Interparticle Interactions in Southern Bean Mosaic Virus Suspensions

Abstract

Small angle x-ray scattering data have been obtained for concentrated suspensions of southern bean mosaic virus containing approximately 20, 10, and 5 gm virus per 100 ml of suspension. In addition, the scattering from independent virus particles was determined from the scattering curves for dilute suspensions. The Zernike-Prins equation was used to compute the pair correlation functions for the concentrated suspensions. The pair correlation functions indicated relatively weak interparticle forces. The effective distance of closest approach of two particles was appreciably greater than the particle diameter, which was approximatley 285 Å. The interaction potential energy for a pair of molecules was estimated by three different approximate theories.

Small-angle X-ray scattering from metal-ammonia solutions

“…I(k) can then be written in terms of the Zernike-Prins equation for a one-component system plus a constant term, B, to account for the scattering by the medium. If the scattering factor, f21(k), for type 1 particles can be determined, the pair correlation function, g(r), can be determined by numerical evaluation of the relation (Schmidt & Taylor, 1967) g…”

Determination of the structure factor for concentrated silica dispersions using small angle x-ray scattering. II. Experiment

“…It has been used extensively to study such a variety of systems as solid polymers, catalysts, and suspensions of hiological macromolecules. Schmidt and Taylor [2], studied the interparticle interactions of southern bean mosaic virus suspensions and Brady and Gravatt [3] did measurements on suspensions of polystyrene latices which were dried to give solid films, assuming that the liquid structure would be maintained during the drying procedure. For suspensions of biological macromolecules, which, if carefully purified, consist of one single molecular species this leads to very detailed structural information.…”

Etude du gonflement des vermiculites–ornithine en solution saline par analyse de la diffusion des rayons X aux petits angles. Méthode d'interprétation et recherche des paramètres d'ordre

“…l'origine de l'espace r6ciproque, ce qui est g6n6rale-ment obtenu par extrapolation de la courbe G,xp. La distance moyenne dr est alors entach6e d'une erreur difficilement quantifiable qui se r6percute sur la courbe W(R) (Schmidt & Taylor, 1967) et rend la d+convolution de la courbe d'autant plus hasardeuse.…”