International consensus statement regarding the use of animal models for research on anastomoses in the lower gastrointestinal tract

Abstract

PurposeThis project aimed to reach consensus on the most appropriate animal models and outcome measures in research on anastomoses in the lower gastrointestinal tract (GIT). The physiology of anastomotic healing remains an important research topic in gastrointestinal surgery. Recent results from experimental studies are limited with regard to comparability and clinical translation.MethodsPubMed and EMBASE were searched for experimental studies investigating anastomotic healing in the lower GIT published between January 1, 2000 and December 31, 2014 to assess currently used models. All corresponding authors were invited for a Delphi-based analysis that consisted of two online survey rounds followed by a final online recommendation survey to reach consensus on the discussed topics.ResultsTwo hundred seventy-seven original articles were retrieved and 167 articles were included in the systematic review. Mice, rats, rabbits, pigs, and dogs are currently being used as animal models, with a large variety in surgical techniques and outcome measures. Forty-four corresponding authors participated in the Delphi analysis. In the first two rounds, 39/44 and 35/39 participants completed the survey. In the final meeting, 35 experts reached consensus on 76/122 items in six categories. Mouse, rat, and pig are considered appropriate animal models; rabbit and dog should be abandoned in research regarding bowel anastomoses. ARRIVE guidelines should be followed more strictly.ConclusionsConsensus was reached on several recommendations for the use of animal models and outcome measurements in research on anastomoses of the lower GIT. Future research should take these suggestions into account to facilitate comparison and clinical translation of results.Electronic supplementary materialThe online version of this article (doi:10.1007/s00384-016-2550-5) contains supplementary material, which is available to authorized users.

Enhanced anastomotic healing by Daikenchuto (TJ-100) in rats

“…In addition to the variety of animal models, a wide range of endpoints are used, although the most frequently used surrogate marker of AL in animal models is the bursting pressure of the anastomotic site. Notably, an international consensus statement was reached on several recommendations for the use of animal models for research on AL in the lower gastrointestinal tract 40 .…”

Фактори Ризику Неспроможності Колоректальних Анастомозів При Обтураційній Непрохідності (Огляд Літератури)

“…Частота виникнення НША має поліетіо ло гічну природу та залежить від рівня та умов його накладання [3,17,21,22,23,51]. Загальними факторами ризику НША є сис темні порушення макроорганізму, які здатні порушити перебіг репара тивних процесів в ділянці анастомозу [27,29,69].…”

A novel powered circular stapler designed for creating secure anastomoses

“…The pig is generally accepted as the preferred large animal model for evaluation of anastomoses in the lower gastrointestinal tract [21], as it is monogastric and similar in anatomical features to humans. The in vivo procedures were reviewed and animals approved for use in the study by the Institutional Animal Care and Use Committee.…”