Intermittent administration of PTH induces the expression of osteocalcin and BMP-2 on choroid plexus cells associated with suppression of sclerostin, TGF-β1, and Na+K+ATPase

Giovanini, Allan Fernando; Göhringer, Isabella; Tavella, Rosangela; Linzmeyer, Manuelly Cristiny; Priesnitz, Thaynara Fernanda; Bonetto, Luana Mordask; Resende, Rafaela Guimarães; Scariot, Rafaela; Zielak, João César

doi:10.1007/s12020-017-1490-3

Cited by 2 publications

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“…The trend towards normalization of PTH after KT might be able to lose inhibitory effect on osteocyte cells, probably leading to an increase of bone local levels of sclerostin expression, however, we did not see clearly this finding, because remains attenuated by the use of ZA. In our patients, these findings could express the well-known negative feedback between PTH and sclerostin [ 34 , 35 ]. In vitro studies have shown the enhancing effects of glucocorticoids [ 36 ] and Scl in bone marrow adipogenesis [ 31 ].…”

Section: Discussionmentioning

confidence: 64%

The effect of vitamin D and zoledronic acid in bone marrow adiposity in kidney transplant patients: A post hoc analysis

et al. 2018

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PurposeOsteoblasts and adipocytes are derived from mesenchymal stem cells. An imbalance in the differentiation of these lineages could affect the preservation of bone integrity. Several studies have suggested the importance of this imbalance in the pathogenesis of osteoporosis after kidney transplant (KT), but the role of bone marrow adiposity in this process is not well known, and if the treatment with the anti-absorptive (zoledronic acid-ZA) drugs could attenuate bone loss. Thus, our objective was compare bone marrow adiposity, osteoblasts and osteocytes before and after KT, verify an association between bone remodeling process (Turnover, Volume, and Mineralization—TMV classification), the osteocyte sclerostin expression to evaluate if there is a role of Wnt pathway, as well as the effect of ZA on these cells.MethodsWe studied 29 new living-donor KT patients. One group received ZA at the time of KT plus cholecalciferol for twelve months, and the other group received only cholecalciferol. Bone biopsies were performed at baseline and after 12 months of treatment. Histomorphometric evaluation was performed in bone and bone marrow adipocytes. Sclerostin (Scl) expression in osteocytes was evaluated by immunohistochemistry.ResultsSome bone marrow adiposity parameters were increased before KT. After KT, some of them remained increased and they worsened with the use of ZA. In the baseline, lower bone Volume and Turnover, were associated with increased bone marrow adiposity parameters (some of them). After KT, both groups showed the same associations. Osteocyte Scl expression after KT decreased with the use of ZA. We observed also an inverse association between bone adiposity parameters and lower osteocyte sclerostin expression 12 months after KT.ConclusionIn conclusion, the present study suggests that KT fails to normalize bone marrow adiposity, and it even gets worse with the use of ZA. Moreover, bone marrow adiposity is inversely associated with bone Volume and Turnover, which seems to be accentuated by the antiresorptive therapy.

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Section: Discussionmentioning

confidence: 64%

The effect of vitamin D and zoledronic acid in bone marrow adiposity in kidney transplant patients: A post hoc analysis

et al. 2018

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“…One hypothesis that should be considered and may explain the results found here is the likely action of TGFβ1, which was previously shown to increase upon alendronate administration[ 17 ]. It is noteworthy that this cytokine is an important growth factor that may contribute to mineral expansion.…”

Section: Discussionmentioning

confidence: 87%

Dose of alendronate directly increases trabeculae expansivity without altering bone volume in rat femurs

Weiss

Kuchar

Gerber

et al. 2018

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AIMTo evaluate the effects of sodium alendronate on bone repair in fractures created in appendicular bones.METHODSWistar rats (n = 36) were allocated into three distinct groups: group C (control), group B1 (received 1 mg/kg of alendronate), and group B2 (received 3 mg/kg of alendronate). The rats underwent femoral transversal linear fracture surgery using stable internal fixation with a 2.0 mm plate and screw system. Each animal randomly received intraperitoneal applications of sodium alendronate at a dose corresponding to group B1 or B2 three times a week, while the control group received a 0.9% saline solution. Drug administration was performed until euthanasia at 45 d. The femurs were removed and each surgical piece was sent for radiographic, tomographic and microtomographic analysis. Data were submitted to descriptive and inferential statistical analysis (95% confidence interval).RESULTSQuantitative evaluations of bone neoformation did not show differences among the groups in the radiographic (P = 0.341), microtomographic (P = 0.581) and tomographic evaluations (P = 0.171). In the qualitative microtomographic analysis, a smaller distance was observed between the internal bone trabeculae in the groups that used alendronate (P = 0.05). On the other hand, group B2 had a higher amount of bone trabeculae per unit length when compared to the other groups (P = 0.04).CONCLUSIONIt is likely that the use of alendronate did not have a direct influence on the amount of bone neoformation, however it did influence the bone quality in a dose-dependent manner, ultimately affecting the distance and quantity of the trabeculae.

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Intermittent administration of PTH induces the expression of osteocalcin and BMP-2 on choroid plexus cells associated with suppression of sclerostin, TGF-β1, and Na+K+ATPase

Cited by 2 publications

References 18 publications

The effect of vitamin D and zoledronic acid in bone marrow adiposity in kidney transplant patients: A post hoc analysis

The effect of vitamin D and zoledronic acid in bone marrow adiposity in kidney transplant patients: A post hoc analysis

Dose of alendronate directly increases trabeculae expansivity without altering bone volume in rat femurs

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