Interobserver variation among histopathologic evaluations of intestinal tissues from dogs and cats

Willard, Michael D.; Jergens, Albert E.; Duncan, Robert B.; Leib, Michael S.; McCracken, Malcolm D.; DeNovo, Robert C.; Helman, R. G.; Slater, Margaret R.; Harbison, Jacque L

doi:10.2460/javma.2002.220.1177

Cited by 143 publications

(151 citation statements)

References 21 publications

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“…Intestinal tissues have been reported to have substantial variation in their histopathologic presentations, as evaluated by pathologists [11]. To our knowledge, there is currently no histopathological criterion for the diagnosis of canine alimentary lymphoma.…”

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confidence: 99%

Sensitivity for the Detection of a Clonally Rearranged Antigen Receptor Gene in Endoscopically Obtained Biopsy Specimens from Canine Alimentary Lymphoma

Fukushima

Ohno

Koshino-Goto

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. Canine alimentary lymphoma is currently diagnosed on the basis of findings of cytological or histopathological examination. However, it is often difficult to histopathologically distinguish alimentary lymphoma from lymphocytic-plasmacytic enteritis. Recently, the application of polymerase chain reaction for antigen receptor gene rearrangement (PARR) has been reported. In the present study, we assess the sensitivity of PARR analysis in diagnosing canine alimentary lymphoma using endoscopically obtained biopsy specimens from 12 dogs that were histopathologically diagnosed as having lymphoma. The sensitivity of PARR analysis in diagnosing alimentary lymphoma was found to be 66.7%, which was lower than that of other lymphoid malignancies. A combination of histipathological examination and findings of PARR analysis may improve the diagnostic accuracy.KEY WORDS: cancer diagnosis, canine, gastro-intestinal disease, lymphoma/leukemia, PCR.

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confidence: 99%

Sensitivity for the Detection of a Clonally Rearranged Antigen Receptor Gene in Endoscopically Obtained Biopsy Specimens from Canine Alimentary Lymphoma

Fukushima

Ohno

Koshino-Goto

et al. 2009

The Journal of Veterinary Medical Science

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“…Histopathological analyses of intestinal samples report increased infiltration of the lamina propria of the intestinal mucosa with eosinophils, lymphocytes, plasma cells and (or) neutrophils (German et al, 2001;Craven et al, 2004). However, histopathological reports are variable and do not necessarily correlate with the clinical presentation of the animals (Willard et al, 2002). Based on the combined results of clinical examination, histopathology, responsiveness to treatment and contingent relapses, two main differential diagnoses of CE -food responsive diarrhea (FRD) or inflammatory bowel disease (IBD) -can potentially be distinguished (Willard et al, 2002).…”

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confidence: 99%

“…However, histopathological reports are variable and do not necessarily correlate with the clinical presentation of the animals (Willard et al, 2002). Based on the combined results of clinical examination, histopathology, responsiveness to treatment and contingent relapses, two main differential diagnoses of CE -food responsive diarrhea (FRD) or inflammatory bowel disease (IBD) -can potentially be distinguished (Willard et al, 2002). However, difficulties in diagnosing FRD or IBD remain and the usefulness for follow-up and prognosis in dogs suffering from CE is questionable.…”

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confidence: 99%

Cytokine mRNA abundance in intestinal biopsies from dogs with chronic diarrhea

Sauter¹,

Allenspach²,

Blum³

2007

Vet. Med. - Czech

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ABSTRACT:The differentiation between inflammatory bowel disease (IBD) and food responsive diarrhea (FRD) is difficult and no objective markers are available. We postulated that patterns of selected key cytokines would help to objectively differentiate between the two subcategories of chronic enteropathies in dogs. We studied mRNA patterns of selected cytokines in dogs with chronic enteropathies. Ten dogs with FRD (= group FRDbef ) and seven dogs with IBD (= group IBDbef ) were presented for endoscopy at the Small Animal Clinic, University of Bern. A control endoscopy was performed in both groups after treatment with an elimination diet for four weeks (FRDaft) or with an elimination diet combined with prednisolone for 10 weeks (IBDaft). Intestinal control samples of gastrointestinally healthy dogs from an independent study were additionally available. Dogs were clinically examined and scored using the canine IBD activity index (CIBDAI). mRNA abundance of interleukin (IL)-5, -10, -12p40, and -13, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β1, and interferon (IFN)-γ were analyzed in intestinal samples by reverse transcription and real time polymerase chain reaction. Median CIBDAI decreased in FRDaft (P < 0.01) and IBDaft (P = 0.07) during treatment. In duodenum, IL-12p40 mRNA levels tended to be lower in FRDbef than in IBDbef (P = 0.07). The abundance of TNF-α mRNA was higher in IBDbef than in control dogs (P < 0.05). IL-5 mRNA levels decreased in FRD dogs during treatment (P = 0.06), and IL-10 mRNA levels decreased in IBD dogs (P < 0.05). In colon, IL-5 and IL-12p40 mRNA levels were lower in FRDbef than in IBDbef (P < 0.05) and control dogs (P < 0.01). IL-13 mRNA abundance was lower in FRDbef than in control dogs (P < 0.05) and IFN-γ mRNA abundance was lower in FRD and IBD dogs than in control dogs (P < 0.01). Feeding the elimination diet additionally reduced IFN-γ mRNA levels (P < 0.01), but increased TNF-α mRNA levels (P < 0.05) in FRD dogs. In conclusion, mRNA levels of the selected cytokines before treatment did not show clear differences between FRD and IBD dogs.

