Internal Ribosome Entry Site Structural Motifs Conserved among Mammalian Fibroblast Growth Factor 1 Alternatively Spliced mRNAs

Martineau, Yvan; Bec, Christine Le; Monbrun, Laurent; Allo, Valérie; Chiu, Ing‐Ming; Danos, Olivier; Moine, Hervé; Piégay, Hervé; Prats, Anne-Catherine

doi:10.1128/mcb.24.17.7622-7635.2004

Cited by 59 publications

(59 citation statements)

References 53 publications

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“…Furthermore, our previous study has shown a high conservation of the FGF-1 IRES A secondary structure and function in mammals. 14 Our data suggest that the FGF-1 IRES is muscle specific. The FGF-1 IRES activity would require specific IRES trans-acting factors (ITAFs) to work efficiently, as shown for other cellular IRESs.…”

Section: Discussionmentioning

confidence: 62%

“…As observed previously with plasmids transfected in the same cell line, the activity of the FGF-1 IRES was about three to four times lower to that of the EMCV IRES (Figure 1c). 14,15 C2C12 mouse myoblasts were transduced by the same virions. Transduction was less efficient than with 911 cells: an MOI of 10 5 was necessary to obtain a detectable luciferase expression (Figure 2a).…”

Section: Resultsmentioning

confidence: 99%

“…15 In addition, measurements of IRES activities after plasmid electrotransfer into mouse skeletal muscle have revealed that the IRES from the fibroblast growth factor 1 (FGF-1) mRNA leader A is as active as the EMCV IRES. 14 Furthermore, in a tricistronic context, the FGF-2 IRES induces the expression of exogenous cDNA in human melanoma cells without any positive or negative regulation from the other IRES present in the vector, in contrast to EMCV or HTLV-1 IRES. 16 The use of IRESs for an optimized gene transfer seems particularly adequate in conditions of stress.…”

Section: Introductionmentioning

confidence: 99%

“…The FGF-1 IRES, which can be reduced to a size of about 100 nt, is interesting in this respect, especially for a use in viral vectors where the size of the transgene expression cassette is limited. 14 Adeno-associated virus (AAV) derived vectors are highly effective for stable gene transfer in a number of tissues, including skeletal muscle, liver, retina and brain where long-lasting expression of therapeutic transgenes have been obtained in pre-clinical and human clinical studies. 19,20 AAV particles have a low packaging capacity and the size of sequences inserted into the vectors is strictly limited to less than 5 kb.…”

Section: Introductionmentioning

confidence: 99%

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Efficient gene transfer in skeletal muscle with AAV-derived bicistronic vector using the FGF-1 IRES

et al. 2008

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Section: Discussionmentioning

confidence: 62%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Efficient gene transfer in skeletal muscle with AAV-derived bicistronic vector using the FGF-1 IRES

et al. 2008

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“…IRES-containing mRNAs mostly encode regulatory proteins such as, e.g., growth factors and transcription factors. Several studies have reported that under stress conditions, where cap-dependent translation is blocked, translation of specific mRNAs is enabled through IRES elements ( [270] and references therein). Another function of IRESs involves the control of alternative initiation of translation.…”

Section: Important Regulatory Motifs In Utrs Of Mrnasmentioning

confidence: 99%

Evolutionary patterns of non-coding RNAs

Bompfünewerer

Flamm

Fried

et al. 2005

Theory in Biosciences

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A plethora of new functions of non-coding RNAs have been discovered in past few years. In fact, RNA is emerging as the central player in cellular regulation, taking on active roles in multiple regulatory layers from transcription, RNA maturation, and Manuscript January 2005RNA modification to translational regulation. Nevertheless, very little is known about the evolution of this "Modern RNA World" and its components. In this contribution we attempt to provide at least a cursory overview of the diversity of non-coding RNAs and functional RNA motifs in non-translated regions of regular messenger RNAs (mRNAs) with an emphasis on evolutionary questions. This survey is complemented by an in-depth analysis of examples from different classes of RNAs focusing mostly on their evolution in the vertebrate lineage. We present a survey of Y RNA genes in vertebrates, studies of the molecular evolution of the U7 snRNA, the snoRNAs E1/U17, E2, and E3, the Y RNA family, the let-7 microRNA family, and the mRNA-like evf-1 gene. We furthermore discuss the statistical distribution of microRNAs in metazoans, which suggests an explosive increase in the microRNA repertoire in vertebrates. The analysis of the transcription of non-coding RNAs (ncRNAs) suggests that small RNAs in general are genetically mobile in the sense that their association with a hostgene (e.g. when transcribed from introns of a mRNA) can change on evolutionary time scales. The let-7 family demonstrates, that even the mode of transcription (as intron or as exon) can change among paralogous ncRNA.

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Emerging role of angiogenin in stress response and cell survival under adverse conditions

2012

Journal Cellular Physiology

145

127

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Angiogenin (ANG), also known as ribonuclease (RNASE) 5, is a member of the vertebrate-specific, secreted RNASE superfamily. ANG was originally identified as a tumor angiogenic factor, but its biological activity has been extended from inducing angiogenesis to stimulating cell proliferation and more recently, to promoting cell survival. Under growth conditions, ANG is translocated to nucleus where it accumulates in nucleolus and stimulates ribosomal RNA (rRNA) transcription, thus facilitating cell growth and proliferation. Under stress conditions, ANG is accumulated in cytoplasmic compartments and modulates the production of tiRNA, a novel class of small RNA that is derived from tRNA and is induced by stress. tiRNA suppress global protein translation by inhibiting both cap-dependent and -independent translation including that mediated by weak IRESes. However, strong IRES-mediated translation, a mechanism often used by genes involved in pro-survival and anti-apoptosis, is not affected. Thus, ANG-mediated tiRNA reprogram protein translation, save anabolic energy, and promote cell survival. This recently uncovered function of ANG presents a novel mechanism of action in regulating cell growth and survival.

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Internal Ribosome Entry Site Structural Motifs Conserved among Mammalian Fibroblast Growth Factor 1 Alternatively Spliced mRNAs

Cited by 59 publications

References 53 publications

Efficient gene transfer in skeletal muscle with AAV-derived bicistronic vector using the FGF-1 IRES

Efficient gene transfer in skeletal muscle with AAV-derived bicistronic vector using the FGF-1 IRES

Evolutionary patterns of non-coding RNAs

Emerging role of angiogenin in stress response and cell survival under adverse conditions

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