Intermittent fasting decreases oxidative stress parameters in Wistar rats (Rattus norvegicus)

Abstract

Background<br />Chronic and degenerative diseases are closely related to modern lifestyles that tend to be deficient in physical activity but excessive in food intake. One method used to overcome this problem is dietary restriction through intermittent fasting. Intermittent fasting decreases the risk of chronic and degenerative diseases, e.g. by lowering oxidative stress. Oxidative stress can be determined from the malondialdehyde (MDA) levels and lipid profile in the blood. The present study aimed to determine the effect of intermittent fasting on plasma MDA levels and lipid profile of Wistar rats (Rattus norvegicus).<br /><br />Methods<br />This research was a laboratory experimental research using a post test control group design. Twenty four male Wistar rats (Rattus norvegicus) were randomly assigned to 4 groups, ie control group (C), fasting group (F), non-fasting obese group (NFO), and fasting obese group (FO). Fasting treatment given in this research was intermittent fasting, comprising one day of fasting for 12 hours alternating with one day of normal feed ad libitum in the span of 8 weeks. After completion of treatment, blood was taken transcardially for examination of MDA levels and lipid profiles using spectrophotometry.<br /><br />Results<br />There were significant between-group differences in MDA levels and lipid profiles (p<0.05 for all parameters). Groups treated with intermittent fasting had lower levels of MDA, total cholesterol, triglycerides, and ow-density lipoprotein (LDL) than those without intermittent fasting. The high-density lipoprotein (HDL) levels were higher in the intermittent fasting group than the other groups.<br /><br />Conclusion<br />Intermittent fasting has an effect on the oxidative stress parameters of Wistar rats (Rattus norvegicus).

Time-Restricted Feeding Improved Vascular Endothelial Function in a High-Fat Diet-Induced Obesity Rat Model

“…However, TRF did not affect HDL-C levels in obese rats. These findings are similar to data reported in the previous studies [ 32 , 42 , 43 ].…”

Effect of Dawood Fasting on the Increased Level of Antioxidant Enzymes

“…The statistical analysis showed that the mean levels of SOD antioxidant in the DF group were significantly higher than those in the ALC and HFC groups. Our previous study found that DF could reduce the level of oxidative stress through the malondialdehyde (MDA) marker compared to the AL diet [21]. A previous study of IF for 24 h also indicated an increase in SOD levels and a decrease in MDA levels in the liver of rats induced with alcoholic fatty liver disease and in the kidney of DM rats compared to that of DM-induced rats with AL diet [24].…”

“…The fasting includes abstinence from eating, drinking, or smoking, and the recommendation is to start it with having a pre-dawn meal and to end it by eating iftar in the evening. Our previous study showed that although DF indicates no changes in the parameters of lipid profile, hematology [19], and quality of life among the elderly [20], there is a decrease in the oxidative stress and lipid profile among the mice given high carbohydrate and fat diets [21]. The purpose of this study is to prove the effect of DF on the changes in SOD, GPx, and Cat antioxidant enzymes in BALB/c strain mice.…”

Intermittent Fasting Ameliorated High-Fat Diet-Induced Memory Impairment in Rats via Reducing Oxidative Stress and Glial Fibrillary Acidic Protein Expression in Brain

“…Our data were in line with Marbut et al (2005) [ 43 ], as there was significant decrease in serum cholesterol, TGs and LDL levels with significant increase in HDL levels after IF for one month. Nurmasitoh et al [ 44 ] also found higher HDL levels in rats subjected to IF.…”