The effect of bisacodyl on the treatment of rats with slow transit constipation
(STC) was studied. Forty-five female Wister rats were divided into control
group, STC group, and STC bisacodyl group. The immunohistochemical method was
used to determine interstitial cells of Cajal (ICC) and the expression of c-Kit
protein. Body mass and the number of defecations were significantly decreased in
the STC group compared with the control group on the 100th day after
diphenoxylate administration, while dry weight of feces was significantly
increased and the intestinal transit time was prolonged. There were significant
differences in the number of defecations, dry weight of feces, and intestinal
transit time among the three groups. The number of defecations was higher, dry
weight of feces was lower, and intestinal transit time was shorter in the STC
bisacodyl group compared to the STC group. In addition, ICC basement membrane
dissolution occurred in the colon wall of the STC group. The connection between
ICC and surrounding cells was destroyed, and the nucleus shrunken to different
degrees. Moreover, c-Kit expression in the STC group was significantly lower
than the control group. The connection between ICC and surrounding cells in the
STC bisacodyl group was significantly stronger than the STC group, and the
number of ICC and the expression of c-Kit were increased. Bisacodyl could reduce
the severity of STC in rats by increasing the number of ICC and the expression
of c-Kit.