Interplay of negative and positive signals controls endoderm-specific expression of the ascidian Cititf1 gene promoter

Fanelli, Albertina; Lania, Gabriella; Lauro, Roberto Di

doi:10.1016/s0012-1606(03)00397-x

Cited by 10 publications

(9 citation statements)

References 19 publications

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“…EMSA assay has been performed as previously reported (Fanelli et al, 2003), using 5.0 l of in vitro synthesized Ci-Ets1/2 DBD protein, and labeled and competitor DNAs prepared by annealing the wildtype (Wt2) or mutant (Mut2) oligonucleotides (see Table S3 in the supplementary material).…”

Section: Electro Mobility Shift Assay (Emsa)mentioning

confidence: 99%

FGF/MAPK/Ets signaling renders pigment cell precursors competent to respond to Wnt signal by directly controllingCi-Tcftranscription

et al. 2011

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SUMMARYFGF and Wnt pathways constitute two fundamental signaling cascades, which appear to crosstalk in cooperative or antagonistic fashions in several developmental processes. In vertebrates, both cascades are involved in pigment cell development, but the possible interplay between FGF and Wnt remains to be elucidated. In this study, we have investigated the role of FGF and Wnt signaling in development of the pigment cells in the sensory organs of C. intestinalis. This species possesses the basic features of an ancestral chordate, thus sharing conserved molecular developmental mechanisms with vertebrates. Chemical and targeted perturbation approaches revealed that a FGF signal, spreading in time from early gastrulation to neural tube closure, is responsible for pigment cell precursor induction. This signal is transmitted via the MAPK pathway, which activates the Ci-Ets1/2 transcription factor. Targeted perturbation of Ci-TCF, a downstream factor of the canonical Wnt pathway, indicated its contribution to pigment cell differentiation Furthermore, analyses of the Ci-Tcf regulatory region revealed the involvement of the FGF effector, Ci-Ets1/2, in Ci-Tcf transcriptional regulation in pigment cell precursors. Our results indicate that both FGF and the canonical Wnt pathways are involved in C. intestinalis pigment cell induction and differentiation. Moreover, we present a case of direct transcriptional regulation exerted by the FGF signaling cascade, via the MAPK-ERK-Ets1/2, on the Wnt downstream gene CiTcf. Several examples of FGF/Wnt signaling crosstalk have been described in different developmental processes; however, to our knowledge, FGF-Wnt cross-interaction at the transcriptional level has never been previously reported. These findings further contribute to clarifying the multitude of FGF-Wnt pathway interactions.

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Section: Electro Mobility Shift Assay (Emsa)mentioning

confidence: 99%

FGF/MAPK/Ets signaling renders pigment cell precursors competent to respond to Wnt signal by directly controllingCi-Tcftranscription

et al. 2011

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“…Such approaches are now being applied to the early development of the heart (B. Davidson, W. Shi, and M. Levine, in prep. ), endoderm (Satou et al 2001;Fanelli et al 2003;Imai 2003;Oda-Ishii et al 2005), tail muscles (Yagi et al 2005), and stomodaeum (Christiaen et al 2005). In combination with the comprehensive gene-expression studies and functional assays discussed previously, these enhancer studies will set the stage for deciphering the basic gene networks in the Ciona embryo.…”

Section: Regulatory Interactions Underlying Ciona Neural Specificationmentioning

confidence: 99%

Unraveling genomic regulatory networks in the simple chordate, Ciona intestinalis

Shi¹,

Levine²,

Davidson³

2005

Genome Res.

