Interplay between autophagy and apoptosis mediated by copper oxide nanoparticles in human breast cancer cells MCF7

Laha, Dipranjan; Pramanik, Arindam; Maity, Jyotirindra; Mukherjee, Amitava; Pramanik, Panchanan; Laskar, Aparna; Karmakar, Parimal

doi:10.1016/j.bbagen.2013.08.011

Cited by 120 publications

(65 citation statements)

References 41 publications

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“…These results indicate that the cytoxicity of CuO nanoparticles may involve the autophagic pathway in A549 cells. These results support the results reported by Laha et al [80], in which CuO nanoparticles were incubated with cancer cells.…”

Section: Copper Oxide (Cuo Cu2o) Nanoparticlessupporting

confidence: 93%

“…Laha et al [80] synthesized CuO nanoparticles (30 nm) by biophysical methods, and reported that CuO nanoparticles induced autophagy in a human breast cancer cell line (MCF7) in a time-and dose-dependent manner. Siddiqui et al [81] reported that CuO nanoparticles (average size 22 nm) induced cytotoxicity in human hepatocellular carcinoma (HepG2) cells in a dose-dependent manner (2-50 mg/mL) and reported that tumor suppressor gene p53 and apoptotic gene caspase-3 were upregulated upon exposure to CuO nanoparticles.…”

Section: Copper Oxide (Cuo Cu2o) Nanoparticlesmentioning

confidence: 99%

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Nanotoxicology of Metal Oxide Nanoparticles

Seabra

Durán

2015

Metals

177

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This review discusses recent advances in the synthesis, characterization and toxicity of metal oxide nanoparticles obtained mainly through biogenic (green) processes. The in vitro and in vivo toxicities of these oxides are discussed including a consideration of the factors important for safe use of these nanomaterials. The toxicities of different metal oxide nanoparticles are compared. The importance of biogenic synthesized metal oxide nanoparticles has been increasing in recent years; however, more studies aimed at better characterizing the potent toxicity of these nanoparticles are still necessary for nanosafely considerations and environmental perspectives. In this context, this review aims to inspire new research in the design of green approaches to obtain metal oxide nanoparticles for biomedical and technological applications and to highlight the critical need to fully investigate the nanotoxicity of these particles. OPEN ACCESSMetals 2015, 5 935

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Section: Copper Oxide (Cuo Cu2o) Nanoparticlessupporting

confidence: 93%

Section: Copper Oxide (Cuo Cu2o) Nanoparticlesmentioning

confidence: 99%

Nanotoxicology of Metal Oxide Nanoparticles

Seabra

Durán

2015

Metals

177

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“…6C). It is well known that the active caspase cascade cleaves the DNA repair enzyme poly-ADP-ribose polymerase (PARP), resulting in its inactivation in apoptotic cells (Laha et al 2014). The shRNA-mediated knockdown of SRPK1 expression increased the cleavage of PARP in the MCF-7 cells treated with sodium arsenite, relative to that observed in the cells transfected with the control plasmid (Fig.…”

Section: Utilization Of Mcl-1 Exon 2 Is Regulated By Rbm4 Through Binmentioning

confidence: 91%

Elevated SRPK1 lessens apoptosis in breast cancer cells through RBM4-regulated splicing events

Lin

Tarn

et al. 2014

RNA

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Imbalanced splicing of premessenger RNA is typical of tumorous malignancies, and the regulatory mechanisms involved in several tumorigenesis-associated splicing events are identified. Elevated expression of serine-arginine protein kinase 1 (SRPK1) may participate in the pathway responsible for the dysregulation of splicing events in malignant tumor cells. In this study, we observed a correlation between the cytoplasmic accumulation of RNA-binding motif protein 4 (RBM4) and up-regulated SRPK1 in breast cancer cells. The production of the IR-B and MCL-1 S transcripts was induced separately by the overexpression of RBM4 and SRPK1 gene silencing. Overexpressed RBM4 simultaneously bound to the CU-rich elements within the MCL-1 exon2 and the downstream intron, which subsequently facilitated the exclusion of the regulated exon. Breast cancer cells are deprived of apoptotic resistance through the RBM4-mediated up-regulation of the IR-B and MCL-1 S transcripts. These findings suggest that the splicing events regulated by the SRPK1-RMB4 network may contribute to tumorigenesis through altered sensitivity to apoptotic signals in breast cancer cells.

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“…In human breast cancer (MCF-7) cells, CONPs were found to induce autophagy. This may be a cellular defense against CONP toxicity because the inhibition of autophagy returns the cellular response to apoptosis, as revealed by the cleavage of PARP, dephosphorylation of Bad, and increase of the cleavage product of caspase 3 (Laha et al 2014). Moreover, CONPs inhibit angiogenesis via the downregulation of VEGFR2 expression in primary human umbilical vein endothelial cells (HUVECs).…”

Section: Apoptotic Mode Of Cancer Cell Death By Metal-based Npsmentioning

confidence: 98%

Selective cancer-killing ability of metal-based nanoparticles: implications for cancer therapy

et al. 2015

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There has been little focus on the promising ability of metal-based nanoparticles (NPs) to kill cancer cells while sparing normal cells. Many in vitro and in vivo reports suggest that certain metal-based NPs are able to induce apoptosis and autophagy in cancer cells at specific concentrations that are not significantly toxic to non-cancerous cells. Those NPs are thought to exploit the oxidative stress conditions that prevail in cancer cells, which are largely exhausted of antioxidant ability. This review considers the induction of reactive oxygen species (ROS) by metal-based NPs as a mechanism for the specific killing of cancer cells. The article concomitantly provides a comprehensive description of the important pathways and molecules leading to programmed cell death (PCD), which occurs mainly via apoptosis, autophagy, and necroptosis. The PCD pathways are followed as ROS-burdened cancer cells succumb to ROS-generating metal-based NPs. Exploration of nanotechnology interventions in anticancer therapy demands further research into the mechanism of intracellular induction of ROS by metal-based NPs. Furthermore, the induction of ROS by NPs should be strictly controlled if ROS-based therapy is to become a paradigm in cancer therapy.

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Interplay between autophagy and apoptosis mediated by copper oxide nanoparticles in human breast cancer cells MCF7

Cited by 120 publications

References 41 publications

Nanotoxicology of Metal Oxide Nanoparticles

Nanotoxicology of Metal Oxide Nanoparticles

Elevated SRPK1 lessens apoptosis in breast cancer cells through RBM4-regulated splicing events

Selective cancer-killing ability of metal-based nanoparticles: implications for cancer therapy

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