International consensus guidelines on anticardiolipin and anti–β<sub>2</sub>‐glycoprotein I testing: Report from the 13th International Congress on Antiphospholipid Antibodies

Lakos, Gabriella; Favaloro, Emmanuel J.; Harris, Edward N.; Meroni, Pier Luigi; Tincani, Anǵela; Wong, Richard; Pierangeli, Silvia S.

doi:10.1002/art.33349

Cited by 175 publications

(172 citation statements)

References 61 publications

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“…5,6 Recently, a detailed protocol for the performance of aCL enzyme-linked immunosorbent assays (ELISA) 7 and an international consensus guideline on aCL and ab2GPI were published. 8 …”

Section: Introductionmentioning

confidence: 99%

Standardization of antiphospholipid antibody assays. Where do we stand?

Devreese

2012

Lupus

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The laboratory criteria (lupus anticoagulants (LA), and/or anti-cardiolipin (aCL) antibodies and/or anti-b2-glycoprotein I antibodies (ab2GPI)) that define patients with antiphospholipid syndrome (APS) were set in the Sapporo and Sydney criteria published in 1999 and 2006, respectively, and led to a substantial improvement in the recognition of APS. In addition, guidelines for LA detection were published by the Scientific Standardisation Subcommittee (SSC) of the International Society of Thrombosis and Haemostasis (ISTH) in 2009. However, a number of questions on this respect remain unresolved. Recommendations for the aCL and ab2GPI assays intended to ameliorate the performance of these solid-phase assays. Despite efforts over the years, standardization has not been reached. This review will focus on methodological issues of the three antiphospholipid antibody (aPL) subtypes that are the subject of debate. The use of an international standard for aPL detection might help solve many of the problems caused by a lack of standardization of these assays. Lupus (2012) 21, 718-721.

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“…5,6 Recently, a detailed protocol for the performance of aCL enzyme-linked immunosorbent assays (ELISA) 7 and an international consensus guideline on aCL and ab2GPI were published. 8 …”

Section: Introductionmentioning

confidence: 99%

Standardization of antiphospholipid antibody assays. Where do we stand?

Devreese

2012

Lupus

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“…При отрицательных результатах определения ВА, IgG/IgM АКЛ, IgG/IgM аβ 2 -ГПI и подозрении на наличие АФС рекомендовано дополнительное исследование АКЛ и аβ 2 -ГПI класса IgA (С) [29].…”

Section: рекомендуется однократное определение ми-озит-специфических unclassified

“…В ряде случаев обнаружение этих АФЛ ассоциирует-ся с «пре-АФС» (или «вероятным» АФС), который характеризуется наличием у больных сетчатого ли-ведо, хореи, тромбоцитопении, потерь плода, по-ражения клапанов сердца и может предшествовать развитию тромботических осложнений (С) [4,28] 8. Рекомендуемая кратность определения ВА, IgG/IgM АКЛ, IgG/IgM аβ 2 -ГПI при АФС составля-ет 1 раз в 3-6 мес (D) [28,29].…”

Section: рекомендуется однократное определение ми-озит-специфических unclassified

Laboratory diagnosis of rheumatic diseases

Александрова¹,

Новиков²,

Насонов³

2015

Lab. sluzh.

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“…The aCL and anti-β2GPI ELISAs were performed according to the current recommendations [2] and [11]. Anti-β2GPI were detected by ELISA as described previously [12], using irradiated microtitre plates (Nunc Maxisorp, Denmark).…”

Section: Other Aplmentioning

confidence: 99%

Validation of a commercially available kit to detect anti-phosphatidylserine/prothrombin antibodies in a cohort of systemic lupus erythematosus patients

Sciascia

Sanna

Murru

et al. 2014

Thrombosis Research

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Background Antiprothrombin antibodies detection comprises two different ELISAs: prothrombin coated on irradiated plates (aPT) or phosphatidylserine/prothrombin (aPS/PT) as the antigen. While several commercial kits are available for the detection of aPT, aPS/PT are usually detected by in-house assays. Recently, a new commercially available kit was launched and, therefore, we decided to test its efficiency by comparing it to our in-house assay.

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International consensus guidelines on anticardiolipin and anti–β₂‐glycoprotein I testing: Report from the 13th International Congress on Antiphospholipid Antibodies

Cited by 175 publications

References 61 publications

Standardization of antiphospholipid antibody assays. Where do we stand?

Standardization of antiphospholipid antibody assays. Where do we stand?

Laboratory diagnosis of rheumatic diseases

Validation of a commercially available kit to detect anti-phosphatidylserine/prothrombin antibodies in a cohort of systemic lupus erythematosus patients

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International consensus guidelines on anticardiolipin and anti–β2‐glycoprotein I testing: Report from the 13th International Congress on Antiphospholipid Antibodies

Cited by 175 publications

References 61 publications

Standardization of antiphospholipid antibody assays. Where do we stand?

Standardization of antiphospholipid antibody assays. Where do we stand?

Laboratory diagnosis of rheumatic diseases

Validation of a commercially available kit to detect anti-phosphatidylserine/prothrombin antibodies in a cohort of systemic lupus erythematosus patients

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Product

Resources

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International consensus guidelines on anticardiolipin and anti–β₂‐glycoprotein I testing: Report from the 13th International Congress on Antiphospholipid Antibodies