Internalization studies on zeolite nanoparticles using human cells

Vilaça, Natália; Totovao, Ricardo; Prasetyanto, Eko Adi; Miranda-Gonçalves, Vera; Morais-Santos, Filipa; Fernandes, Rui; Figueiredo, Francisco; Bañobre‐López, Manuel; Fonseca, A.; Cola, Luisa De; Baltazar, Fátima; Neves, Isabel C.

doi:10.1039/c7tb02534c

Cited by 11 publications

(7 citation statements)

References 49 publications

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“…All these structures present high thermal and chemical stability and are capable of introducing more than one metal by ion exchange, in order to compensate the negative charge of the framework. 21,22,27,28 Although NaY and NaA show a higher cation-exchange capacity due to their Si/Al ratio (NaY, Si/Al = 2.40, used in this study and 2.83; 22 NaA, Si/Al = 1.24, 41 compared to ZSM-5, Si/Al = 15.0), 42 the location of the cations is dependent on the charge-compensating cations in the structures, which confer the antimicrobial behavior to the samples (Figure 1). 21,22,41−43 Therefore, the preparation of the metal ion−zeolite materials was obtained by two-step insertion of the metal ions in liquid phase: (i) introduction of the first metal ion into the zeolite structure followed by (ii) the insertion of the second metal ion.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The substitution of Al 3+ for Si 4+ in TO 4 induces a negative charge in the zeolite structure, which is balanced by the exchangeable cation to preserve the electroneutrality of the solid, resulting in a higher cation-exchange capacity. Zeolites can be natural or synthetic, the latter having more advantages than the natural ones due to higher purity, a more uniform pore size, and better ion-exchange abilities. , All these properties make zeolites suitable for different applications in catalysis, drug delivery systems, adsorption, ion exchange, and separation. − These structures have also been used as antimicrobial agents upon introduction of metal ions with antimicrobial properties by the ion-exchange method, − namely, silver, − ,,,,, zinc, ,,− and copper. ,,, .…”

Section: Introductionmentioning

confidence: 99%

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Metal Ion–Zeolite Materials against Resistant Bacteria, MRSA

Peixoto

Guedes

Rombi

et al. 2021

Ind. Eng. Chem. Res.

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Metal ion–zeolite materials based on different zeolite structuresFAU (NaY, 100–750 nm), LTA (4400 nm), and MFI (ZSM5, >100, 760, and 2500 nm) were studied for developing antimicrobial agents using a simple method. These zeolite structures were loaded with silver, copper, or zinc ions using an ion-exchange method and tested for antimicrobial activity against three bacteriaEscherichia coli, methicillin-sensitive Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus (MRSA), a clinical isolate. Sample characterization confirmed the presence of metal ions and the stability of the zeolite structures. All mono- and bimetallic ion–zeolite materials loaded with silver displayed higher antimicrobial activity than zinc- or copper-containing samples. The inhibitory effect of metal ion–zeolite materials was even higher than the action of antibiotics, especially against MRSA. These findings show that silver ion–zeolite materials have potential applications to combat bacterial infections and further prevent the prevalence of resistant microorganisms. The antimicrobial activity seems to be related to the acidity and Si/Al ratio of zeolites, according to the following scale: MFI > FAU > LTA. Particularly, the inhibitory effect against MRSA is a very promising result considering the difficulty of effective eradication of this resistant strain, the principal agent of nosocomial or healthcare-associated infections, which affect 3.2% of all hospitalized patients in the United States and 6.5% in the European Union.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metal Ion–Zeolite Materials against Resistant Bacteria, MRSA

Peixoto

Guedes

Rombi

et al. 2021

Ind. Eng. Chem. Res.

Self Cite

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“…Free Cy5 and Cy5 loaded NPs without membrane coating (Cy5-N) and Cy5 loaded membrane coated NPs (Cy5-MN) (each containing the same content of Cy5) were added into the medium of BMDM and Caco-2 cells with the final concentration of Cy5 as 10 μg/mL and the concentration of NPs as 0.19 mg/mL. The internalization of free Cy5 and Cy5 loaded NPs was determined by flow cytometry in 4 hours and 24 hours, respectively according to the previous studies 30 , 31 and the mean fluorescence intensity was statistically analyzed.…”

Section: Methodsmentioning

confidence: 99%

Amelioration of ulcerative colitis via inflammatory regulation by macrophage-biomimetic nanomedicine

et al. 2020

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Ulcerative colitis (UC) is featured with relapsing inflammation in the colon, where macrophages are recruited and polarized locally into M1 type to drive further inflammation. Pharmacotherapy of UC has exhibited limited efficacy, mostly due to the poor specificity. Methods: A macrophage-biomimetic nanomedicine was developed for targeted treatment of UC, which was derived from reactive oxygen species (ROS)-sensitive β-cyclodextrin, loaded with rosiglitazone, and coated with macrophage membrane. The ability of the nanomedicine in regulating macrophage polarization was examined at cellular level, and the macrophage-tropism driven targeted delivery into the inflammatory colon was investigated by ex vivo bio-imaging distribution assay. Furthermore, the nanomedicine's therapeutic efficacy was systemically examined in dextran sulfate sodium (DSS)-induced colitis model in mice. Results: The nanomedicine effectively polarized macrophages to M2 and protected epithelial cells from oxidative stress in vitro . In addition, macrophage-membrane led the nanomedicine to the inflammatory colon with a high targeting efficiency. In response to the elevated ROS in the inflammatory tissue, the nanomedicine released rosiglitazone specifically and regulated macrophage polarization in vivo . Macrophage membrane also assisted inflammation suppression by sequestering proinflammatory cytokines. Working in such a synergy, the nanomedicine exhibited significant therapeutic effects against UC in mice. Conclusions: This macrophage-biomimetic nanomedicine leverages the inflammatory tropism and inflammatory cytokine sequestration effects of macrophage membrane for targeted delivery and local inflammation suppression, the ROS-responsiveness of β-cyclodextrin-based matrix for specific payload release, and the macrophage-polarizing effect of rosiglitazone for inflammatory regulation, thereby exhibiting considerable therapeutic efficacy against UC in mice. This study offers important new insights on the design and development of biomimetic nanomaterials for inflammation regulations.

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“…43 The current results are also in an agreement with other studies reported that nanoparticles with a size of 50 nm and 120 nm are internalized faster in comparison to 250 nm particles. 44 In a very recent study, 45 the kinetics of uptake of zeolite L and NaY by Hs 578T breast cancer cells and MCF-10 epithelial mammary cells was presented. The NaY zeolite had cubic geometry with an average diameter of 700 nm, while zeolite L had disc-shaped particles with a size of around 400 nm.…”

Section: Nanosized Ru(bpy)3-fau Zeolite As An Intracellular Localization Tracermentioning

confidence: 99%

Ruthenium tris(2,2′-bipyridyl) complex encapsulated in nanosized faujasite zeolite as intracellular localization tracer

Komaty

Özçelik

Zaarour

et al. 2021

Journal of Colloid and Interface Science

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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Internalization studies on zeolite nanoparticles using human cells

Abstract: Internalization of zeolite L is a caveolin-mediated process, which is higher and faster in breast cancer cells than in normal cells.

Cited by 11 publications

References 49 publications

Metal Ion–Zeolite Materials against Resistant Bacteria, MRSA

Metal Ion–Zeolite Materials against Resistant Bacteria, MRSA

Amelioration of ulcerative colitis via inflammatory regulation by macrophage-biomimetic nanomedicine

Ruthenium tris(2,2′-bipyridyl) complex encapsulated in nanosized faujasite zeolite as intracellular localization tracer

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