Intermittent fasting promotes anxiolytic-like effects unrelated to synaptic mitochondrial function and BDNF support

Carteri, Randhall Bruce; Menegassi, Lizia Nardi; Feldmann, Marceli; Kopczynski, Afonso; Rodolphi, Marcelo Salimen; Strogulski, Nathan Ryzewski; Almeida, Amanda Staldoni; Marques, Daniela Melo; Porciúncula, Lisiane O.; Portela, Luis Valmor

doi:10.1016/j.bbr.2021.113163

Cited by 9 publications

(3 citation statements)

References 76 publications

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“…after 3 weeks on IF [44]. Hazzaa et al (2020) also reported increased distance traveled during OF using male Wistar rats fed a HFD in an alternate day fasting pattern.…”

Section: Plos Onementioning

confidence: 85%

Comparison of intermittent fasting and voluntary wheel running on physical and cognitive abilities in high-fat diet-induced obese rats

Gopalan,

Niepoetter,

Butts-Wilmsmeyer

et al. 2023

PLoS ONE

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Regular physical activity is a proven routine for weight management in addressing obesity. Another method that has gained attention for its health benefits is intermittent fasting (IF). Physical and cognitive abilities while on these routines are poorly understood in the obese population. Sixty-five male Sprague Dawley rats at 7 weeks of age were subjected to diet-induced obesity by feeding a high-fat diet (HFD) or a standard diet (SD) for 8 weeks, after which behavioral testing was performed to detect any changes in physical and cognitive abilities. Rats from the HFD-fed (now considered obese) and SD-fed groups were then subjected to IF (18-hour fast and 6-hour feeding daily), voluntary wheel running (VWR), or control conditions for 3 weeks before repeating the same behavioral testing protocol. IF resulted in less weight gain (p<0.05) and elevated ketone levels (p<0.05) in both SD and HFD-fed groups. IF improved physical activity when compared to VWR and control animals in both SD and HFD-fed groups (p<0.05) while the VWR group in the SD-fed rats exhibited less physical fatigue compared to IF and controls (p<0.05). Additionally, elevated ketone levels were weakly correlated with decreased physical (p<0.0001) and exploratory behavior (p<0.01). These results suggest that IF is more effective than VWR in HFD and SD-fed rats in minimizing weight gain and retaining physical activity, and ketones may play a part in establishing the reported physical benefits. Exploration of physiological mechanisms between ketones, diet, and exercise will help fight obesity and many associated diseases.

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“…after 3 weeks on IF [44]. Hazzaa et al (2020) also reported increased distance traveled during OF using male Wistar rats fed a HFD in an alternate day fasting pattern.…”

Section: Plos Onementioning

confidence: 85%

Comparison of intermittent fasting and voluntary wheel running on physical and cognitive abilities in high-fat diet-induced obese rats

Gopalan,

Niepoetter,

Butts-Wilmsmeyer

et al. 2023

PLoS ONE

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show abstract

“… 5 , 8 , 9 , 10 , 26 In addition, proBDNF and nerve growth factor precursor (proNGF) belong to neurotrophin precursors, and overexpression of proNGF may render lethal cardiomyopathy in adulthood. 27 Therefore, we hypothesized that McAb‐proB may attenuate the cardiac remodeling of AMI. Surprisingly, the treatment of McAb‐proB has impaired cardiac function and increased infarct area and mortality after AMI.…”

Section: Discussionmentioning

confidence: 99%

Brain‐Derived Neurotrophic Factor Precursor Contributes to a Proinflammatory Program in Monocytes/Macrophages After Acute Myocardial Infarction

