Interplay between Ions, the Cytoskeleton, and Cell Wall Properties during Tip Growth

Bascom, Carlisle; Hepler, Peter K.; Bezanilla, Magdalena

doi:10.1104/pp.17.01466

Cited by 65 publications

(54 citation statements)

References 130 publications

(137 reference statements)

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“…Identifying direct regulators of channel activity will be of prime importance, in order to fully understand their function in growth regulation. Mechanical cues and other local signals, such as signaling lipids, ROS, RHO of plant GTPases (ROPs), pH changes and signals from the monitoring of cell wall integrity establish a regulatory network that may provide feed‐forward and feedback regulation to the Ca 2+ channels (Wolf et al ., ; Boisson‐Dernier et al ., ; Mendrinna and Persson, ; Mangano et al ., ; Bascom et al ., ; Kwon et al ., ). Knowing three CNGCs as central regulators of Ca 2+ oscillations in the root hair tip enables the study of the close relationship between the microenvironment, its membrane‐linked sensors and CNGC activities in more detail.…”

Section: Discussionmentioning

confidence: 99%

Multiple cyclic nucleotide‐gated channels coordinate calcium oscillations and polar growth of root hairs

et al. 2019

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Summary Calcium gradients underlie polarization in eukaryotic cells. In plants, a tip‐focused Ca2+‐gradient is fundamental for rapid and unidirectional cell expansion during epidermal root hair development. Here we report that three members of the cyclic nucleotide‐gated channel family are required to maintain cytosolic Ca2+ oscillations and the normal growth of root hairs. CNGC6, CNGC9 and CNGC14 were expressed in root hairs, with CNGC9 displaying the highest root hair specificity. In individual channel mutants, morphological defects including root hair swelling and branching, as well as bursting, were observed. The developmental phenotypes were amplified in the three cngc double mutant combinations. Finally, cngc6/9/14 triple mutants only developed bulging trichoblasts and could not form normal root hair protrusions because they burst after the transition to the rapid growth phase. Prior to developmental defects, single and double mutants showed increasingly disturbed patterns of Ca2+ oscillations. We conclude that CNGC6, CNGC9 and CNGC14 fulfill partially but not fully redundant functions in generating and maintaining tip‐focused Ca2+ oscillations, which are fundamental for proper root hair growth and polarity. Furthermore, the results suggest that these calmodulin‐binding and Ca2+‐permeable channels organize a robust tip‐focused oscillatory calcium gradient, which is not essential for root hair initiation but is required to control the integrity of the root hair after the transition to the rapid growth phase. Our findings also show that root hairs possess a large ability to compensate calcium‐signaling defects, and add new players to the regulatory network, which coordinates cell wall properties and cell expansion during polar root hair growth.

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Section: Discussionmentioning

confidence: 99%

Multiple cyclic nucleotide‐gated channels coordinate calcium oscillations and polar growth of root hairs

et al. 2019

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“…Because of this dual role, the action of PME could either increase or decrease CW stiffness and, in either case, is predicted to affect cell growth (Lionetti et al ., ; Senechal et al ., ). Methylation and de‐methylation are tightly regulated and rely not only on the localized expression of specific PME isoforms and the local presence of calcium ions (Bascom et al ., ) but also on the localized expression of PME inhibitors (PMEI) (Di Matteo et al ., ). Overexpression of PMEI causes changes in growth behavior, through the activation of the BR signaling pathway that in turn enhances transcription of CW‐modifying genes, including PME genes (Wolf, ).…”

Section: Developmentmentioning

confidence: 99%

Cell wall traits that influence plant development, immunity, and bioconversion

et al. 2019

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Summary The architecture of the plant cell wall is highly dynamic, being substantially re‐modeled during growth and development. Cell walls determine the size and shape of cells and contribute to the functional specialization of tissues and organs. Beyond the physiological dynamics, the wall structure undergoes changes upon biotic or abiotic stresses. In this review several cell wall traits, mainly related to pectin, one of the major matrix components, will be discussed in relation to plant development, immunity and industrial bioconversion of biomass, especially for energy production. Plant cell walls are a source of oligosaccharide fragments with a signaling function for both development and immunity. Sensing cell wall damage, sometimes through the perception of released damage‐associated molecular patterns (DAMPs), is crucial for some developmental and immunity responses. Methodological advances that are expected to deepen our knowledge of cell wall (CW) biology will also be presented.

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“…However, in nature, precise spatiotemporal control of supramolecular self‐assembly is important for the modulation of the conditions and the chemical environment of critical biochemical processes . For example, spatially localized supramolecular polymerization is crucial for the transduction of anisotropic mechanical forces, such as in protein contractile rings, which must be assembled in very specific locations to ensure the correct cell membrane contractions . A range of simplified confinement synthetic models have been recently designed to understand these polymerization processes and their distribution between the core or the cortex of these artificial containers –.…”

Section: Introductionmentioning

confidence: 99%

Spatially Controlled Supramolecular Polymerization of Peptide Nanotubes by Microfluidics

Méndez‐Ardoy

Bayón‐Fernández

et al. 2020

Angewandte Chemie

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Despite the importance of spatially resolved selfassembly for molecular machines,t he spatial control of supramolecular polymerization with synthetic monomers had not been experimentally established. Now,amicrofluidicregulated tandem process of supramolecular polymerization and droplet encapsulation is used to control the position of selfassembled microfibrillar bundles of cyclic peptide nanotubes in water droplets.T his method allows the precise preferential localization of fibers either at the interface or into the core of the droplets.U Vabsorbance,c ircular dichroism and fluorescence microscopyindicated that the microfluidic control of the stimuli (changes in pH or ionic strength) can be employed to adjust the packingd egree and the spatial position of microfibrillar bundles of cyclic peptide nanotubes.Additionally,this spatially organized supramolecular polymerization of peptide nanotubes was applied in the assembly of highly ordered twodimensional droplet networks.Supportinginformation and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.

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Interplay between Ions, the Cytoskeleton, and Cell Wall Properties during Tip Growth

Cited by 65 publications

References 130 publications

Multiple cyclic nucleotide‐gated channels coordinate calcium oscillations and polar growth of root hairs

Multiple cyclic nucleotide‐gated channels coordinate calcium oscillations and polar growth of root hairs

Cell wall traits that influence plant development, immunity, and bioconversion

Spatially Controlled Supramolecular Polymerization of Peptide Nanotubes by Microfluidics

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