Internalization of mesoporous silica nanoparticles induces transient but not sufficient osteogenic signals in human mesenchymal stem cells

Dong, Hao; Chung, Tsai Hua; Hung, Yu-Chueh; Lü, Fang; Wu, Si Han; Mou, Chung‐Yuan; Yao, Ming; Chen, Yao Chang

doi:10.1016/j.taap.2008.04.009

Cited by 124 publications

(72 citation statements)

References 35 publications

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“…64,65 Previous studies have shown that internalized silica nanoparticles have no apparent deleterious effects on the morphology, proliferation, viability, and differentiation efficiency of mesenchymal stem cells. 66,67 The findings from our study suggest that biologically prepared AgNPs seem to be less toxic than AgNO 3 at tested concentrations in F9 cells. Therefore, we selected AgNPs for further analysis to demonstrate the effect of AgNPs on differentiation of F9 cells.…”

Section: Agnps Induce the Expression Of Differentiation Markers And Ementioning

confidence: 67%

Dual functions of silver nanoparticles in F9 teratocarcinoma stem cells, a suitable model for evaluating cytotoxicity- and differentiation-mediated cancer therapy

Han

Gurunathan

Choi

et al. 2017

IJN

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Background Silver nanoparticles (AgNPs) exhibit strong antibacterial and anticancer activity owing to their large surface-to-volume ratios and crystallographic surface structure. Owing to their various applications, understanding the mechanisms of action, biological interactions, potential toxicity, and beneficial effects of AgNPs is important. Here, we investigated the toxicity and differentiation-inducing effects of AgNPs in teratocarcinoma stem cells. Materials and methods AgNPs were synthesized and characterized using various analytical techniques such as UV–visible spectroscopy, X-ray diffraction, energy-dispersive X-ray spectroscopy, and transmission electron microscopy. The cellular responses of AgNPs were analyzed by a series of cellular and biochemical assays. Gene and protein expressions were analyzed by reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. Results The AgNPs showed typical crystalline structures and spherical shapes (average size =20 nm). High concentration of AgNPs induced cytotoxicity in a dose-dependent manner by increasing lactate dehydrogenase leakage and reactive oxygen species. Furthermore, AgNPs caused mitochondrial dysfunction, DNA fragmentation, increased expression of apoptotic genes, and decreased expression of antiapoptotic genes. Lower concentrations of AgNPs induced neuronal differentiation by increasing the expression of differentiation markers and decreasing the expression of stem cell markers. Cisplatin reduced the viability of F9 cells that underwent AgNPs-induced differentiation. Conclusion The results showed that AgNPs caused differentially regulated cytotoxicity and induced neuronal differentiation of F9 cells in a concentration-dependent manner. Therefore, AgNPs can be used for differentiation therapy, along with chemotherapeutic agents, for improving cancer treatment by targeting specific chemotherapy-resistant cells within a tumor. Furthermore, understanding the molecular mechanisms of apoptosis and differentiation in stem cells could also help in developing new strategies for cancer stem cell (CSC) therapies. The findings of this study could significantly contribute to the nanomedicine because this study is the first of its kind, and our results will lead to new strategies for cancer and CSC therapies.

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Section: Agnps Induce the Expression Of Differentiation Markers And Ementioning

confidence: 67%

Dual functions of silver nanoparticles in F9 teratocarcinoma stem cells, a suitable model for evaluating cytotoxicity- and differentiation-mediated cancer therapy

Han

Gurunathan

Choi

et al. 2017

IJN

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“…[4][5][6][7] The biosafety of nanoparticles (NPs) is a prerequisite for their application in biomedicine. 9 One of the major challenges in theranostic applications of SPIONs is to improve their selectivity and efficacy and reduce their side effects. 5 There is accumulated evidence that metallic NPs have cytotoxic potential due to an enhanced reactive surface area and the ability to cross cell and tissue barriers.…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles

Pongrac¹,

Pavičić²,

Milić³

et al. 2016

IJN

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Biocompatibility, safety, and risk assessments of superparamagnetic iron oxide nanoparticles (SPIONs) are of the highest priority in researching their application in biomedicine. One improvement in the biological properties of SPIONs may be achieved by different functionalization and surface modifications. This study aims to investigate how a different surface functionalization of SPIONs – uncoated, coated with d -mannose, or coated with poly- l -lysine – affects biocompatibility. We sought to investigate murine neural stem cells (NSCs) as important model system for regenerative medicine. To reveal the possible mechanism of toxicity of SPIONs on NSCs, levels of reactive oxygen species, intracellular glutathione, mitochondrial membrane potential, cell-membrane potential, DNA damage, and activities of SOD and GPx were examined. Even in cases where reactive oxygen species levels were significantly lowered in NSCs exposed to SPIONs, we found depleted intracellular glutathione levels, altered activities of SOD and GPx, hyperpolarization of the mitochondrial membrane, dissipated cell-membrane potential, and increased DNA damage, irrespective of the surface coating applied for SPION stabilization. Although surface coating should prevent the toxic effects of SPIONs, our results showed that all of the tested SPION types affected the NSCs similarly, indicating that mitochondrial homeostasis is their major cellular target. Despite the claimed biomedical benefits of SPIONs, the refined determination of their effects on various cellular functions presented in this work highlights the need for further safety evaluations. This investigation helps to fill the knowledge gaps on the criteria that should be considered in evaluating the biocompatibility and safety of novel nanoparticles.

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“…Several in vitro studies have demonstrated the feasibility of modifying stem cells with nanoparticles that include dendrimers. [135][136][137][138] Stem cells that have been modified to promote differentiation that is individually tailored for the type of injury has the potential to aid treatment of older patients with cerebral palsy.…”

Section: Drug1mentioning

confidence: 99%

Nanomedicine in cerebral palsy

Balakrishnan¹,

Nance²,

Johnston³

et al. 2013

IJN

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Internalization of mesoporous silica nanoparticles induces transient but not sufficient osteogenic signals in human mesenchymal stem cells

Cited by 124 publications

References 35 publications

Dual functions of silver nanoparticles in F9 teratocarcinoma stem cells, a suitable model for evaluating cytotoxicity- and differentiation-mediated cancer therapy

Dual functions of silver nanoparticles in F9 teratocarcinoma stem cells, a suitable model for evaluating cytotoxicity- and differentiation-mediated cancer therapy

Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles

Nanomedicine in cerebral palsy

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