Interneuron synaptopathy in developing rat cortex induced by the pro-inflammatory cytokine LIF

Engelhardt, Maren; Hamad, Mohammad I. K.; Jack, Alexander; Ahmed, Küpra; König, Jennifer; Rennau, Lisa Marie; Jamann, Nora; Räk, Andrea; Schönfelder, Sabine; Riedel, Christian; Wirth, Markus Joseph; Patz, Silke; Wahle, Petra

doi:10.1016/j.expneurol.2017.12.011

Cited by 13 publications

(20 citation statements)

References 79 publications

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“…We have previously reported that GluN1, GluN2A, and GluN2B are expressed in OTC at DIV 5, DIV 10, and DIV 15-20 and the expression level of the three proteins increases with age (Engelhardt et al, 2018;Neumann et al, 2019). First, we confirmed that young neurons in OTC have functional NMDARs on the cell surface.…”

Section: Blocking Of Glun2b But Not Glun2a Prevents Nmda-induced Dendsupporting

confidence: 81%

“…Lysates and blots were performed as described (Engelhardt et al, 2018;Neumann et al, 2019) with OTC from 3 preparations that were exposed to ifenprodil, and allowed to recover from ifenprodil treatment for 12h and 24h. Using membrane strips placed over the molecular weight range of the target protein of interest several proteins of distinct kDa can be assessed per lysate.…”

Section: Protein Blotsmentioning

confidence: 99%

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GluN2B but Not GluN2A for Basal Dendritic Growth of Cortical Pyramidal Neurons

et al. 2020

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Section: Blocking Of Glun2b But Not Glun2a Prevents Nmda-induced Dendsupporting

confidence: 81%

Section: Protein Blotsmentioning

confidence: 99%

GluN2B but Not GluN2A for Basal Dendritic Growth of Cortical Pyramidal Neurons

et al. 2020

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“…Organotypic (roller-tube) cortical cultures (OTCs) were prepared from newborn P0/P1 pigmented Long Evans rats from the in-house breeding facility with approval from the Ruhr-University Animal Research Board and the State of Northrhine-Westfalia as described [28, 29, 30]. Briefly, visual cortex was explanted at postnatal day (P) 0 (day of birth) or P1, and cut into 350 μm thick slices using a McIlwain tissue chopper (Ted Pella, Redding, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Control cultures were always handled at the same time and vehicle-stimulated with the vehicle, 5 mM Tris-HCl pH 7.4. At DIV 5, 10 and 20, the cultures were fixed and processed for EGFP or GABA immunohistochemistry as described [29]. Single pulse stimulation has been done to analyse acute effects on kinase activation and phosphorylation with 1 μM Y-P30 supplemented to the medium at DIV 5, DIV 10, and DIV 20 for 15 min and for 60 min.…”

Section: Methodsmentioning

confidence: 99%

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The primate-specific peptide Y-P30 regulates morphological maturation of neocortical dendritic spines

et al. 2019

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The 30-amino acid peptide Y-P30 corresponds to the N-terminus of the primate-specific, sweat gland-derived dermcidin prepropeptide. Previous work has revealed that Y-P30 enhances the interaction of pleiotrophin and syndecans-2/3, and thus represents a natural ligand to study this signaling pathway. In immature neurons, Y-P30 activates the c-Src and p42/44 ERK kinase pathway, increases the amount of F-actin in axonal growth cones, and promotes neuronal survival, cell migration and axonal elongation. The action of Y-P30 on axonal growth requires syndecan-3 and heparan sulfate side chains. Whether Y-P30 has the potential to influence dendrites and dendritic protrusions has not been explored. The latter is suggested by the observations that syndecan-2 expression increases during postnatal development, that syndecan-2 becomes enriched in dendritic spines, and that overexpression of syndecan-2 in immature neurons results in a premature morphological maturation of dendritic spines. Here, analysing rat cortical pyramidal and non-pyramidal neurons in organotypic cultures, we show that Y-P30 does not alter the development of the dendritic arborization patterns. However, Y-P30 treatment decreases the density of apical, but not basal dendritic protrusions at the expense of the filopodia. Analysis of spine morphology revealed an unchanged mushroom/stubby-to-thin spine ratio and a shortening of the longest decile of dendritic protrusions. Whole-cell recordings from cortical principal neurons in dissociated cultures grown in the presence of Y-P30 demonstrated a decrease in the frequency of glutamatergic mEPSCs. Despite these differences in protrusion morphology and synaptic transmission, the latter likely attributable to presynaptic effects, calcium event rate and amplitude recorded in pyramidal neurons in organotypic cultures were not altered by Y-P30 treatment. Together, our data suggest that Y-P30 has the capacity to decelerate spinogenesis and to promote morphological, but not synaptic, maturation of dendritic protrusions.

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Development of myelin in fetal and postnatal neocortex of the pig, the European wild boar Sus scrofa

et al. 2023

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Myelination of the neocortex of altricial species is mostly a postnatal event, and the appearance of myelin has been associated with the end of the critical period for ocular dominance plasticity in rodent visual cortex. Due to their precocality, ungulates may tell a different story. Here, we analyzed the development of PDGFRα positive oligodendrocyte precursor cells and expression of myelin proteins in the laminar compartments of fetal and postnatal porcine cortex from E45 onwards. Precursor cell density initially increased and then decreased but remained present at P90. MAG and MBP staining were detectable at E70 in subventricular zone and deep white matter, ascending into gyral white matter at E85, and into the gray matter and marginal zone at E100 (birth in pig at E114). Protein blots confirmed the declining expression of PDGFRα from E65 onwards, and the increase of MBP and MAG expression from E80 onwards. Somatosensory input elicited by spontaneous activity is considered important for the formation of the body representation. Indeed, PDGFRα, MBP and MAG expression started earlier in somatosensory than in visual cortex. Taken together, myelination proceeded in white and gray matter and marginal zone of pig cortex before birth with an areal-specific time course, and an almost mature pattern was present at P5 in visual cortex.

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Interneuron synaptopathy in developing rat cortex induced by the pro-inflammatory cytokine LIF

Cited by 13 publications

References 79 publications

GluN2B but Not GluN2A for Basal Dendritic Growth of Cortical Pyramidal Neurons

GluN2B but Not GluN2A for Basal Dendritic Growth of Cortical Pyramidal Neurons

The primate-specific peptide Y-P30 regulates morphological maturation of neocortical dendritic spines

Development of myelin in fetal and postnatal neocortex of the pig, the European wild boar Sus scrofa

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