Interplay between estrogen-related receptors and steroidogenesis-controlling molecules in adrenals. In vivo and in vitro study

Pacwa, Anna; Górowska-Wójtowicz, Ewelina; Ptak, Anna; Pawlicki, Piotr; Miłoń, Agnieszka; Sekuła, Małgorzata; Lesniak, Klaudia; Bilińska, Barbara; Hejmej, Anna; Kotula–Balak, Małgorzata

doi:10.1016/j.acthis.2018.05.007

Cited by 6 publications

(6 citation statements)

References 67 publications

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“…In this study, both B‐Raf and C‐Raf were observed in GCs. In addition, previous studies had been shown that Raf mediated steroidogenesis in mouse Leydig cells (Gorowska‐Wojtowicz et al, ) or human adrenocortical cells (Pacwa et al, ; Wu et al, ). This evidence supports the links between Raf and the biosynthesis of E 2 , T or P 4 .…”

Section: Discussionmentioning

confidence: 95%

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Raf‐ERK1/2 signalling pathways mediate steroid hormone synthesis in bovine ovarian granulosa cells

Jiang

et al. 2019

Reprod Domestic Animals

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Contents Steroid hormones are required for normal reproductive function of female. The aim of this study was to investigate the role of Raf‐ERK1/2 on steroid hormone synthesis in bovine ovarian granulosa cells. Immunohistochemistry assay showed that both B‐Raf and C‐Raf were expressed in granulosa cells, theca cells and Sertoli cells. The protein expression of Raf or ERK1/2 was clearly decreased by Raf inhibitor GSK2118436 or ERK1/2 inhibitor SCH772984, respectively (p < 0.05). In addition, western blotting was performed for investigating the crosstalk between Raf and ERK1/2, the data showed that Raf positively regulated ERK1/2, whereas ERK1/2 had a negative feedback effect on Raf. The biosynthesis of oestradiol or testosterone was significantly decreased by treatment with GSK2118436 or SCH772984 (p < 0.05). Conversely, the progesterone biosynthesis was clearly increased by treatment with those inhibitors (p < 0.05). Furthermore, the mRNA expression of STAR, aromatase and CYP17 was blocked by Raf‐ERK1/2 signalling inhibition, which oppositely induced the mRNA expression of CYP11. Together, these findings suggested that Raf‐ERK1/2 signalling pathways mediate steroid hormone synthesis via affecting the expression of steroidogenic enzymes.

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Section: Discussionmentioning

confidence: 95%

“…In this study, both B-Raf and C-Raf were observed in GCs. In addition, previous studies had been shown that Raf mediated steroidogenesis in mouse Leydig cells or human adrenocortical cells (Pacwa et al, 2018;Wu et al, 2002).…”

Section: Discussionmentioning

confidence: 96%

Raf‐ERK1/2 signalling pathways mediate steroid hormone synthesis in bovine ovarian granulosa cells

Jiang

et al. 2019

Reprod Domestic Animals

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“…An extensive tissue distribution pattern of 17βHSD8 was also found in humans ( 55 ), mice ( 56 ), and scallops ( 57 ). Similar to StAR3 , ERR was expressed in all of the examined tissues, and the relatively low levels in the hepatopancreas and plumose setae may corroborate ERR involvement in the transcriptional regulation of StAR ( 58 ).…”

Section: Discussionmentioning

confidence: 73%

17β-Estradiol (E2) may be involved in the mode of crustacean female sex hormone (CFSH) action in the blue crab, Callinectes sapidus

Wang

Blaney

et al. 2022

Front. Endocrinol.

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17β-estradiol (E2) has been proved to control reproduction, sexual differentiation, and the development of the secondary sexual characteristics of vertebrate females. In decapod crustacean species, crustacean female sex hormone (CFSH), a protein hormone, is required for developing adult-specific ovigerous setae for embryo brooding and gonophores for mating at the blue crab Callinectes sapidus puberty molting. However, it is unclear that whether the mode of CFSH action involves a vertebrate-type sex steroid hormone in crustaceans. To this end, E2 levels were first measured using a competitive ELISA in the hemolymph and the potential CFSH target tissues from both prepuberty and adult females; the presence of E2 was further confirmed with a liquid chromatography tandem mass spectrometry method. Then, the cDNAs of the following genes known to be associated with vertebrate steroidogenic pathways were isolated: StAR-related lipid transfer protein 3 (StAR3); 3β-hydroxysteroid dehydrogenase (3βHSD); two isoforms of 17β-hydroxysteroid dehydrogenase 8 (17βHSD8); and, estradiol-related receptor (ERR). RT-PCR analysis revealed that these genes were widely distributed in the eyestalk ganglia, hepatopancreas, brain, ovary, spermathecae, ovigerous and plumose setae tissues of adult females. The 17βHSD8 transcripts were localized in the follicle cells, the periphery of the nuclear membrane of primary oocytes, and yolk granules of the vitellogenic oocytes using in situ hybridization, and the corresponding protein was detected in the follicle cells and ooplasm of primary oocytes using immunohistochemistry. Furthermore, the adult females injected with CFSH-dsRNA (n = 30 times) had E2 and StAR3 transcripts levels lower in the ovigerous and plumose setae, spermathecae than controls. These results suggested that the mode of CFSH action in C. sapidus might involve E2 in these adult-female-specific tissues.

