Interplay of ROS and behavioral pattern in fluoride exposed Drosophila melanogaster

Khatun, Salma; Mandi, Moutushi; Rajak, Prem; Roy, Sumedha

doi:10.1016/j.chemosphere.2018.06.074

Cited by 36 publications

(19 citation statements)

References 71 publications

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“…The significant reduction in lifespan of fluoride treated flies was similar to the findings of Khatun et al (2018), where NaF exposure to D. melanogaster in the parental generation led to an increase in adult mortality. Significant reduction in the climbing activity was also seen in the flies treated with NaF.…”

Section: Discussionsupporting

confidence: 82%

“…Significant reduction in the climbing activity was also seen in the flies treated with NaF. Similarly, Khatun et al (2018) and Sarkar, Roy, and Roy (2018) also observed alteration in climbing behaviour in flies exposed to sub lethal concentrations of fluoride. Fluoride is thought to inhibit the activity of antioxidant enzymes, such as superoxide dismutase, glutathione peroxidase and catalase.…”

Section: Discussionmentioning

confidence: 57%

“…Drosophila melanogaster is an acceptable model for understanding the human condition under stress of toxicants because of the abundance of highly conserved genes and pathways controlling development, stress response and xenobiotic metabolism across these two divergent species (Mackay & Anholt, 2006;Misra, Horner, Lam, & Thummel, 2011;Sykiotis & Bohmann, 2010). A number of studies have been conducted on Drosophila model using priority environmental contaminants and toxicants including mercury (Rand, Dao, & Clason, 2009), lead (Hirsch et al, 2003), arsenic (Ortiz, Opoka, Kane, & Cartwright, 2009), fluoride (Dutta, Rajak, Khatun, & Roy, 2017;Khatun, Mandi, Rajak, & Roy, 2018), manganese (Bonilla et al, 2012), ethanol (Guarnieri & Heberlein, 2003), nanoparticles (Posgai et al, 2011), pesticides (Gupta et al, 2007;Das, Podder, & Roy, 2010;Chmiel, Daisley, Burton, & Reid, 2019), nanopesticides (Demir, 2020) and solvents (Wasserkort & Koller, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Effects of sodium fluoride and Ocimum sanctum extract on the lifespan and climbing ability of Drosophila melanogaster

Perveen

Kumari

Raj

et al. 2021

JoBAZ

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Background Fluoride may induce oxidative stress and apoptosis. It may also lead to neurobehavioural defects including neuromuscular damage. The present study aimed to explore the effects of sub lethal concentrations of sodium fluoride (NaF) on the lifespan and climbing ability of Drosophila melanogaster. In total, 0.6 mg/L and 0.8 mg/L of NaF were selected as sublethal concentrations of NaF for the study. Lifespan was measured and climbing activity assay was performed. Results The study showed significant decrease in lifespan of flies treated with fluoride. With increasing age, significant reduction in climbing activity was observed in flies treated with sodium fluoride as compared to normal (control) flies. Flies treated with tulsi (Ocimum sanctum) and NaF showed increase in lifespan and climbing activity as compared to those treated with NaF only. Lipid peroxidation assay showed significant increase in malondialdehyde (MDA) values in the flies treated with NaF as compared to control. The MDA values decreased significantly in flies treated with tulsi mixed with NaF. Conclusions The results indicate that exposure to sub lethal concentration of NaF may cause oxidative stress and affect the lifespan and climbing activity of D. melanogaster. Tulsi extract may help in reducing the impact of oxidative stress and toxicity caused by NaF.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 57%

Section: Introductionmentioning

confidence: 99%

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Effects of sodium fluoride and Ocimum sanctum extract on the lifespan and climbing ability of Drosophila melanogaster

