Interplay of intestinal microbiota and mucosal immunity in inflammatory bowel disease: a relationship of frenemies

Chen, Huimin; Li, Hongfen; Liu, Zhanju

doi:10.1177/1756284820935188

Cited by 19 publications

(14 citation statements)

References 83 publications

(116 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Discussionmentioning

confidence: 99%

“…Intestinal flora plays a regulatory role in host digestion, metabolism, immunity, and other physiological functions and is considered a new functional organ ( Ringel-Kulka, 2012 ; Wei et al., 2021 ). The changes in intestinal flora have been linked to inflammatory bowel disease, obesity, colorectal cancer, type 2 diabetes, and intestinal dysfunction ( Sabatino et al., 2017 ; Chen et al., 2020 ). The changes in the composition of calves’ intestinal flora destroy the immune barrier function mediated by intestinal microbes, increasing the susceptibility of the intestinal tract to pathogenic bacteria and endangering the animals’ health.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Early Pathogenic Escherichia coli Infection on the Intestinal Barrier and Immune Function in Newborn Calves

Wang

Simujide

Areum

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

We studied the effect of early pathogenic Escherichia coli infection on newborn calves’ intestinal barrier and immune function. A total of 64 newborn Holstein male calves (40–43 kg) were divided into two groups: normal (NG) and test (TG), each with 32 heads. At the beginning of the experiment, the TG calves were orally administered pathogenic E. coli O1 (2.5 × 1011 CFU/mL, 100 mL) to establish a calf diarrhea model. In contrast, the NG calves were given the same amount of normal saline. During the 30 d trial period, the feeding and management of the two groups remained constant. Enzyme-linked immunosorbent assay, quantification PCR, and high-throughput 16S rRNA sequencing technology were used to detect indicators related to the intestinal barrier and immune function in the calf serum and tissues. Pathogenic E. coli O1 had a significant effect on calf diarrhea in the TG; it increased the bovine diamine oxidase (P < 0.05) and endotoxin levels in the serum and decreased (P < 0.05) the intestinal trefoil factor (P < 0.05), Occludin, Claudin-1, and Zonula Occludens 1 (ZO-1) levels in the colon tissue, as well as downregulated the mRNA expression of Occludin, Claudin-1,and ZO-1 in the colon mucosa, leading to increased intestinal permeability and impaired intestinal barrier function. Additionally, pathogenic E. coli had a significant impact on the diversity of colonic microbial flora, increasing the relative abundance of Proteobacteria at the phylum level and decreasing the levels of Firmicutes and Bacteroides. At the genus level, the relative abundance of Escherichia and Shigella in the TG increased significantly (P < 0.05), whereas that of Bacteroides, Butyricicoccus, Rikenellaceae_RC9_gut_group, Blautia, and Lactobacillus was significantly decreased (P < 0.05). In addition, the level of IL-6 in the serum of the TG calves was significantly increased (P < 0.05), whereas the IL-4 and IL-10 levels were significantly decreased (P < 0.05), compared to those in the NG calves. Thus, pathogenic E. coli induced diarrhea early in life disrupts intestinal barrier and impairs immune function in calves.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of Early Pathogenic Escherichia coli Infection on the Intestinal Barrier and Immune Function in Newborn Calves

Wang

Simujide

Areum

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The human microbiome is constituted of a complex network of co-existing microbes, particularly within the human intestine [ 20 ]. Its composition varies throughout life, and intestinal homeostasis is preserved in healthy individuals [ 21 ]. However, failure to reach host–microbiota equilibrium can create a dysbiosis, inducing changes in gut microbial diversity and an inbalance between commensal and pathogenic microorganisms [ 22 ], which is linked to IBD susceptibility, onset and/or aggravation [ 21 , 23 ].…”

Section: Introduction To Inflammatory Bowel Diseases (Ibd)mentioning

confidence: 99%

“…Its composition varies throughout life, and intestinal homeostasis is preserved in healthy individuals [ 21 ]. However, failure to reach host–microbiota equilibrium can create a dysbiosis, inducing changes in gut microbial diversity and an inbalance between commensal and pathogenic microorganisms [ 22 ], which is linked to IBD susceptibility, onset and/or aggravation [ 21 , 23 ]. Prolonged gut dysbiosis enables pathogens to dominate the gut and favours the propagation of pro-IBD species, in parallel with the eradication of anti-IBD microbes [ 10 , 21 , 23 ].…”

