International collaborative study by in vitro bioassays of the first International Standard for porcine inhibin

Das, Rose E. Gaines; Rose, Matthew P.; Zanelli, Joan M.

doi:10.1530/jrf.0.0960803

Cited by 11 publications

(4 citation statements)

References 13 publications

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“…Because the GnSIF was purified from pig follicular fluid and an anti-human inhibin antibody was used, both pig and human inhibin were used in the bioassay. There is considerable cross-species conservation of the inhibin molecule and both pig and human inhibin have biological effects on the secretion of FSH by rat pituitary cells (Gaines Das et al, 1992;Rivier et al, 1991). The First International Standard preparation for pig inhibin (NIBSC 86/690) was used in some of the experiments and in others an NIBSC-ampouled preparation of recombinant DNA derived human inhibin was used.…”

Section: Discussionmentioning

confidence: 99%

“…Two different preparations of inhibin (pig and human) were used because the anti-inhibin antibody was raised against human inhibin and the preparation of GnSIF was purified from pig follicular fluid. The pig preparation was the First International Standard for Porcine Inhibin (Code 86/690) (Gaines Das et al, 1992) in doses of 0.2, 2.0, 6.25, 12.5, 20, 25, 50, 100 and 200 iu ml~. The second preparation was recombinantly derived 32 kDa human inhibin A, which was an NIBSC ampouled preparation, with a nominal content of 5 pg per ampoule.…”

Section: Inhibin Dose-responsementioning

confidence: 99%

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In vitro bioactivity of gonadotrophin surge attenuating factor is not affected by an antibody to human inhibin

Balen¹,

Er²,

Rafferty³

et al. 1995

Reproduction

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The effect of anti-inhibin antibodies on gonadotrophin surge attenuating/inhibiting factor (GnSAF/GnSIF) and its effect on gonadotrophin secretion in a pituitary cell bioassay were determined by culturing rat pituitary cells in a serum-free medium to which inhibin and a partially purified preparation of gonadotrophin surge inhibiting factor (GnSIF) were added. The samples were treated with an anti-inhibin antiserum and basal and GnRH-stimulated FSH and LH secretion were measured by radioimmunoassay of the culture supernatant. Inhibin had a dose-dependent inhibitory effect on both basal and GnRH-stimulated FSH secretion and also on GnRH-stimulated LH secretion. Anti-inhibin antibody blocked the inhibition of FSH secretion, except at the highest two doses of human inhibin (1.25 ng ml-1, P = 0.04; 2.5 ng ml-1, P = 0.0004). The GnSIF preparation inhibited basal and GnRH-stimulated FSH and GnRH-stimulated LH secretion and was not affected by the anti-inhibin antibody. The novel hormone, GnSIF, is different from inhibin.

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Section: Discussionmentioning

confidence: 99%

Section: Inhibin Dose-responsementioning

confidence: 99%

In vitro bioactivity of gonadotrophin surge attenuating factor is not affected by an antibody to human inhibin

Balen¹,

Er²,

Rafferty³

et al. 1995

Reproduction

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“…Concentrations of these steroids in the charcoal-treated PS were all bellow the sensitivity of RIA. One ml of the PFF preparation represented approximately 400,000 IU of WHO International Inhibin Standard [14], as assessed by the rat anterior pituitary cell bioassay system [13].…”

Section: Porcine Follicular Fluid (Pff) and Porcine Serum (Ps)mentioning

confidence: 99%

Roles of the Basal Level of LH and FSH in the Regulation of Follicular Development during Pseudopregnancy in the Rat.

