INTERPHASE FLUORESCENCEIN SITU HYBRIDIZATION DETECTION OF t(2;13)(q35;q14) IN ALVEOLAR RHABDOMYOSARCOMA—A DIAGNOSTIC TOOL IN MINIMALLY INVASIVE BIOPSIES

Supplementary Materials and Methods from Antitumor Activity of Sustained N-Myc Reduction in Rhabdomyosarcomas and Transcriptional Block by Antigene Therapy

“…MYCN gene copy number and N-Myc immunohistochemistry analyses. The fusion gene status was determined by FISH, as previously described (McManus et al, 1996).…”

“…Fingerprinting carried out within six months of the experiments described were matched with those available from repositories which hold the lines (RH30, RD and RH4) or fingerprints produced after arrival of the cell lines in our laboratories.MYCN gene copy number and N-Myc immunohistochemistry analyses. The fusion gene status was determined by FISH, as previously described (McManus et al, 1996).Cell lines known to express N-Myc protein were used as positive controls for the immunohistochemistry on the TMAs as well as primary material and cell lines that did not (normal skeletal muscle, normal liver, RMS cell line (MYCN amplified), RD cell line (no N-Myc expression detected on western blots). Slides were only scored where convincing nuclear staining was obtained in the positive control cores, and where negative controls remained negative.…”

Supplementary Materials and Methods from Antitumor Activity of Sustained N-Myc Reduction in Rhabdomyosarcomas and Transcriptional Block by Antigene Therapy

“…MYCN gene copy number and N-Myc immunohistochemistry analyses. The fusion gene status was determined by FISH, as previously described (McManus et al, 1996).…”

“…Fingerprinting carried out within six months of the experiments described were matched with those available from repositories which hold the lines (RH30, RD and RH4) or fingerprints produced after arrival of the cell lines in our laboratories.MYCN gene copy number and N-Myc immunohistochemistry analyses. The fusion gene status was determined by FISH, as previously described (McManus et al, 1996).Cell lines known to express N-Myc protein were used as positive controls for the immunohistochemistry on the TMAs as well as primary material and cell lines that did not (normal skeletal muscle, normal liver, RMS cell line (MYCN amplified), RD cell line (no N-Myc expression detected on western blots). Slides were only scored where convincing nuclear staining was obtained in the positive control cores, and where negative controls remained negative.…”