Intermembrane transfer and antioxidant action of α-tocopherol in liposomes

Kagan, V. E.; Bakalova, Rumiana; Zhelev, Zh .; Rangelova, D.; Serbinova, E. A.; Tyurin, V. A.; Denisova, Natalie; Packer, Lester

doi:10.1016/0003-9861(90)90529-8

Cited by 23 publications

(6 citation statements)

References 10 publications

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“…A similar decrease compared with control was observed when ascorbate was omitted during the resealing step (results not shown). Whether ␣-tocopherol was transferred from erythrocytes to the liposomes (51,52) or oxidized by the liposomes (53) is controversial. Although lipid peroxidation was not detected in either liposomes or ghosts following incubation of intact cells with liposomes in the present studies, the failure to detect quantitative transfer supports an oxidative loss.…”

Section: Resultsmentioning

confidence: 99%

Interaction of Ascorbate and α-Tocopherol in Resealed Human Erythrocyte Ghosts

May

Morrow

1996

Journal of Biological Chemistry

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A role for ascorbate-derived electrons in protection against oxidative damage to membrane lipids was investigated in resealed human erythrocyte ghosts. Incubation of resealed ghosts with the membrane-impermeant oxidant ferricyanide doubled the ghost membrane concentration of F 2 -isoprostanes, a sensitive marker of lipid peroxidation. Incorporation of ascorbate into ghosts during resealing largely prevented F 2 -isoprostane formation due to extravesicular ferricyanide. This protection was associated with a rapid transmembrane oxidation of intravesicular ascorbate by extravesicular ferricyanide. Transmembrane electron transfer, which was measured indirectly as ascorbate-dependent ferricyanide reduction, correlated with the content of ␣-tocopherol in the ghost membrane in several respects. First, ascorbate resealed within ghosts protected against ferricyanide-induced oxidation of endogenous ␣-tocopherol in the ghost membrane. Second, when exogenous ␣-tocopherol was incorporated into the ghost membrane during the resealing step, subsequent ferricyanide reduction was enhanced. Last, incubation of intact erythrocytes with soybean phospholipid liposomes, followed by resealed ghost preparation, caused a proportional decrease in both the membrane content of ␣-tocopherol and in ferricyanide reduction. Incorporation of exogenous ␣-tocopherol during resealing of ghosts prepared from liposome-treated cells completely restored the ferricyanide-reducing capacity of the ghosts. These results suggest that the transmembrane transfer of ascorbate-derived electrons in erythrocyte ghosts is dependent in part on ␣-tocopherol and that such transfer may help to protect the erythrocyte membrane against oxidant stress originating outside the cell.

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Section: Resultsmentioning

confidence: 99%

Interaction of Ascorbate and α-Tocopherol in Resealed Human Erythrocyte Ghosts

May

Morrow

1996

Journal of Biological Chemistry

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“…Предположительно, более выраженный эффект липосом, состоящих из ЯФХ, обусловлен изменением свойств их мембранных слоев. В работе [13] было показано, что токоферол спонтанно перемещается между слоями фосфолипидов и может переходить с липосом на мембраны клеток даже без поглощения липосомных частиц. Причем такой межмембранный переход выражен в большей степени, если липосомы содержат ненасыщенные жирные кислоты.…”

Section: Generation Of Reactive Oxygen Species and The Level Of Prounclassified

Oxidative Stress Markers in the Lungs Under Experimental Hyperoxia With Inhaled Liposomes Based on Egg Lecithin

2019

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Oxidative stress is considered to be a factor leading to lung damage in premature infants. The aim of this study was to investigate the effect of inhaled antioxidants incorporated into egg lecithin liposomes on the indicators of oxidative stress in the lungs of newborn guinea pigs under experimental hyperoxia (3 days). Bronchoalveolar lavage fl uid (BALF) was used as a material for the study. Under hyperoxia exposure, inhalation of liposomes containing N-acetylcysteine and alpha-tocopherol contributed to the suppression of the reactive oxygen species production by cells, normalization of glutathione peroxidase activity and carbonyls content, while not affecting the level of diene conjugates in BALF. The introduction of retinoid-containing liposomes (retinol and retinoic acid) under hyperoxia was accompanied by normalization of glutathione peroxidase activity as well as the content of protein oxidation products in BALF, while the generation of reactive oxygen species remained enhanced, and the diene conjugates and thiobarbituric acid reactive products exceeded the levels in animals exposed to hyperoxia alone. Thus, the inhaled liposomes containing retinoids and egg lecithin exhibit not only anti-, but also a prooxidant effect in the lungs under hyperoxia exposure, unlike the liposomal forms of N-acetylcysteine and alpha-tocopherol.

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“…Tocopherols can readily be incorporated into both monolayers of unilamellar liposomes in a monomeric form (16). Furthermore, tocopherol in liposomes can undergo spontaneous intermembrane transfer to an acceptor membrane without the fusion of the tocopherol liposome (16).…”

Section: Lipid Soluble Antioxidantsmentioning

confidence: 99%

“…Furthermore, tocopherol in liposomes can undergo spontaneous intermembrane transfer to an acceptor membrane without the fusion of the tocopherol liposome (16). This intermembrane transfer is more pronounced when the tocopherol liposome contains polyunsaturated fatty acids (16). RRR-alpha-tocopherol and RRR-alphatocotrienol are forms of vitamin E that have the same aromatic chromanol head group hut differ in the structure of their hydrocarbon tails.…”

Section: Lipid Soluble Antioxidantsmentioning

confidence: 99%

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Liposome Delivery of a Potential Antidote for Mustard Exposure. An Overview and Integration of Data for Phase One Experiments by the Mustard Consortium, Regarding the Use of Antioxidants as a Counter Measure to Mustard

Hill¹

2000

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Intermembrane transfer and antioxidant action of α-tocopherol in liposomes

Cited by 23 publications

References 10 publications

Interaction of Ascorbate and α-Tocopherol in Resealed Human Erythrocyte Ghosts

Interaction of Ascorbate and α-Tocopherol in Resealed Human Erythrocyte Ghosts

Oxidative Stress Markers in the Lungs Under Experimental Hyperoxia With Inhaled Liposomes Based on Egg Lecithin

Liposome Delivery of a Potential Antidote for Mustard Exposure. An Overview and Integration of Data for Phase One Experiments by the Mustard Consortium, Regarding the Use of Antioxidants as a Counter Measure to Mustard

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