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“…This may have resulted from subtle differences between the biopsy specimens because they were taken from close, but not identical lesions. Moreover, it has been reported that interobserver variation and the quality of endoscopic biopsy specimens influences histopathological outcome [19,20]. Since the endoscopic biopsy specimens were taken by several operators and evaluated by several pathologists, this may have influenced the histopathological diagnoses obtained in the present study.…”

Section: Discussionmentioning

confidence: 65%

“…Lymphocyticplasmacytic inflammation can occur adjacent to or distant from the primary tumor, and distinguishing between alimentary lymphoma and enteritis, especially lymphocytic-plasmacytic enteritis (LPE), can be difficult [4,18]. Additionally, interobserver variation is also an issue in relation to histopathologic evaluation of intestinal tissues [19]. Therefore, full thickness biopsy of the intestine is helpful in making an accurate diagnosis; however, dogs that are suspected of having alimentary lymphoma are usually in poor condition and are unlikely to survive a long period of anesthesia, and laparotomic damage may also occur in some cases.…”

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confidence: 99%

Application of Polymerase Chain Reaction to Analysis of Antigen Receptor Rearrangements to Support Endoscopic Diagnosis of Canine Alimentary Lymphoma

Kaneko

Yamamoto

Wada

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. We evaluated the usefulness of polymerase chain reaction for antigen receptor gene rearrangement analysis (PARR) of endoscopic biopsy specimens for diagnosis of canine alimentary lymphoma. Two endoscopic biopsy specimens were obtained from each lesion in 78 dogs with gastrointestinal symptoms. One specimen was histopathologically examined by a pathologist, and the other was analyzed by PARR. All samples were categorized into three groups [lymphoma (n=4), adenocarcinoma (n=5) and enteritis groups (n=69)] based on the histopathological diagnosis. In the lymphoma group, one case was IgH major-positive, and three cases were TCR-positive, representing clonal expansion of B-and T-cells, respectively. PARR produced negative results for all cases in the adenocarcinoma group. In the enteritis group, six cases were TCR-positive. Two of the six TCR-positive enteritis cases were cytologically diagnosed as lymphoma by fine needle aspiration during a laparotomy. In the enteritis group, the survival times were compared between the TCR-positive and TCR-negative cases. The overall survival time of the TCR-positive enteritis cases was significantly shorter than that of the TCR-negative enteritis cases according to a log-rank test (p<0.0001). With regard to other factors, such as age, clinical signs and the serum albumin concentration, there were no significant differences between the TCR-positive and TCR-negative enteritis cases. In conclusion, PARR is capable of detecting alimentary lymphoma and latent alimentary lymphoma, which cannot be histopathologically diagnosed using endoscopic biopsy specimens. Furthermore, a TCR-positive result in PARR may imply a poor prognosis.

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Interobserver variation among histopathologic evaluations of intestinal tissues from dogs and cats

Cited by 143 publications

References 21 publications

Sensitivity for the Detection of a Clonally Rearranged Antigen Receptor Gene in Endoscopically Obtained Biopsy Specimens from Canine Alimentary Lymphoma

Sensitivity for the Detection of a Clonally Rearranged Antigen Receptor Gene in Endoscopically Obtained Biopsy Specimens from Canine Alimentary Lymphoma

Cytokine mRNA abundance in intestinal biopsies from dogs with chronic diarrhea

Application of Polymerase Chain Reaction to Analysis of Antigen Receptor Rearrangements to Support Endoscopic Diagnosis of Canine Alimentary Lymphoma

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