Intermediate filament expression and lectin histochemical features of canine transmissible venereal tumour

Abstract

Idiart, J. R. Intermediate filament expression and lectin histochemical features of canine transmissible venereal tumour. APMIS 103: 645-650, 1995. Immunocharacterization of intermediate filament proteins and lectin-binding studies were carried out in canine transmissible venereal tumour (TVT), a unique neoplasia sharing some epidemiological features with Kaposi's sarcoma in humans. Neoplastic cells express vimentin, but neither cytokeratin nor desmin. Regarding lectins, TVT cells express receptors for Triticum vulgaris (WGA), Concanavalia ensiformis (Con A) and Ricinus communis I (RCA-I). They appear to be negative for Ulex europaeus-I (UEA-I), Arachis hypogaea (PNA), Glycine maximus (SBA) and Dolichos biflorus (DBA).

Clonally transmissible cancers in dogs and Tasmanian devils

“…CTVT cells do not have many distinctive ultrastructural features (Murray et al, 1969;HernandezJauregui et al, 1973;Cockrill and Beasley, 1975;Kennedy et al, 1977;Hill et al, 1984). Owing to its expression of lysozyme, a-1-antitrypsin, NSE and vimentin, CTVT has been proposed to be derived from the macrophage lineage (Table 1; Sandusky et al, 1987;Gimeno et al, 1995;Mozos et al, 1996;Marchal et al, 1997;Mukaratirwa et al, 2004). This diagnosis is supported by the observation that CTVT cells themselves may be parasitized by Leishmania infantum, an organism that normally infects macrophages (Albanese et al, 2002;Catone et al, 2003).…”

Glycoconjugates in the branchial mucous cells of <i>Cynoscion guatucupa</i> (Cuvier, 1830) (Pisces: Sciaenidae)

“…The reagent from the Vector-kit is the most sensitive and specific for examining GCs using the biotinylated lectins and the avidin-biotin-peroxidase complex (ABC) (Ellis and Holliday, 1992). Lectin staining methodologies were done according to Gimeno et al (1995). Paraffin sections were deparaffinized with xylene and incubated in 0.3% H 2 O 2 in methanol for 30 min at room temperature in order to block endogenous peroxidase activity.…”

Lectin histochemical aspects of the ovarian lamellae epithelium of Genypterus blacodes (Schneider, 1801)

“…Biotinylated lectins were used to identify speciϐic sugar residues of GCs. Lectin staining methodologies were done according to Gimeno et al (1995). Parafϐin sections mounted on slides coated with Poly-L-lisine (Sigma Diagnostics, St Louis, MO, USA) were deparafϔinised with xylene and then incubated in 0.3% H 2 O 2 in methanol for 30 min at room temperature in order to block the endogenous peroxidase activity.…”