Interpretation of bovine serum total calcium: effects of adjustment for albumin and total protein

Seifi, Hesam A; Mohri, Mehrdad; Ehsani, A.; Hosseini, Ebrahim; Chamsaz, Mahmoud

doi:10.1007/s00580-005-0582-2

Cited by 19 publications

(27 citation statements)

References 4 publications

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“…Moreover, a fraction of blood calcium is in protein-bound form, principally bound to albumin, whereas ionized calcium is the biologically active fraction. 43,44 In the present study, although the total calcium concentrations were seemingly less than the reference limit on day 10, the adjusted calcium concentrations were within reference limits. 43 Reduced albumin concentrations in the periparturient period might contribute to the ability of the cows to maintain higher concentrations of ionized calcium, despite the reduced total serum calcium concentration.…”

Section: Discussioncontrasting

confidence: 50%

“…41 We detected only a weak correlation between calcium concentrations and MGSH values in moderately conditioned and overconditioned cows in the postpartum period. 43 Reduced albumin concentrations in the periparturient period might contribute to the ability of the cows to maintain higher concentrations of ionized calcium, despite the reduced total serum calcium concentration. 42 Albumin-associated adjustments were made in total calcium concentrations to reflect this fact.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the correlation between serum biochemical values and liver ultrasonographic indices in periparturient cows with different body condition scores

Rafia

Taghipour-Bazargani²,

Asadi³

et al. 2012

ajvr

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Section: Discussioncontrasting

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Evaluation of the correlation between serum biochemical values and liver ultrasonographic indices in periparturient cows with different body condition scores

Rafia

Taghipour-Bazargani²,

Asadi³

et al. 2012

ajvr

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“…It has been hypothesized that blood iCa concentration more accurately reflects the functional Ca status of the cow because this is the form of Ca readily available to cells to perform intracellular signaling necessary for such functions as contraction of muscles and activation of immune cells (Kimura et al, 2006); in addition, tCa is affected by blood protein levels (Rosol et al, 1995;Seifi et al, 2005). Ionized Ca is challenging to accurately measure because exposure of samples to air as well as the use of anticoagulants can change the proportion of Ca that is ionized in the sample (Boink et al, 1991).…”

mentioning

confidence: 99%

Short communication: Relationship between methods for measurement of serum electrolytes and the relationship between ionized and total calcium and neutrophil oxidative burst activity in early postpartum dairy cows

Leno

Martens

Felippe

et al. 2017

Journal of Dairy Science

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The objectives of this study were to (1) compare a test for serum measurement of total Ca (tCa), Mg, and P (VetTest Chemistry Analyzer, IDEXX Laboratories Inc., Westbrook, ME) to reference methods (spectrophotometric assays on a Beckman Coulter 640e automated clinical chemistry analyzer; Beckman Coulter, Brea, CA), (2) determine the relationship between ionized Ca (iCa) and reference method tCa in the immediate postpartum period, and (3) assess the relative value of these blood Ca indices as predictors of neutrophil oxidative burst activity. Samples were collected from multiparous Holstein cows (n = 33) over the first 5 d in milk. A total of 183 samples for objective 1 and 181 samples for objective 2 were available. Neutrophil oxidative burst activity was assessed once between 2 and 5 d in milk (n = 29). Linear regression demonstrated strong relationships between serum tCa, Mg, and P concentrations measured by the VetTest compared with the reference method. Bland Altman analysis indicated that the VetTest values were higher than the reference method by 0.22 mmol/L for tCa, 0.12 mmol/L for Mg, and 0.16 mmol/L for P. Compared with hypocalcemia categorized at ≤2.0 or ≤2.125 mmol/L with the reference method tCa, thresholds for the VetTest measured tCa of ≤2.23 mmol/L (sensitivity = 87%, specificity = 89%) or ≤2.30 mmol/L (sensitivity = 86%, specificity = 96%) could be used. The relationship between whole-blood iCa and reference method serum tCa differed by sampling time point after calving. Compared with identification of hypocalcemia with serum tCa measurements from the reference method (thresholds of ≤2.0 and 2.125 mmol/L), a whole-blood iCa threshold of ≤1.17 mmol/L resulted in the highest combined sensitivities (94 and 82%) and specificities (80 and 94%) at either threshold. Ionized Ca measurements were more consistently related to outcomes of neutrophil oxidative burst activity measured in vitro. The VetTest measurements of serum tCa reliably identified hypocalcemia when thresholds were adjusted to account for the bias of the test. The variation in the relationship between iCa and reference method tCa in the days following parturition suggest that these measures cannot be used interchangeably as indicators of Ca status. The more consistent associations between iCa and in vitro measures of neutrophil function, compared with tCa, indicated that this may be a more sensitive predictor of functional outcomes associated with postpartum Ca status.

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“…Embora sejam condições diferentes, algumas possíveis explicações para a hipoproteinemia são: redução da síntese proteica hepática e/ou aumento da taxa de catabolismo proteico, diluição da albumina no soro pelo aumento da volemia ou extravasamento de proteína para o espaço intersticial. Outra possível explicação é o desvio das proteí-nas circulantes em resposta a alta demanda para a síntese de leite, em vacas em início de lactação (Goff 2000, Seifi et al 2005.…”

Section: Resultsunclassified

Hipocalcemia no deslocamento de abomaso de bovinos: estudo de 39 casos

et al. 2017

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RESUMO: Este estudo avaliou o status do cálcio sérico em 39 vacas com deslocamento de abomaso (DA), provenientes de 30 propriedades leiteiras selecionadas, na região de Campos Gerais no Paraná. O diagnóstico do deslocamento de abomaso foi realizado por percussão auscultatória, além dos sinais clínicos e informações da anamnese. Previamente ao procedimento cirúrgico, amostras de sangue foram coletadas para a mensuração dos níveis sanguíneos de cálcio, albumina, proteína e glicose. Como grupo controle, amostras de sangue foram coletadas de vacas hígidas que se encontravam em semelhante período de lactação. Das 39 vacas com deslocamento de abomaso, 35 apresentaram hipocalcemia e no grupo controle, apenas um animal. Concentrações sanguíneas de glicose e proteína foram inferiores nos animais com DA, quando comparados com animais do grupo controle.

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Interpretation of bovine serum total calcium: effects of adjustment for albumin and total protein

Cited by 19 publications

References 4 publications

Evaluation of the correlation between serum biochemical values and liver ultrasonographic indices in periparturient cows with different body condition scores

Evaluation of the correlation between serum biochemical values and liver ultrasonographic indices in periparturient cows with different body condition scores

Short communication: Relationship between methods for measurement of serum electrolytes and the relationship between ionized and total calcium and neutrophil oxidative burst activity in early postpartum dairy cows

Hipocalcemia no deslocamento de abomaso de bovinos: estudo de 39 casos

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