Intermittent theta burst stimulation ameliorates cognitive impairment and hippocampal gliosis in the Streptozotocin-induced model of Alzheimer's disease

Stanojevic, Jelená́; Dragic, Milorad; Stevanović, Ivana; Ilić, Tihomir V.; Stojanović, Ivana; Zeljković, Milica; Ninković, Milica

doi:10.1016/j.bbr.2022.113984

Cited by 10 publications

(14 citation statements)

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“…Furthermore, it has been shown that rTMS may improve mitochondrial viability ( Medina-Fernandez et al, 2017 ), which is a significant source of reactive oxygen species, and attenuate pro-apoptotic cascade improving cellular viability in general ( Uzair et al, 2022 ). Interestingly, both BDNF and Nrf2 are involved in synaptic plasticity, learning, and memory which are impaired in STZ-induced neurotoxicity ( Singh and Garabadu, 2021 ; Tiwari et al, 2021 ; Stanojevic et al, 2022 ). Given that we previously showed improvement in learning and memory after iTBS, these results might provide additional rationale for the observed improvement ( Stanojevic et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…The animals underwent stereotaxic surgical procedures as previously explained ( Stanojevic et al, 2022 ). Briefly, all animals were anesthetized with an intraperitoneal injection (ip) of Ketamidor (50 mg/kg body weight (bw); Richter Pharma AG, Austria) and Xylased 2% (5 mg/kg bw; Bioveta, Czech Republic).…”

Section: Methodsmentioning

confidence: 99%

“…Theta burst stimulation (TBS) was applied in the form of iTBS, applying the protocol according to Huang et al (2007) , using a MagStim Rapid2 device and a 25-mm figure-of-eight coil (The MagStim Company, Whitland, Dyfed, United Kingdom) as previously described ( Stanojevic et al, 2022 ). Briefly, the iTBS block was consisting of 20 trains of 10 bursts (3 pulses at a frequency of 50 Hz), repeated at 5 Hz (with 10 s intervals between trains; total duration 192 s; schematically presented in Figure 1 ), so each block contained a total of 600 pulses.…”

Section: Methodsmentioning

confidence: 99%

“…The brains were carefully removed on ice, fixed in 4% paraformaldehyde (PFA) in 0.1 M PBS (pH 7.4, 24 h at 4°C), cryoprotected and dehydrated in graded sucrose (10%–30% in 0.2 M phosphate buffer; 4°C) and sectioned on the cryotome (Leica CM1850, Germany), as described before ( Stanojevic et al, 2022 ). Afterward, 25 μm thick coronal cryosections of the dorsal hippocampus, prefrontal cortex (PFC), and caudoputamen (CPu) were collected serially, mounted on superfrost glass slides, air-dried for 2 h at room temperature (RT), and stored at −20°C until further use.…”

Section: Methodsmentioning

confidence: 99%

“…A protocol known as intermittent theta burst stimulation (iTBS) is a form of rTMS that requires less stimulation time, and lower intensity, and induces a long-lasting positive effect in the human cerebral cortex ( Huang et al, 2005 ). Recent studies showed the positive effects of iTBS on cognitive performance in both AD patients ( Golaszewski et al, 2021 ; Wu et al, 2022 ) and animal models ( Stanojevic et al, 2022 ). Also, rTMS restored the disturbed balance between parameters of antioxidant protection and oxidative stress in experimental rats ( Liang et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Intermittent theta burst stimulation attenuates oxidative stress and reactive astrogliosis in the streptozotocin-induced model of Alzheimer’s disease-like pathology

Stanojevic

Zeljković

Dragic

et al. 2023

Front. Aging Neurosci.

