Intermediate Filament Expression in Mouse Embryonic Stem Cells and Early Embryos

Xue, Zhigang; Moura‐Neto, Vivaldo; Izmiryan, Araksya; Martins, Sheila Cristina de Souza; Larcher, Jean Christophe; Paulin, Denise; Li, Zhenlin

doi:10.1007/978-90-481-3375-8_5

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“…We demonstrated here that synemin expression is characteristic of mouse ES cell lines in the undifferentiated state and the neural stem cells at both mRNA and protein levels. Our experiment showed that mouse pluripotent ES cells expressed synemin M, prior to nestin, which is considered as a marker of neural stem cells [ 20 , 29 , 30 ]. Moreover, this expression was associated with a common transcriptional signature that is shared by stem cells.…”

Section: Discussionmentioning

confidence: 99%

“…We asked if synemin is also expressed in mouse embryonic stem (ES) cells, which can differentiate into a variety of somatic cell types including lineages from three embryonic germ layers? The presence and function of IF proteins in ES cells are not yet fully understood, only a few members of this family have been studied [ 17 - 20 ]. The mRNAs coding for keratins 7, 8, 18 and 19 are present in the 2-cell stage embryo, but only K7 and K8 (type II) are translated into protein in 4- to 8-cell stage embryos at a low level, which is deposited in aggregates [ 18 , 19 , 21 - 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Dynamic expression of synemin isoforms in mouse embryonic stem cells and neural derivatives

et al. 2011

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BackgroundIntermediate filaments (IFs) are major components of the mammalian cytoskeleton and expressed in cell-type-specific patterns. Morphological changes during cell differentiation are linked to IF network remodeling. However, little is known concerning the presence and the role of IFs in embryonic stem (ES) cells and during their differentiation.ResultsWe have examined the expression profile of synemin isoforms in mouse pluripotent ES cells and during their neural differentiation induced by retinoic acid. Using RT-PCR, Western blotting and immunostaining, we show that synemin M is present at both mRNA and protein levels in undifferentiated ES cells as early as pluripotency factor Oct-3/4 and IF keratin 8. Synemin H was produced only in neural precursors when neural differentiation started, concurrently with synemin M, nestin and glial fibrillary acidic protein. However, both synemin H and M were restricted to the progenitor line during the neural differentiation program. Our in vivo analysis also confirmed the expression of synemins H/M in multipotent neural stem cells in the subventricular zone of the adult brain, a neurogenic germinal niche of the mice. Knocking down synemin in ES cells by shRNA lentiviral particles transduction has no influence on expression of Oct4, Nanog and SOX2, but decreased keratin 8 expression.ConclusionsOur study shows a developmental stage specific regulation of synemin isoforms in ES cells and its neural derivatives. These findings represent the first evidence that synemins could potentially be useful markers for distinguishing multipotent ES cells from undifferentiated neural stem cells and more committed progenitor cells.

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Section: Discussionmentioning

confidence: 99%