Internodal soluble invertase isoenzymes in relation to sucrose storage and maturity behaviour in sugarcane cultivars

Batta, S. K.; Benkush,; Sital, J. S.; Mann, Anita

doi:10.1007/bf02956912

Cited by 7 publications

(6 citation statements)

References 19 publications

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“…Pre-harvest foliar application of Zn++ (@ 1000 mg/l) showed appreciable improvement in juice quality in stale cane. Inhibitory effect of Mn ++ on cane invertases has also been reported ( Solomon et al,1990;Batta et al,2007) .…”

Section: (A)mentioning

confidence: 82%

Post-harvest deterioration of sugarcane

Solomon

2009

Sugar Tech

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Sugarcane is a perishable commodity and must be processed into sugar quickly after it is harvested. Post harvest sucrose losses have been reported from many cane producing countries and linked with low sugar recovery and several problems during sugar processing. Bio deterioration is associated with the inordinate delays between harvest to milling of sugarcane and aggravated by many intrinsic and extrinsic factors causing enormous depreciation in cane tonnage as well as sugar recovery. Besides harvest-to-mill delays, other factors such as ambient temperature, humidity, cane variety, period of storage, activities of invertases, maturity status etc. are responsible for decline in sugar recovery. The activity of invertases and proliferation of acid, ethanol and polysaccharides (dextran) producing microbes play a crucial role in the loss of recoverable sugars in cane and milled juice. In addition to loss in sugar recovery, its adverse affects has been noticed in the sugar manufacturing process and sucrose quality. Efforts have been made to reduce loss in tonnage and sucrose using physico-chemical methods. These include spraying of water, bactericidal solution, use of anti-inversion and anti-bacterial formulations and pre-harvest foliar and soil application of zinc and mangnous compounds. An integrated mill sanitation program and simultaneous use of dextranase could further improve sugar recovery and minimize problems caused by dextran. The possibility of electrolyzed water (EW) fogging to reduce post harvest deterioration in field and mill yard has also been explored. Some of these methods are useful and present larger options for the industry to minimize afterharvest quality losses in the field and milling tandem.

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Section: (A)mentioning

confidence: 82%

Post-harvest deterioration of sugarcane

Solomon

2009

Sugar Tech

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“…However, in ratoon crop one acid invertase isoform with purification fold of 36 and two neutral invertase isoforms namely N-I and N-II with purification folds of 30 and 35, respectively were obtained at this stage of cane development (Table 4). Different forms of soluble acid and neutral invertases in early (Co89003) and late (Co1148) maturing sugarcane genotypes at internodal elongation and maturity stages of crop development have earlier been reported (Batta et al 2007a). In their studies also no neutral invertase isoform was detected at stem elongation stage.…”

Section: Partial Purification and Identification Of Invertase Isoenzymesmentioning

confidence: 94%

“…From the pooled test extract, ethanol was removed at 40°C under diminished pressure to get aqueous syrup. From the clarified test extract, sucrose content was estimated, after destruction of free fructose with 3% KOH, by the method of Roe (1934) with some modifications as suggested by Batta et al (2007a). The content of reducing sugars i.e.…”

Section: Extraction and Estimation Of Sugarsmentioning

confidence: 99%

Sucrose Accumulation and Internodal Soluble Invertase Isoenzymes in Plant and Ratoon Crops of Sugarcane

et al. 2011

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Accumulation pattern of sucrose and hexoses in relation to activities of invertases in stem tissue of plant and ratoon crops of CoJ88 (mid maturing variety) has been investigated at different physiological stages of cane growth. At each stage of crop development, sucrose content was relatively more in ratoon crop than plant crop, whereas the reducing sugar content was relatively less in ratoon crop than plant crop. The activity of soluble acid invertase in stem was found to be highest at stem elongation stage and declined thereafter in both plant and ratoon crops. The activity of soluble acid invertase (pH 5.5) was markedly lower in ratoon crop as compared to plant crop at each stage of crop development. Higher level of sucrose and lower level of acid invertase in ratoon crop indicates higher sink strength and better juice quality of ratoon crop. The activity of neutral invertase (pH 7.0) increased with the advancement of the cane growth in both the crops. Three isoenzymes of soluble acid invertase in plant crop namely A-I, A-II and A-III and two isoforms of this enzyme in ratoon crop namely A-I and A-II were identified at stem elongation stage. Neutral invertase isoenzyme was not detected in both the crops at this stage. At maturation stage, one acid invertase isoform i.e. A-I was obtained in both the crops along with four neutral invertase isoenzymes in plant crop i.e. N-I, N-II, N-III and N-IV and two isoforms of this enzyme in ratoon crop i.e. N-I and N-II. These invertase isoforms differed with respect to their K m and V max values.From the temperature effect on K m and V max values, the energy of activation (E a ) and enthalpy change (DH) of invertase isoenzymes were calculated at stem elongation and maturity stages and compared among isoenzymes of plant and ratoon crops in relation to sucrose accumulation.

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“…Contrary to stem elongation stage, at ripening stage, sucrose contents of the progenies were not intermediate between those of the parents. Co 89003 has been shown to be superior in sucrose accumulation when many other sugarcane genotypes were tested (Sachdeva et al 2003;Batta et al 2007a). At maturity stage of cane development, sucrose content varied from 104.3 (Sel 25/08) to 160.6 mg g -1 fr.…”

Section: Accumulation Pattern Of Sucrose and Hexoses In Stemmentioning

confidence: 99%

Variability in Activities of Sucrose Metabolizing Enzymes in Relation to Sucrose Accumulation Among Parents and Their Progenies of Sugarcane

et al. 2011

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Variability studies on sucrose metabolizing enzymes in relation to sucrose accumulation have been done in stem tissues of parents and a set of ten progenies of the cross Co 89003 (early maturing) 9 Co Se 92423 (late maturing) during sugarcane development. Sucrose content increased from stem elongation stage to maturity stage in both parents and progenies. Sucrose concentrations of the progenies were intermediate between those of the parents at stem elongation stage. Reducing sugars content, i.e., glucose plus fructose continuously decreased with advancement of the growth of the stem in all the genotypes. Specific activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS) peaked at ripening stage and declined thereafter in each of the parents and their progenies. Specific activity of soluble acid invertase (SAI) in stem was found highest at stem elongation stage and declined thereafter in all the genotypes. Unlike SAI, soluble neutral invertase (SNI) activity increased with cane maturation. No significant overall relationship was found between the specific activities of SS/SPS/SNI and sucrose accumulation. However, a significant nonlinear negative relationship (R 2 = 0.643) between SAI and sucrose accumulation did exist over all genotypes. The difference in specific activities of SPS and SAI (SPS-SAI) and sucrose concentration were significantly positively correlated, showing a near hyperbolic relationship (R 2 = 0.495). Hence, this study may assist the sugarcane breeders in the selection of genotypes having higher capacity for sucrose accumulation and their maturity behavior under subtropical conditions.

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Internodal soluble invertase isoenzymes in relation to sucrose storage and maturity behaviour in sugarcane cultivars

Cited by 7 publications

References 19 publications

Post-harvest deterioration of sugarcane

Post-harvest deterioration of sugarcane

Sucrose Accumulation and Internodal Soluble Invertase Isoenzymes in Plant and Ratoon Crops of Sugarcane

Variability in Activities of Sucrose Metabolizing Enzymes in Relation to Sucrose Accumulation Among Parents and Their Progenies of Sugarcane

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