Perveen

Kumari

Raj

et al. 2021

JoBAZ

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“…The pupation travelling distance can be influenced by genetic traits and external factors such as moisture and environmental cues (Beltramí et al, 2010;Johnson and Carder, 2012;Narasimha et al, 2015;Sokolowski and Bauer, 1989). In addition, the distance to the pupation site has been used as a marker for fitness of the neuromuscular system to sense the surrounding environment and drive the crawling larva away from the food source to a safe location for the metamorphosis process to occur (Johnson and Carder, 2012;Joshi and Mueller, 1993), notably upon exposure of the developing larva to toxic compounds (Khatun et al, 2018;Sood et al, 2019). Accordingly, we measured the pupation distance by recording the position of pupas relative to the food level in four categories: I. within the food; II.…”

Section: Effect Of Dmso and Cps On Developmental Survivalmentioning

confidence: 99%

Motor dysfunction in Drosophila melanogaster as a biomarker for developmental neurotoxicity

Cabrita

Pereira

et al. 2021

Preprint

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Humans interact with numerous chemical compounds with direct health implications, with several able to induce developmental neurotoxicity (DNT), which bear developmental, behavioral, and cognitive consequences from a young age. Current guidelines for DNT testing are notably costly, time consuming, and unsuitable for testing large numbers of chemicals. Therefore, there is a need for adequate alternatives to conventional animal testing for neurotoxicity and DNT. Here we show that detailed kinematic analysis can provide a strong indicator for DNT, using known (chlorpyrifos, CPS) or putative (β-N-methylamino-L-alanine, BMAA) neurotoxic compounds. We exposed Drosophila melanogaster to these compounds during development and evaluated for common general toxicity — notably developmental survival and pupal positioning, together with the FlyWalker system, a detailed adult kinematics evaluation method.At concentrations that do not induce general toxicity, the solvent DMSO had a significant effect on kinematic parameters. Nonetheless, CPS not only induced developmental lethality but also significantly impaired coordination in comparison to DMSO, altering 16 motor parameters, validating the usefulness of our kinematic approach.Interestingly, BMAA, although not lethal during development, induced a dose-dependent motor decay, targeting most parameters in young adult animals, phenotypically resembling normally aged, non-exposed flies. This effect was subsequently attenuated during ageing, indicating an adaptive response. Furthermore, BMAA induced an abnormal terminal differentiation of leg motor neurons, without inducing degeneration, underpinning the observed altered mobility phenotype. Overall, our results support our kinematic approach as a novel, highly sensitive and reliable tool to assess potential DNT of chemical compounds.

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“…В работе Холл [Hall, 1969] по изучению возрастных изменений гексозоизомеразной, эстеразной, глюкозо-6-фосфат дегидрогеназной и фосфатазной активностей у D. melanogaster было показано, что эстеразная активность с возрастом увеличивалась, при этом у самцов обнаружено более значительное увеличение активности с возрастом (в 2,6 раза по сравнению с молодыми самцами), чем у самок (в 1,5 раза по сравнению с молодыми самками). В ряде работ представлены данные о том, что у самок D. melanogaster активность АХЭ ниже, чем у самцов [Khatuna et al, 2018;Hu et al, 2019].…”

Section: результаты и обсуждениеunclassified

Phosphatase and acetylcholinesterase activities in different stages of the life cycle of the housefly Musca domestica L.

et al. 2020

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Всероссийский научно-исследовательский институт ветеринарной энтомологии и арахнологии -филиал Федерального государственного бюджетного учреждения науки Федерального исследовательского центра Тюменского научного центра Сибирского отделения Российской академии наук, ул. Институтская 2, Тюмень 625041 Россия.

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Interplay of ROS and behavioral pattern in fluoride exposed Drosophila melanogaster

Cited by 36 publications

References 71 publications

Effects of sodium fluoride and Ocimum sanctum extract on the lifespan and climbing ability of Drosophila melanogaster

Effects of sodium fluoride and Ocimum sanctum extract on the lifespan and climbing ability of Drosophila melanogaster

Motor dysfunction in Drosophila melanogaster as a biomarker for developmental neurotoxicity

Phosphatase and acetylcholinesterase activities in different stages of the life cycle of the housefly Musca domestica L.

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