Interpopulation Crosses, Inheritance Study, and Genetic Variability in the Brown Planthopper Complex, Nilaparvata lugens (Homoptera: Delphacidae)

Latif, Abdul; Omar, M. Y.; Tan, Soon Guan; Siraj, Siti Shapor; Ismail, Aziah

doi:10.1007/s10528-009-9316-5

Cited by 11 publications

(8 citation statements)

References 28 publications

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“…In addition to the apparent genetic differences between insect biotypes, several indications of associations between secondary symbionts and biotype populations in insects, particularly whitefly and aphid, have been reported (4,37). Previous studies have detected biological differences among N. lugens biotype populations (6,25,36). The three biotypes of N. lugens were maintained on specific rice varieties for a long time.…”

Section: Discussionmentioning

confidence: 99%

“…In N. lugens, the biotype is assigned to a population with the ability to damage varieties of rice that carry resistance genes and that were previously resistant to it (5). It has been claimed that some biotypes of N. lugens differ in small morphological features, isozymes, and DNA polymorphisms (6,25,36). However, the precise nature of the virulence-conferring mechanisms in N. lugens biotypes (and their modes and stability of inheritance) is not clear.…”

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confidence: 99%

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Bacterial Symbionts of the Brown Planthopper,Nilaparvata lugens(Homoptera: Delphacidae)

Tang

Jing

et al. 2010

Appl Environ Microbiol

115

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The brown planthopper (Nilaparvata lugens Stål), the most destructive pest of rice, has been identified, including biotypes with high virulence towards previously resistant rice varieties. There have also been many reports of a yeast-like symbiont of N. lugens, but little is known about the bacterial microbes. In this study, we examined the bacterial microbes in N. lugens and identified a total of 18 operational taxonomic units (OTUs) representing four phyla (Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes) by sequencing and analyzing 16S rRNA gene libraries obtained from three populations of N. lugens, which were maintained on the rice varieties TN1, Mudgo, and ASD7. Several of the OTUs were similar to previously reported secondary symbionts of other insects, including an endosymbiont of the psyllid Glycapsis brimblecombei, an Asaia sp. found in the mosquito Anopheles stephensi, and Wolbachia, found in the mite Metaseiulus occidentalis. However, the species and numbers of the detected OTUs differed substantially among the N. lugens populations. Further, in situ hybridization analysis using digoxigenin-labeled probes indicated that OTU 1 was located in hypogastrium tissues near the ovipositor and ovary in biotype 1 insects, while OTU 2 was located in the front of the ovipositor sheath in biotype 2 insects. In addition, masses of bacterium-like organisms were observed in the tubes of salivary sheaths in rice plant tissues that the insects had fed upon. The results provide indications of the diversity of the bacterial microbes harbored by the brown planthopper and of possible associations between specific bacterial microbes and biotypes of N. lugens.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Bacterial Symbionts of the Brown Planthopper,Nilaparvata lugens(Homoptera: Delphacidae)

Tang

Jing

et al. 2010

Appl Environ Microbiol

115

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“…The analyses of isozymes and RAPD-PCR markers indicated that BPH captured from rice plant has high esterase activity compared to BPH captured from L. hexandra and they represent two distinct closely . related sibling species in Malaysia [14,15]. Latif et al [16] reported that Direct amplified length polymorphism (DALP) also useful for the analysis of genetic polymorphisms in brown plant hopper, N. lugens populations.…”

Section: Introductionmentioning

confidence: 99%

Genetic Structure and Phylogenetic Status of Rice Brown Plant Hopper (BPH), Nilaparvata lugens Isolated from Kerala, India

2015

J App Biol Biotech

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Brown plant hopper, Nilaparvata lugens is a major pest of paddy, which damages the plants directly by sucking the sap and indirectly by transmission of rice viruses such as ragged stunt virus and grassy stunt virus. Geographically N. lugens are distributed throughout South and South East Asia where rice is grown and feed the phloem of plant. Due to the uncontrolled use of insecticides, insecticide resistant plant hoppers were emerged in many geographical locations like Japan, South and Southeast Asia etc. In this study we have described the Phylogenetic status of N. lugens isolated from Kerala, India. This study revealed that, N. lugens isolated from Andhra Pradesh and a few populations of N. lugens isolated from China and Japan were genetically similar to N. lugens isolated from Kerala. The result of this study also revealed the biasness of COI sequence of N. lugens to nucleotides AT.

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“…Based on nymphal survival, virulence, ovipositional preference, mate choice, and hybridization experiments, Claridge et al [5] suggested that the rice and Leersia infesting populations of brown planthopper (BPH) represented two distinct sympatric biological species. In recent studies, the analyses of isozymes and RAPD-PCR markers indicated that BPH with high esterase activity usually captured from rice plant, and those with low esterase activity usually captured from L. hexandra in Malaysia represent two distinct closely related sibling species [6, 7]. …”

Section: Introductionmentioning

confidence: 99%

Genetic Dissection of Sympatric Populations of Brown Planthopper,Nilaparvata lugens(Stål), Using DALP-PCR Molecular Markers

Latif

Rafii

Mazid

et al. 2012

The Scientific World Journal

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Direct amplified length polymorphism (DALP) combines the advantages of a high-resolution fingerprint method and also characterizing the genetic polymorphisms. This molecular method was also found to be useful in brown planthopper, Nilaparvata lugens species complex for the analysis of genetic polymorphisms. A total of 11 populations of Nilaparvata spp. were collected from 6 locations from Malaysia. Two sympatric populations of brown planthopper, N. lugens, one from rice and the other from a weed grass (Leersia hexandra), were collected from each of five locations. N. bakeri was used as an out group. Three oligonucleotide primer pairs, DALP231/DALPR′5, DALP234/DALPR′5, and DALP235/DALPR′5 were applied in this study. The unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on genetic distances for the 11 populations of Nilaparvata spp. revealed that populations belonging to the same species and the same host type clustered together irrespective of their geographical localities of capture. The populations of N. lugens formed into two distinct clusters, one was insects with high esterase activities usually captured from rice and the other was with low esterase activities usually captured from L. hexandra. N. bakeri, an out group, was the most isolated group. Analyses of principal components, molecular variance, and robustness also supported greatly to the findings of cluster analysis.

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Interpopulation Crosses, Inheritance Study, and Genetic Variability in the Brown Planthopper Complex, Nilaparvata lugens (Homoptera: Delphacidae)

Cited by 11 publications

References 28 publications

Bacterial Symbionts of the Brown Planthopper,Nilaparvata lugens(Homoptera: Delphacidae)

Bacterial Symbionts of the Brown Planthopper,Nilaparvata lugens(Homoptera: Delphacidae)

Genetic Structure and Phylogenetic Status of Rice Brown Plant Hopper (BPH), Nilaparvata lugens Isolated from Kerala, India

Genetic Dissection of Sympatric Populations of Brown Planthopper,Nilaparvata lugens(Stål), Using DALP-PCR Molecular Markers

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