Section: Introduction To Inflammatory Bowel Diseases (Ibd)mentioning

confidence: 99%

Drug Targeting of Inflammatory Bowel Diseases by Biomolecules

et al. 2021

View full text Add to dashboard Cite

Inflammatory bowel disease (IBD) is a group of disabling, destructive and incurable immune-mediated inflammatory diseases comprising Crohn’s disease (CD) and ulcerative colitis (UC), disorders that are highly prevalent worldwide and demand a large investment in healthcare. A persistent inflammatory state enables the dysfunction and destruction of healthy tissue, hindering the initiation and endurance of wound healing. Current treatments are ineffective at counteracting disease progression. Further, increased risk of serious side effects, other comorbidities and/or opportunistic infections highlight the need for effective treatment options. Gut microbiota, the key to preserving a healthy state, may, alternatively, increase a patient’s susceptibility to IBD onset and development given a relevant bacterial dysbiosis. Hence, the main goal of this review is to showcase the main conventional and emerging therapies for IBD, including microbiota-inspired untargeted and targeted approaches (such as phage therapy) to infection control. Special recognition is given to existing targeted strategies with biologics (via monoclonal antibodies, small molecules and nucleic acids) and stimuli-responsive (pH-, enzyme- and reactive oxygen species-triggered release), polymer-based nanomedicine that is specifically directed towards the regulation of inflammation overload (with some nanosystems additionally functionalized with carbohydrates or peptides directed towards M1-macrophages). The overall goal is to restore gut balance and decrease IBD’s societal impact.

show abstract

“…It also influences the development of extraintestinal organs, e.g., the cardiovascular system [ 11 ] or the nervous system [ 12 , 13 ]. Shifts in the composition of the microbiota may lead to dysbiosis in the gut, resulting in pathological processes [ 14 ] and can lead to gastrointestinal disorders, such as inflammatory bowel disease [ 15 , 16 ] and malignancies [ 17 , 18 ]. The microbiome may even affect disease etiology in other organ systems, such as obesity [ 19 , 20 ] and diabetes [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome

Wenderlein¹,

Böswald²,

Ulrich³

et al. 2021

Animals

View full text Add to dashboard Cite

The composition of the microbiome is subject to the host’s diet. In commercial laboratory mouse diets, different physical forms of the same diets are available, containing—according to their labels—identical ingredients and nutrient compositions. However, variations in nutrient composition and starch gelatinization due to production processes and their impact on digestibility have been described. In this study, a total of 48 C57BL/J6 mice were assigned to two equal groups and were fed diets (produced with different processes—extruded vs. pelleted) for eight weeks in two biological replicates. At the end of the experiment, samples were collected from five different gastrointestinal regions, including the stomach, small intestine, cecum, large intestine, and an extracorporeal region (feces), and the microbiome was analyzed with 16S rRNA gene amplicon sequencing. The replicates in both experiments differed significantly in their relative abundances of Muribaculaceae species. Furthermore, the gastrointestinal content of pellet-fed mice contained larger numbers of Lactobacillus species. These results indicate that starch gelatinization and ingredient composition significantly influence microbial makeup. In conclusion, different feed processing methods may affect fundamental digestive and metabolic processes, impacting animal experiments and biasing microbiome data.

show abstract

Interplay of intestinal microbiota and mucosal immunity in inflammatory bowel disease: a relationship of frenemies

Cited by 19 publications

References 83 publications

Effect of Early Pathogenic Escherichia coli Infection on the Intestinal Barrier and Immune Function in Newborn Calves

Effect of Early Pathogenic Escherichia coli Infection on the Intestinal Barrier and Immune Function in Newborn Calves

Drug Targeting of Inflammatory Bowel Diseases by Biomolecules

Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome

Contact Info

Product

Resources

About