafish from 18 to 50 days postfertilization, based on which a higher sensitivity of E2 in ovarian differentiation than BPA and PFOS was concluded. Collectively, through the higher sensitivity to EEs and the capacity to distinguish chemicals with different estrogenic potentials exhibited by the all‐male cyp17a1‐deficient zebrafish with impaired estrogen biosynthesis, we demonstrated that they can be used as an excellent in vivo model for the evaluation of EEs. Environ Toxicol Chem 2024;00:1–13. © 2024 SETAC

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Interpretation of fish biomarker data for identification, classification, risk assessment and testing of endocrine disrupting chemicals

Cited by 39 publications

References 161 publications

Nuclear receptor research in zebrafish

Nuclear receptor research in zebrafish

The First Fifteen Years of Steroid Receptor Research in Zebrafish; Characterization and Functional Analysis of the Receptors

Use of All‐Male cyp17a1‐Deficient Zebrafish (Danio rerio) for Evaluation of Environmental Estrogens

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