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“…In feminizing adrenal tumors, the mechanism of estrogen production has been proposed to be due to either peripheral aromatization of androgens or estrogen production from the tumor itself (Phornphutkul et al, 2001). There are poor number of studies, including ours, reported regulatory roles and/or mutual interactions of adrenal estrogen signaling through both canonical and noncanonical estrogen receptors for males (Lagunas et al, 2022;Pacwa et al, 2018;Pawlicki et al, 2023;Trejter et al, 2015). Pharmacological blockage of androgen and GPER estrogen signaling leads to permanent modifications of the neuroendocrine functions of the pituitary and adrenal glands in the adulthood of both sexes (Lagunas et al, 2022).…”

Section: Discussionmentioning

confidence: 89%

“…Till now, only a small portion of studies including ours, focused on the sex hormone regulation of the adrenal cortex. The adrenal cortex expresses all estrogen receptor subtypes, both canonical and noncanonical (Caroccia et al, 2016; Pacwa et al, 2018; Pawlicki et al, 2023; Trejter et al, 2015). In this gland, estrogens are especially involved in the regulation of cell proliferation or aldosterone synthesis (Caroccia et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Is membrane androgen and estrogen receptor signaling imperative in the governing function of the adrenal cortex in the Eurasian beaver (Castor fiber L.)?

Lonc,

Hrabia,

Krakowska

et al. 2024

J Exp Zool Pt A

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There is a need to fully know the physiology of Eurasian beaver due to its essential role in environmental homeostasis. However, a “human factor“ impacts this, including stress conditions and environmental pollution. Adrenal glands protect these all. The regulation of endocrine processes by nonclassical androgen and estrogen signaling, the first and fastest control, is still a matter of research. The specific analyses performed here in mature female and male beaver adrenals contained: anatomical and histological examinations, expression and localization of membrane androgen receptor (zinc transporter, Zinc‐ and Iron‐like protein 9; ZIP9) and membrane estrogen receptor coupled with G protein (GPER), and measurement of zinc (Zn2+) and copper (Ca2+) ion levels and corticosterone levels. We revealed normal anatomical localization, size, and tissue histology in female and male beavers, respectively. Equally, ZIP9 and GPER were localized in the membrane of all adrenal cortex cells. The protein expression of these receptors was higher (p < 0.001) in male than female adrenal cortex cells. Similarly, Zn2+ and Ca2+ ion levels were higher (p < 0.05, p < 0.01) in male than female adrenal cortex. The increased corticosterone levels (p < 0.001) were detected in the adrenal cortex of females when compared to males. The present study is the first to report the presence of nonclassical androgen and estrogen signaling and its possible regulatory function in the adrenal cortex of Eurasian beavers. We assume that this first‐activated and fast‐transmitted regulation can be important in the context of the effect of environmental physical and chemical stressors especially on adrenal cortex cells. The beaver adrenals may constitute an additional supplementary model for searching for universal mechanisms of adrenal cortex physiology and diseases.

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Interplay between estrogen-related receptors and steroidogenesis-controlling molecules in adrenals. In vivo and in vitro study

Cited by 6 publications

References 67 publications

Raf‐ERK1/2 signalling pathways mediate steroid hormone synthesis in bovine ovarian granulosa cells

Raf‐ERK1/2 signalling pathways mediate steroid hormone synthesis in bovine ovarian granulosa cells

17β-Estradiol (E2) may be involved in the mode of crustacean female sex hormone (CFSH) action in the blue crab, Callinectes sapidus

Is membrane androgen and estrogen receptor signaling imperative in the governing function of the adrenal cortex in the Eurasian beaver (Castor fiber L.)?

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