Intermediate States in the Precipitation of Hydroxyapatite

Eanes, E. D.; Gillessen, I H; Posner, Aaron S.

doi:10.1038/208365a0

Cited by 498 publications

(278 citation statements)

References 4 publications

Supporting

Mentioning

258

Contrasting

Unclassified

Order By: Relevance

“…This conclusion was drawn from the following facts: (1) when CaPO 4 are prepared by rapid precipitation from aqueous solutions containing ions of calcium and orthophosphate at pH [ 8.5, the initial solid phase is amorphous; (2) mature bone mineral is composed of a mixture of ion-substituted ACP and poorly crystallized ionsubstituted CDHA; (3) early bone mineral has a lower crystallinity than mature bone and the observed improvement in crystallinity with the age of the bone mineral is a result of a progressive reduction in the ACP content (Elliott 2002;Eanes et al 1965;Termine and Posner 1966a, b;Harper and Posner 1966;Posner 1969Posner , 1973Posner , 1978Boskey and Posner 1976;Glimcher et al 1981). Interestingly, thermodynamic data prove that the transition of freshly precipitated ACP into CDHA involves intermediate formation of OCP (Meyer and Eanes 1978a, b).…”

Section: Bonementioning

confidence: 99%

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Dorozhkin¹

2017

Morphologie

View full text Add to dashboard Cite

Section: Bonementioning

confidence: 99%

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Dorozhkin¹

2017

Morphologie

View full text Add to dashboard Cite

“…33,34 The composite polymer nanofibers were produced using a two-step process: (a) mixing Zr-ACP powder with solvent and polymer and (b) airbrushing the composite onto TCPS discs.…”

Section: Polymer Composite Nanofiber Fabricationmentioning

confidence: 99%

Airbrushed Composite Polymer Zr-ACP Nanofiber Scaffolds with Improved Cell Penetration for Bone Tissue Regeneration

Hoffman

Škrtić²,

Sun³

et al. 2015

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

Electrospun polymer nanofibers have multiple applications in the tissue engineering field despite limited cell penetration within the scaffolds and slow synthesis rates. Airbrushing, a proposed alternative to traditional electrospinning, is a technique capable of synthesizing open structure nanofiber scaffolds at high rates. In this study, three biocompatible polymers-poly-D,L-lactic acid (P-DL-LA), polycaprolactone (PCL), and poly (methyl methacrylate) (PMMA), were airbrushed to form networks for bone tissue regeneration. All three polymers were loaded with up to 20% (w/w) zirconium-modified amorphous calcium phosphate (Zr-ACP). A simple one-step mix and straightforward material deposition yielded open structure networks with welldistributed Zr-ACP. Cell penetration within the airbrushed scaffolds was found to be more than twice the cell penetration within conventional electrospun networks. The airbrushed polymer network supported cell growth and differentiation. Cells grown on the Zr-ACP in P-DL-LA fibers exhibited improved levels of osteocalcin protein with an increase in the Zr-ACP content by day 16. This airbrushing method promises to be a viable and attractive alternative to currently used electrospinning techniques in the formation of composite 3D nanofiber scaffolds for tissue engineering applications.

show abstract

“…Alternatively, the CaP-coated plates were prepared according to the following method: preincubated 0.12 M Na2HPO4 and 0.2 M CaCl2 (50 mM Tris HCl pH7.4) in a 5% CO2 incubator at 37°C were mixed, and then the CaP slurry was washed with sterile water. The concentration of calcium and phosphate under neutral pH used was ascertained to induce spontaneous precipitation of HA due to the supersaturated condition of the solution 12) . The apatite slurry was poured into wells of 24-well plates and dried at 37°C overnight.…”

Section: Calcium Phosphate (Cap)-coated Platementioning

confidence: 99%

Effect of chondroitin sulfate-E on the osteoclastic differentiation of RAW264 cells

Miyazaki

Miyauchi²,

Tawada³

et al. 2010

Dent. Mater. J.

View full text Add to dashboard Cite

The present study was designed to investigate whether chondroitin sulfate (CS)-E, a CS structural isomer variant, alter the differentiation of macrophage cell line RAW264 cells to osteoclast-like cells. CS-B, CS-E, low molecular weight CS-E, synthetic chondroitin polysulfate (CPS) and heparin significantly inhibited the formation of tartrate-resistant acid phosphatase-positive multinuclear cells and pit formation on calcium phosphate (CaP)-coated plates. CS-E pre-coated on the CaP plate also inhibited pit formation. Digestion of CS on the cell surface by chondroitinase showed no effect on the osteoclastic differentiation of RAW264 cells whereas inhibitory effect on the differentiation of osteoblastic cell line MC3T3-E1. On the other hand, exogenously added fluoresceinlabeled CS-E directory bound to fibronectin and RAW264 cells. These results suggest that CS-E structure on the surface of osteoblasts or bone matrix binds to cell adhesion molecule such as integrin on the pre-osteoclastic cells and inhibits the differentiation into osteoclasts. CS-E may have a potential in treating bone defect if combined with CaP materials.

show abstract

Intermediate States in the Precipitation of Hydroxyapatite

Cited by 498 publications

References 4 publications

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Airbrushed Composite Polymer Zr-ACP Nanofiber Scaffolds with Improved Cell Penetration for Bone Tissue Regeneration

Effect of chondroitin sulfate-E on the osteoclastic differentiation of RAW264 cells

Contact Info

Product

Resources

About