Intermolecular complexes between three human CD1 molecules on normal thymus cells

Amiot, Martine; Dastot, Hélène; Fabbi, Marina; Degos, Laurent; Bernard, Alain; Boumsell, Laurence

doi:10.1007/bf00346585

Cited by 21 publications

(8 citation statements)

References 40 publications

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“…Many examples of alterna-bulin.Thus, CDla is found covalently attached to CD8 by a disulfide bond, and this interaction appears to exclude PI-microglobulin [29,301. In immunoprecipitation from iodinated MOLT4 membranes, CDlb and CDlc are found associated with additional proteins of 200 and 16 kDa, respectively [31], and it has also been recently claimed that CDla can bind to CDlb, CDlc and MHC class I molecules non-covalently in the thymus [32]. Is there any evidence that similar interactions exist when CD1 is expressed in the mouse thymus ?…”

Section: Discussionmentioning

confidence: 98%

Expression of CD1 in the mouse thymus

1990

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The CD1 antigens are a family of differentiation antigens found predominantly, but not exclusively, in the human thymus. Although three antigens (CD1a-c) are described by monoclonal antibodies, five genes (CD1A-E) are found in the human genome. The cloning of the mouse CD1 genes (Bradbury, A., Belt, K.T., Nery, T.M., Milstein, C. and Calabi, F., EMBO J. 1988. 7:3081) demonstrated the presence of homologues to human CD1D, but not to any of the other human CD1 genes. In this work we have examined the expression of mouse CD1D mRNA in the thymus and shown that it is predominantly cortical, as is the expression of the CD1 antigens in man. Somewhat surprisingly, we also find that most CD1D mRNA in the mouse thymus is unspliced. Despite this, we have also been able to show, using a polyclonal antiserum directed against a bacterial fusion protein, the existence of the expected protein product.

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Section: Discussionmentioning

confidence: 98%

Expression of CD1 in the mouse thymus

1990

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“…Whether these constraints involve intramolecular interactions not present in MHC class I molecules or involve CD1 associations with molecules other than p2-microglobulin remains to be determined. In addition to an association between CD1 and CD8 (13,14), there is evidence suggesting that other cell surface molecules may interact with CD1 (30)(31)(32). These associations may be important clues in understanding the function of this gene family.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of a cDNA and gene coding for a fourth CD1 molecule.

Balk

Bleicher

Terhorst

1989

Proc. Natl. Acad. Sci. U.S.A.

104

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The CD1 locus encodes a family of major histocompatibility complex (MHC) antigen-like glycoproteins which associate with 182-microglobulin and are expressed on immature thymocytes and Langerhans cells. Three CD1 molecules have been identified by monoclonal antibodies and molecular cloning: CDla, -b, and -c. We have isolated a cDNA coding for a fourth CD1 molecule from a human thymocyte library and termed this molecule CD1d. Reported here are the complete nucleotide sequence and genomic organization of CD1d. They predict that this molecule is related to the previously identified CDla, -b, and -c molecules and to MHC class I molecules, with three external domains, a transmembrane domain, and a short cytoplasmic tail. The sequence of CD1d is the most divergent among the CD1 molecules in the membrane-distal al and a2 domains and in the 5' untranslated region. In contrast, all four CD1 molecules are highly homologous in the membrane-proximal a3 domain, which is likely involved in 82-microglobulin binding. A comparison of CD1 and MHC class I sequences suggests that these molecules each evolved to interact with a distinct set of cell surface proteins.

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“…Unlike MHC molecules that display allelic polymorphism, CD1 molecules display little or no polymorphism between individuals of an outbred population (5). This conservation in CD1 structure suggests that CD1 has evolved to present Ags of limited structural variability (4).…”

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confidence: 99%

Group 1 CD1 Genes in Rabbit

Hayes

Knight

2001

The Journal of Immunology

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CD1 is an Ag-presenting molecule that can present lipids and glycolipids to T cells. The CD1 genes were first identified in the human, and since then, homologs have been identified in every mammalian species examined to date. Over a decade ago, CD1B and CD1D homologs were identified in the rabbit. We have extended this earlier study by identifying additional CD1 genes with the goal of developing the rabbit as an animal model to study the function of CD1 proteins. We constructed a thymocyte cDNA library and screened the library with CD1-specific probes. Based on nucleotide sequence analyses of the CD1+ cDNA clones obtained from the library, we have identified two CD1A genes and one CD1E gene as well as determined the complete sequence of the previously identified CD1B gene. The CD1E+ cDNA clones lacked the transmembrane and cytoplasmic domains and, if translated, would encode for a soluble or secreted CD1E protein. In addition, expression studies demonstrated that the CD1 genes were expressed in peripheral lymphoid tissues as well as in skin, gut, and lung. Of interest is the finding that CD1A2, CD1B, and CD1E genes were found to be expressed by rabbit B cell populations. The rabbit, with a complex CD1 locus composed of at least two CD1A genes, one CD1B gene, one CD1D gene, and one CD1E gene, is an excellent candidate as an animal model to study CD1 proteins.

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Intermolecular complexes between three human CD1 molecules on normal thymus cells

Cited by 21 publications

References 40 publications

Expression of CD1 in the mouse thymus

Expression of CD1 in the mouse thymus

Isolation and characterization of a cDNA and gene coding for a fourth CD1 molecule.

Group 1 CD1 Genes in Rabbit

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