Interplay between collagenase and undescended testes in Adamts16 knockout rats

Sarila, Gulcan; Bao, Ting; Abeydeera, Sanduni Amaya; Li, Ruili; Mell, Blair; Joe, Bina; Catubig, Angelique; Hutson, John M.

doi:10.1016/j.jpedsurg.2019.12.019

Cited by 7 publications

(4 citation statements)

References 19 publications

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“…ADAMTS16 and COMP are extracellular matrix proteins. ADAMTS16 can migrate to the testis, destroying collagen fibrous tissue in the fat pad of the inguinal scrotum ( 36 ). Furthermore, a mutation in COMP have been found to affect the balance between adipogenesis and bone formation ( 37 ).…”

Section: Discussionmentioning

confidence: 99%

Screening of Bovine Tissue-Specific Expressed Genes and Identification of Genetic Variation Within an Adipose Tissue-Specific lncRNA Gene

Zhang

Jiang

et al. 2022

Front. Vet. Sci.

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Global classification of bovine genes is important for studies of biology and tissue-specific gene editing. Herein, we classified the tissue-specific expressed genes and uncovered an important variation in the promoter region of an adipose tissue-specific lncRNA gene. Statistical analysis demonstrated that the number of genes specifically expressed in the brain was the highest, while it was lowest in the adipose tissues. A total of 1,575 genes were found to be significantly higher expressed in adipose tissues. Bioinformatic analysis and qRT-PCR were used to uncover the expression profiles of the 23 adipose tissue-specific and highly expressed genes in 8 tissues. The results showed that most of the 23 genes have higher expression level in adipose tissue. Besides, we detected a 12 bp insertion/deletion (indel) variation (rs720343880) in the promoter region of an adipose tissue-specific lncRNA gene (LOC100847835). The different genotypes of this variation were associated with carcass traits of cattle. Therefore, the outcomes of the present study can be used as a starting point to explore the development of cattle organs and tissues, as well as to improve the quality of cattle products.

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Section: Discussionmentioning

confidence: 99%

Screening of Bovine Tissue-Specific Expressed Genes and Identification of Genetic Variation Within an Adipose Tissue-Specific lncRNA Gene

Zhang

Jiang

et al. 2022

Front. Vet. Sci.

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“…Briefly, IHC staining was analyzed using the “IHC Toolbox” plugin in Image J (Shu et al 2016 ), and the co-localization ratio was calculated by the confocal microscope system (Leica TCS SP8 X, Germany). Semi-quantitative analysis of Masson's trichrome staining was performed using “color deconvolution” plugin in Image J (Ruifrok and Johnston 2001 ), as described by previous studies (Sarila et al 2020 ; Pazos et al 2010 ). The blue-stained collagen fibers were measured as the collagen volume fraction (CVF) of the region of interest.…”

Section: Methodsmentioning

confidence: 99%

OLMALINC/OCT4/BMP2 axis enhances osteogenic-like phenotype of renal interstitial fibroblasts to participate in Randall’s plaque formation

Zhu

Huang

Jiang

et al. 2022

Mol Med

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Background Randall’s plaques (RP) are identified as anchored sites for kidney calcium oxalate stones, but the mechanism remains unclear. Given the importance of osteogenic-like cells in RP formation and OCT4 in reprogramming differentiated cells to osteoblasts, the current study explored the potential role of OCT4 in RP formation. Methods OCT4 and biomineralization were evaluated in RP, and immunofluorescence co-staining was performed to identify these cells with alteration of OCT4 and osteogenic markers. Based on the analysis of tissue, we further investigated the mechanism of OCT4 in regulating osteogenic-like differentiation of primary human renal interstitial fibroblasts (hRIFs) in vitro and vivo. Results We identified the upregulated OCT4 in RP, with a positive correlation to osteogenic markers. Interestingly, fibroblast marker Vimentin was partially co-localized with upregulated OCT4 and osteogenic markers in RP. Further investigations revealed that OCT4 significantly enhanced the osteogenic-like phenotype of hRIFs in vitro and in vivo. Mechanically, OCT4 directly bound to BMP2 promoter and facilitated its CpG island demethylation to transcriptionally promote BMP2 expression. Furthermore, combination of RIP and RNA profiling uncovered that lncRNA OLMALINC physically interacted with OCT4 to promote its stabilization via disrupting the ubiquitination. Additionally, OLMALINC was upregulated in fibroblasts in RP visualized by FISH, and a positive correlation was revealed between OLMALINC and OCT4 in RP. Conclusions The upregulation of OCT4 in hRIFs was a pathological feature of RP formation, and OLMALINC/OCT4/BMP2 axis facilitated hRIFs to acquire osteogenic-like phenotype under osteogenic conditions, through which the pathway might participate in RP formation. Our findings opened up a new avenue to better understand RP formation in which osteogenic-like process was partially triggered by lncRNAs and pluripotency maintenance related genes.

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“…Jacobi et al [ 61 ] reported there was expression of ADAMTS16 in the testes of mouse embryos. Likewise, alterations in ADAMTS16 led to cryptorchidism and infertility in male rats [ 60 , 62 ]. Nevertheless, as reported by Livermore et al [ 63 ], the silencing of ADAMTS16 via CRISPR/Cas9 gene editing led to the production of mice that were fertile, although with smaller testicles.…”

Section: Adamts and Fertility In Malesmentioning

confidence: 99%

ADAMTS Proteases: Importance in Animal Reproduction

Hernández-Delgado

Félix-Portillo

Martínez-Quintana

2023

Genes

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Many reproductive physiological processes, such as folliculogenesis, ovulation, implantation, and fertilization, require the synthesis, remodeling, and degradation of the extracellular matrix (ECM). The ADAMTS (A Disintegrin and Metalloproteinase with Thrombospondin Motifs) family genes code for key metalloproteinases in the remodeling process of different ECM. Several genes of this family encode for proteins with important functions in reproductive processes; in particular, ADAMTS1, 4, 5 and 9 are genes that are differentially expressed in cell types and the physiological stages of reproductive tissues. ADAMTS enzymes degrade proteoglycans in the ECM of the follicles so that the oocytes can be released and regulate follicle development during folliculogenesis, favoring the action of essential growth factors, such as FGF-2, FGF-7 and GDF-9. The transcriptional regulation of ADAMTS1 and 9 in preovulatory follicles occurs because of the gonadotropin surge in preovulatory follicles, via the progesterone/progesterone receptor complex. In addition, in the case of ADAMTS1, pathways involving protein kinase A (PKA), extracellular signal regulated protein kinase (ERK1/2) and the epidermal growth factor receptor (EGFR) might contribute to ECM regulation. Different Omic studies indicate the importance of genes of the ADAMTS family from a reproductive aspect. ADAMTS genes could serve as biomarkers for genetic improvement and contribute to enhance fertility and animal reproduction; however, more research related to these genes, the synthesis of proteins encoded by these genes, and regulation in farm animals is needed.

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Interplay between collagenase and undescended testes in Adamts16 knockout rats

Cited by 7 publications

References 19 publications

Screening of Bovine Tissue-Specific Expressed Genes and Identification of Genetic Variation Within an Adipose Tissue-Specific lncRNA Gene

Screening of Bovine Tissue-Specific Expressed Genes and Identification of Genetic Variation Within an Adipose Tissue-Specific lncRNA Gene

OLMALINC/OCT4/BMP2 axis enhances osteogenic-like phenotype of renal interstitial fibroblasts to participate in Randall’s plaque formation

ADAMTS Proteases: Importance in Animal Reproduction

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