Kishi

Kondoh

Nagamine

et al. 1997

Journal of Reproduction and Development

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Abstract. Present study was conducted to investigate roles of the basal level of FSH and LH in the follicular maturation and maintenance during pseudopregnancy of the rat. Antiserum against luteinizing hormone releasing hormone (LHRH-AS) or charcoal-treated porcine follicular fluid (PFF; a crude inhibin preparation) was given to individual animals on day 5 of pseudopregnancy. Plasma LH and inhibin decreased after treatment with LHRH-AS. Contrarily, plasma FSH increased after LHRH-AS injection. The number of follicles capable of ovulating in response to human chorionic gonadotropin (hCG) in animals given LHRH-AS declined gradually, reached a nedir at 24 h after the treatment, and then returned to control levels during the subsequent 24 h. On the other hand, plasma FSH levels in rats given three injections of 0.5 ml PFF at 6-h intervals, compared to those in control animals, remained significantly lower at nearly all time points during the first 24 h after the initial PFF injection, and thereafter the hormone levels temporarily increased, being significantly elevated above the control value at 30 and 42 h after the initial PFF injection. Only in the rats given intraperitoneal injections of 0.5 ml PFF, compared to the control animals, plasma LH concentrations remained significantly lower at 9 h after the initial PFF injection and those values were significantly higher at 36 h after the initial treatment. The number of follicles capable of ovulating in response to hCG decreased in PFF-treated animals in a dose-dependent-manner. The follicular development in animals given injection of 0.5 ml PFF were virtually or completely inhibited from 12 to 36 h after the initial injection of PFF. Present results clearly indicate that the basal secretion of both LH and FSH are essential for follicular development and maintenance during pseudopregnancy of the rat. These results also suggest that inhibin is a primary factor to suppress FSH secretion in the pseudopregnant rat. Key words: Pseudopregnant rat, Inhibin, Follicles, FSH, LH.(J. Reprod. Dev. 43: [279][280][281][282][283][284][285][286][287] 1997) ntral follicles usually exist in the ovary during pseudopregnancy of the rat, although plasma reported previously [4,5]. A specific surge of FSH is not essential for the recruitment and development of a new set of follicles in cyclic rats [6]. On the other hand, there is a previous study that shows the importance of basal levels of FSH in plasma to cause a normal follicular development and maturation in cyclic rats [7]. It has been known that both LH and FSH are necessary to maintain the activity of growing follicles [8,9]. Administration of LH caused a follicular A concentrations of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were suppressed to the basal level [1][2][3]. Plasma concentrations of inhibin and estradiol showed relatively high basal levels during this period as

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“…Estradiol, progesterone and testosterone levels in the charcoal-treated PFF were 38.0 pg/ml, 4.0 ng/ml and 40.6 pg/ml respectively [10]. One ml of PFF preparation contained inhibin equivalent to approximately 400,000 IU of WHO International Standard inhibin [11], as assessed by the rat anterior pituitary cell bioassay system [12]. Porcine serum was also treated with dextran-coated charcoal and served as a control fluid for PFF.…”

Section: Porcine Follicular Fluid (Pff) and Porcine Serum (Ps)mentioning

confidence: 99%

Involvement of Ovarian Inhibin and Steroid Hormones in the Regulation of Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH) Secretion during Pseudopregnancy in the Rat.

Kishi

Kondo

Arai

et al. 1997

Journal of Reproduction and Development

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Abstract. Roles of ovarian inhibin and steroids in the regulation of basal secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) were examined during pseudopregnancy in the rat. Bilateral or unilateral ovariectomy performed on either day 5 or 10 of pseudopregnancy (day 0; the day after copulation with a vasectomized male) produced a significant increase in plasma FSH concentrations within 6 h. During the subsequent 18 h, plasma FSH concentrations in unilaterally ovariectomized rats declined to a baseline level, and the gonadotropin concentrations in bilaterally ovariectomized rats remained significantly elevated above the value measured in sham-operated (control) animals. Plasma LH concentrations on either day 5 or 10 were not significantly altered by bilateral or unilateral ovariectomy. Plasma inhibin concentrations on both days 5 and 10 declined to an undetectable level within 6 h after bilateral ovariectomy. In unilateral ovariectomized rats, compared to control animals, a significant decrease in plasma inhibin concentrations was evident at 6 and 12 h, but not at 24 h, after the operation on both days 5 and 10. On day 5, administration of charcoal-treated porcine follicular fluid (an inhibin preparation) eliminated the increase in plasma FSH concentrations produced by bilateral ovariectomy within 24 h without affecting plasma LH concentrations. Plasma FSH and LH concentrations in rats bilaterally ovariectomized on day 5 were not significantly altered by administration of estradiol or progesterone alone, whereas both gonadotropin concentrations in these animals were slightly but significantly suppressed as late as 24 h after combined administration of estradiol and progesterone. In addition, in intact pseudopregnant rats, administration of antiserum against inhibin resulted in a marked increase in plasma FSH concentrations accompanied by a small but significant increase in plasma LH on day 5. These results collectively suggest that ovarian inhibin is a major regulator of FSH secretion during pseudopregnancy in the rat.

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International collaborative study by in vitro bioassays of the first International Standard for porcine inhibin

Cited by 11 publications

References 13 publications

In vitro bioactivity of gonadotrophin surge attenuating factor is not affected by an antibody to human inhibin

In vitro bioactivity of gonadotrophin surge attenuating factor is not affected by an antibody to human inhibin

Roles of the Basal Level of LH and FSH in the Regulation of Follicular Development during Pseudopregnancy in the Rat.

Involvement of Ovarian Inhibin and Steroid Hormones in the Regulation of Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH) Secretion during Pseudopregnancy in the Rat.

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