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IntroductionIntracerebroventricularly (icv) injected streptozotocin (STZ) is a widely used model for sporadic Alzheimer’s disease (sAD)-like pathology, marked by oxidative stress-mediated pathological progression. Intermittent theta burst stimulation (iTBS) is a noninvasive technique for brain activity stimulation with the ability to induce long-term potentiation-like plasticity and represents a promising treatment for several neurological diseases, including AD. The present study aims to investigate the effect of the iTBS protocol on the animal model of STZ-induced sAD-like pathology in the context of antioxidant, anti-inflammatory, and anti-amyloidogenic effects in the cortex, striatum, hippocampus, and cerebellum.MethodsMale Wistar rats were divided into four experimental groups: control (icv normal saline solution), STZ (icv STZ—3 mg/kg), STZ + iTBS (STZ rats subjected to iTBS protocol), and STZ + Placebo (STZ animals subjected to placebo iTBS noise artifact). Biochemical assays and immunofluorescence microscopy were used to evaluate functional and structural changes.ResultsThe icv STZ administration induces oxidative stress and attenuates antioxidative capacity in all examined brain regions. iTBS treatment significantly reduced oxidative and nitrosative stress parameters. Also, iTBS decreased Aβ-1-42 and APP levels. The iTBS enhances antioxidative capacity reported as elevated activity of its enzymatic and non-enzymatic components. In addition, iTBS elevated BDNF expression and attenuated STZ-induced astrogliosis confirmed by decreased GFAP+/VIM+/C3+ cell reactivity in the hippocampus.DiscussionOur results provide experimental evidence for the beneficial effects of the applied iTBS protocol in attenuating oxidative stress, increasing antioxidant capacity and decreasing reactive astrogliosis in STZ-administrated rats.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Intermittent theta burst stimulation attenuates oxidative stress and reactive astrogliosis in the streptozotocin-induced model of Alzheimer’s disease-like pathology

Stanojevic

Zeljković

Dragic

et al. 2023

Front. Aging Neurosci.

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The effect of intermittent theta burst stimulation for cognitive dysfunction: a meta-analysis

Zheng,

Chen,

Cao

et al. 2024

Brain Injury

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Experimental Design and Behavioral Testing Protocol for the Evaluation of Cognitive Abilities and Social Behavior in Mice Following Early Life Stress

Khafizova,

Naumova,

Lopez

et al. 2024

Современная Зарубежная Психология

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<p style="text-align: justify;">This manuscript presents a protocol designed for the comprehensive investigation of early life stress (ELS) outcomes and a feasibility study conducted with this protocol. ELS alters normal development by interfering at various levels: hormonal changes, brain cellular architecture, epigenome, and chromosomal structural elements. The protocol combines classic behavioral tests with advanced molecular techniques to obtain comprehensive data and thus uncover the underlying mechanisms of ELS. In this protocol, the main source of stress is maternal separation. Briefly, a group of C57Bl/6 mice undergoes maternal separation; then, mice perform the radial maze test and the resident-intruder test. As a control, another group of mice stays undisturbed and performs the same behavioral tests in the same timeframe. After the behavioral tests, biosamples are collected, including urine for corticosterone measurements, peripheral blood, hippocampus, amygdala, and prefrontal cortex tissues for DNA isolation and its downstream analyses (DNA methylation profiling and telomere length measuring), and whole brains for immunohistochemistry analysis of the glucocorticoid receptor density. This protocol was successfully tested as a feasibility study for a large-scale investigation that addresses potential flaws to establish a robust methodology. This paper reports on a comprehensive approach to examining multiple aspects of development that interrogates a holistic analysis of multilayer and multidimensional data and may contribute valuable insights for both animal and human studies.</p>

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Intermittent theta burst stimulation ameliorates cognitive impairment and hippocampal gliosis in the Streptozotocin-induced model of Alzheimer's disease

Cited by 10 publications

References 44 publications

Intermittent theta burst stimulation attenuates oxidative stress and reactive astrogliosis in the streptozotocin-induced model of Alzheimer’s disease-like pathology

Intermittent theta burst stimulation attenuates oxidative stress and reactive astrogliosis in the streptozotocin-induced model of Alzheimer’s disease-like pathology

The effect of intermittent theta burst stimulation for cognitive dysfunction: a meta-analysis

Experimental Design and Behavioral Testing Protocol for the Evaluation of Cognitive Abilities and Social Behavior in Mice Following Early Life Stress

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