“…Mouse monoclonal antibodies included: anti-GFP (1:400, MP Biomedicals), anti-Oct4 (1:200, Santa Cruz Biotechnology), anti-nestin (1:200, Upstate), anti-Tuj1 (1:500, Sigma), anti-MAP2 (1:500, Sigma), anti-AP2γ (1:500, Abcam), anti-cytokeratin18 (1:200, Abcam) and anti-cytokeratin14 (1:50, Abcam). Rabbit polyclonal antibodies were anti-GFP (1:400, Santa Cruz Biotechnology), an anti-Sox1/(2)/3 (1:100) that has a preference for Sox1 and Sox3 over Sox2 [78,79]. Fluorescein isothiocyanate-and Cy3-conjugated secondary antibodies were used (1:200 and 1:500, Jackson ImmunoResearch).…”