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The draft genome of the primitive chordate, Ciona intestinalis, was published three years ago. Since then, significant progress has been made in utilizing Ciona's genomic and morphological simplicity to better understand conserved chordate developmental processes. Extensive annotation and sequencing of staged EST libraries make the Ciona genome one of the best annotated among those that are publicly available. The formation of the Ciona tadpole depends on simple, well-defined cellular lineages, and it is possible to trace the lineages of key chordate tissues such as the notochord and neural tube to the fertilized egg. Electroporation methods permit the targeted expression of regulatory genes and signaling molecules in defined cell lineages, as well as the rapid identification of regulatory DNAs underlying cell-specific gene expression. The recent sequencing of a second Ciona genome (C. savignyi) permits the use of simple alignment algorithms for the identification of conserved noncoding sequences, including microRNA genes and enhancers. Detailed expression profiles are now available for almost every gene that encodes a regulatory protein or cell-signaling molecule. The combination of gene-expression profiles, comparative genome analysis, and gene-disruption assays should permit the determination of high-resolution genomic regulatory networks underlying the specification of basic chordate tissues such as the heart, blood, notochord, and neural tube.

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Section: Endodermmentioning

confidence: 99%

“…cis-Regulatory regions responsible for the endoderm specific expression of Cititf1 have been isolated and characterized (Ristoratore et al, 1999;Fanelli et al, 2003). The 315-bp genomic fragment spanning from -355 to -41 relative to the transcription start site is sufficient to activate a heterologous promoter in a pattern essentially identical to that of the endogenous Cititf1 gene (Fanelli et al, 2003). This enhancer contains at least three distinct regulatory regions; two of which are responsible for activation of transcription in the endoderm and in the mesenchyme, respectively, while the third is a negative control region that represses transcription in mesenchyme.…”

Section: Endodermmentioning

confidence: 99%

“…Other basal promoters that have been used for functional assays of heterologous cis-regulatory sequences include those of Ci-Sna (Di , CiHox3 (Fanelli et al, 2003;Russo et al, 2004), HrMA4a (Bertrand et al, 2003), and Ci-Gsx (Bertrand et al, 2003). Another example of promoter activation by heterologous cis-regulatory sequences is enhancer trapping in the Ciona genomes using the Minos transposon.…”

Section: Heterologous Basal Promotersmentioning

confidence: 99%

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A Novel G Protein α Subunit in Embryo of the Ascidian, Halocynthia roretzi

Iwasa¹,

Mishima²,

Watari³

et al. 2003

Zoological Science

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-Ascidians, or sea squirts, are lower chordates, and share basic gene repertoires and many characteristics, both developmental and physiological, with vertebrates. Therefore, decoding cis -regulatory systems in ascidians will contribute toward elucidating the genetic regulatory systems underlying the developmental and physiological processes of vertebrates. cis -Regulatory DNAs can also be used for tissuespecific genetic manipulation, a powerful tool for studying ascidian development and physiology. Because the ascidian genome is compact compared with vertebrate genomes, both intergenic regions and introns are relatively small in ascidians. Short upstream intergenic regions contain a complete set of cis -regulatory elements for spatially regulated expression of a majority of ascidian genes. These features of the ascidian genome are a great advantage in identifying cis -regulatory sequences and in analyzing their functions. Function of cis -regulatory DNAs has been analyzed for a number of tissue-specific and developmentally regulated genes of ascidians by introducing promoter-reporter fusion constructs into ascidian embryos. The availability of the whole genome sequences of the two Ciona species, Ciona intestinalis and Ciona savignyi , facilitates comparative genomics approaches to identify cis -regulatory DNAs. Recent studies demonstrate that computational methods can help identify cis-regulatory elements in the ascidian genome. This review presents a comprehensive list of ascidian genes whose cis -regulatory regions have been subjected to functional analysis, and highlights the recent advances in bioinformatics and comparative genomics approaches to cis -regulatory systems in ascidians.

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Interplay of negative and positive signals controls endoderm-specific expression of the ascidian Cititf1 gene promoter

Cited by 10 publications

References 19 publications

FGF/MAPK/Ets signaling renders pigment cell precursors competent to respond to Wnt signal by directly controllingCi-Tcftranscription

FGF/MAPK/Ets signaling renders pigment cell precursors competent to respond to Wnt signal by directly controllingCi-Tcftranscription

Unraveling genomic regulatory networks in the simple chordate, Ciona intestinalis

A Novel G Protein α Subunit in Embryo of the Ascidian, Halocynthia roretzi

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