International Society for Extracellular Vesicles: first annual meeting, April 17–21, 2012: ISEV‐2012

Abstract

Extracellular micro- and nano-scale membrane vesicles produced by different cells are recognised as an essential entity of physiological fluids in a variety of organisms and function as mediators of intercellular communication employed for the regulation of multiple systemic and local processes. In the last decade, an exponential amount of experimental work was dedicated to exploring the biogenesis and secretion mechanisms, physiological and pathological functions and potential applications of the extracellular vesicles (EVs). Noteworthy is the large heterogeneity of in vitro and in vivo models applied, technical approaches developed in these studies and the diversity of designations assigned to different or similar types of EVs. Hence, there is a clear necessity for a uniform nomenclature and standardisation of methods to isolate and characterise these vesicles. In April 2012, the first meeting of the International Society for Extracellular Vesicles (ISEV) took place bringing together this exponentially grown scientific community. The University of Gothenburg (Krefting Research Centre) together with the Interim Board of the Society created in September 2011 (Jan Lötvall, Clotilde Théry, Xandra Breakefield, Marca Wauben, Yong Song Gho, Lawrence Rajendran, Graça Raposo, Douglas Taylor, Margareta Sjöstrand and Esbjörn Telemo) organised this fantastic event that counted 488 registered and contributing participants. This meeting report provides a retrospective summary of the broad spectrum of ISEV-2012 sessions. Again, we emphasise novel findings, discussions and decisions met by the community during the meeting.

MicroRNAs as pharmacological targets in endothelial cell function and dysfunction

“…Although the classification of extracellular vesicles is still a matter of debate, it is well accepted to distinguish them based on the cellular process that they originate from[201]. …”

Advances in Analysis of Biodistribution of Exosomes by Molecular Imaging

“…In 2007, the transfer of genetic materials such as mRNAs and miRNAs in exosomes was reported [5]. Research on exosomes has been increased explosively since then, with more than 3000 papers published annually in 2018 and 2019 ( Figure 1) [1,2,[4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22]. Exosomes, ranging 100-200 nm, from stem cells were reported to mediate the paracrine therapeutic effects of stem cells [6].…”

Detection of Aggregation-Competent Tau in Neuron-Derived Extracellular Vesicles

“…EVs larger than ~350–400 nm are thought to include plasma membrane fragments released during apoptosis, and, therefore, some have suggested it is more appropriate to describe MVs as ranging from ~50 to 400 nm [ 27 ]. Whatever the precise range of MVs, the size and composition of MVs and exosomes often overlap, and currently there are no means to completely separate or biochemically distinguish exosomes and MVs [ 28 , 29 , 30 , 31 ].…”