Luo

et al. 2023

JAHA

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Background The imbalance of monocyte/macrophage polarization toward the preferential proinflammatory phenotype and a lack of normal inflammation resolution are present in acute myocardial infarction (AMI). Our previous study showed that upregulation of brain‐derived neurotrophic factor precursor (proBDNF) in M2‐like monocytes may contribute to the proinflammatory response in the Stanford type‐A acute aortic dissection. The present study aimed to investigate the role of proBDNF signaling in monocytes/macrophages in the progress of AMI. Methods and Results We observed the upregulation of proBDNF in the proinflammatory monocytes of patients with AMI. The upregulation of proBDNF was also observed in the circulating proinflammatory Ly6C high monocytes and cardiac F4/80 + CD86 + macrophages 3 days after AMI in a mice model. To neutralize proBDNF, the mice subjected to AMI were injected intraperitoneally with a monoclonal anti‐proBDNF antibody. Echocardiography, 2,3,5‐triphenyltetrazolium chloride staining, and positron emission tomography/computed tomography results demonstrate that monoclonal anti‐proBDNF antibody treatment further impaired cardiac functions, increased infarct size, and exacerbated the proinflammatory state. Moreover, the level of proinflammatory Ly6C high in the blood and F4/80 + CD86 + in the heart was further increased in monoclonal anti‐proBDNF antibody mice. RNA sequencing revealed that matrix metalloprotease‐9 protein level was dramatically increased, along with the activated proinflammatory‐related cytokines. Matrix metalloprotease‐9 inhibitor treatment attenuated the deteriorated effect of monoclonal anti‐proBDNF antibody on cardiac function and infarct areas. Conclusions Our study shows that endogenous proBDNF in monocytes/macrophages may exert protective roles in cardiac remodeling after AMI by regulating matrix metalloprotease‐9 activity.

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“…Research on Chaos carolinensis showed that basal respiration decreased progressively during starvation, and a spectrofluorometric assay based on the 2',7'-dichlorofluorescein diacetate dye indicated greater H 2 O 2 and reactive oxygen species (ROS) generation in starved cells than in fed cells, and fasting increased oxidative stress (Deng et al 2002 ). However, there was no difference between the intermittent fasting (IF) and control groups in isolated liver mitochondrial H 2 O 2 production in mice (Carteri et al 2021 ). Previous studies have reported inconsistent views on the redox state after fasting; in particular, redox changes at the organelle level are rarely reported.…”

Section: Introductionmentioning

confidence: 99%

Exploring the precision redox map during fasting-refeeding and satiation in C. elegans

et al. 2023

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Fasting is a popular dietary strategy because it grants numerous advantages, and redox regulation is one mechanism involved. However, the precise redox changes with respect to the redox species, organelles and tissues remain unclear, which hinders the understanding of the metabolic mechanism, and exploring the precision redox map under various dietary statuses is of great significance. Twelve redox-sensitive C. elegans strains stably expressing genetically encoded redox fluorescent probes (Hyperion sensing H2O2 and Grx1-roGFP2 sensing GSH/GSSG) in three organelles (cytoplasm, mitochondria and endoplasmic reticulum (ER)) were constructed in two tissues (body wall muscle and neurons) and were confirmed to respond to redox challenge. The H2O2 and GSSG/GSH redox changes in two tissues and three organelles were obtained by confocal microscopy during fasting, refeeding, and satiation. We found that under fasting condition, H2O2 decreased in most compartments, except for an increase in mitochondria, while GSSG/GSH increased in the cytoplasm of body muscle and the ER of neurons. After refeeding, the redox changes in H2O2 and GSSG/GSH caused by fasting were reversed in most organelles of the body wall muscle and neurons. In the satiated state, H2O2 increased markedly in the cytoplasm, mitochondria and ER of muscle and the ER of neurons, while GSSG/GSH exhibited no change in most organelles of the two tissues except for an increase in the ER of muscle. Our study systematically and precisely presents the redox characteristics under different dietary states in living animals and provides a basis for further investigating the redox mechanism in metabolism and optimizing dietary guidance.

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Intermittent fasting promotes anxiolytic-like effects unrelated to synaptic mitochondrial function and BDNF support

Cited by 9 publications

References 76 publications

Comparison of intermittent fasting and voluntary wheel running on physical and cognitive abilities in high-fat diet-induced obese rats

Comparison of intermittent fasting and voluntary wheel running on physical and cognitive abilities in high-fat diet-induced obese rats

Brain‐Derived Neurotrophic Factor Precursor Contributes to a Proinflammatory Program in Monocytes/Macrophages After Acute Myocardial Infarction

Exploring the precision redox map during fasting-refeeding and satiation in C. elegans

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