Interleukin (IL)-23 Suppresses IL-10 in Inflammatory Bowel Disease

Liu, Zhanju; Feng, Bai–Sui; Yang, Shaobo; Chen, Xiao; Su, Jie; Yang, Ping‐Chang

doi:10.1074/jbc.m111.304949

Cited by 40 publications

(30 citation statements)

References 38 publications

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“…The positive cells and total stromal cells were counted in the LP. The proportion of positive cells was calculated as follows: [(positive cells)/(total cell number)] ¥ 100 [23][24][25].…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…The sensitivity of each assay was 10 pg/ml. Cultured cells were then collected followed by extraction of total RNA; mRNA levels were analysed for evaluation of TNF, IFN-g, IL-17A, IL-21 and RORC, as described previously [15,[23][24][25].…”

Section: Isolation and Culture Of Cd4mentioning

confidence: 99%

“…All PCR reactions were run in triplicate and performed with 40 cycles using the following conditions: 95°C for 1 min, followed by 40 cycles at 95°C for 15 s and 60°C for 30 s. The relative target gene expression levels were calculated as a ratio relative to the b-actin reference mRNA. Quantitative real-time PCR analysis was carried out using the 2-᭝᭝Ct method [15,23,24].…”

Section: Quantitative Real-time Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

See 2 more Smart Citations

Anti-tumour necrosis factor therapy enhances mucosal healing through down-regulation of interleukin-21 expression and T helper type 17 cell infiltration in Crohn's disease

Liu

Xia

et al. 2013

Clinical and Experimental Immunology

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“…The positive cells and total stromal cells were counted in the LP. The proportion of positive cells was calculated as follows: [(positive cells)/(total cell number)] ¥ 100 [23][24][25].…”

Section: Immunohistochemistrymentioning

confidence: 99%

Section: Isolation and Culture Of Cd4mentioning

confidence: 99%

Section: Quantitative Real-time Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

See 1 more Smart Citation

Anti-tumour necrosis factor therapy enhances mucosal healing through down-regulation of interleukin-21 expression and T helper type 17 cell infiltration in Crohn's disease

Liu

Xia

et al. 2013

Clinical and Experimental Immunology

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“…Cell Targets of Th17 Cells in the Gut  mice, IL-23R is also associated with inhibition of regulatory T cells and the production of IL-10 [185][186][187]. Importantly, Th17-derived cytokines are also produced by other hematopoietic and nonhematopoietic cells including CD8+ T cells [188,189], γ δ T cells [190], innate lymphoid cells [164,191], iNKT cells [192], mast cells [193,194], neutrophils [195,196], Paneth cells [109], and B cells [167].…”

Section: Resultsmentioning

confidence: 94%

Molecular Mechanisms and Cell Targets of Th17 Cells in the Gastrointestinal Tract: An Innate Sense of Adaptivity

Verdier

Ruemmele

2013

International Reviews of Immunology

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T-helper (TH) 17 activation is crucial for protective immune responses against bacteria and fungi at mucosal surfaces, but it can also be implicated in the pathogenesis of several autoimmune and chronic inflammatory diseases, such as inflammatory bowel diseases (IBD). Although rapid progress was made elucidating induction and functional heterogeneity of Th17 responses, the underlying molecular effects of Th17 response including the most relevant different cell targets of Th17 cytokines remain poorly understood. Cytokines produced by Th17 cells have broad effects on both hematopoietic and nonhematopoietic cells and can act in synergy with various inflammatory factors. In this review, we will focus on the effects of Th17-derived cytokines in the gastrointestinal tract and discuss how Th17 responses can affect both innate and adaptive immunity and may contribute to the pathogenesis of inflammatory GI processes.

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“…The imbalance between pro-inflammatory and anti-inflammatory cytokines participates in the pathogenesis and progression of IBD. For example, overexpression of IL-23 in the inflamed mucosa of IBD patients has been found to promote Th17 cell differentiation and inhibit IL-10 production [4] . However, IL-25 is observed to be markedly decreased in the inflamed mucosa of IBD patients and can promote IL-10 production and suppress TNF-α, IFN-γ and IL-17A secretion [5] .…”

Section: Introductionmentioning

confidence: 99%

ASCA, ANCA, ALCA and Many More: Are They Useful in the Diagnosis of Inflammatory Bowel Disease?

Zhou

Song

Yang

et al. 2016

Dig Dis

Self Cite

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Background: Inflammatory bowel disease (IBD) is characterized by excessive immune responses to altered intestinal microbiota in genetically susceptible individuals. The diagnosis of IBD depends on clinical, endoscopic, histological, radiological and biochemical criteria, which may be invasive, time consuming and usually not accepted by patients with IBD. Key Messages: Serological biomarkers have been demonstrated to be a series of rapid, non-invasive approaches for assessments of early diagnosis, disease activity and prognosis for IBD. Importantly, serum antibodies against microbial antigens or auto-antigens have been used as biomarkers in predicting disease course, complications and responses to medications and surgery. Moreover, they have been demonstrated to be useful in distinguishing patients with Crohn's disease (CD) from those with ulcerative colitis (UC). Recently, a great number of new serum biomarkers (e.g., anti-glycoprotein 2, anti-granulocyte macrophage colony-stimulating factor, anti-neutrophil cytoplasmic antibody, anti-Saccharomyces cerevisiae antibody, anti-laminaribioside carbohydrate IgG antibody, anti-mannobioside carbohydrate IgG antibody, antibody to the outer membrane protein of Escherichia coli, anti-CBir1) have been found to be present in patients with IBD and are potentially used in the diagnosis and prediction. The presence of these antibodies in the sera is due to the disruption of intestinal mucosa barrier and they may reflect a possibly genetic loss of immunological tolerance toward microbiota-derived antigens. Due to their non-invasive, easily accessible, repetitive and economical characteristics, these biomarkers have been found to serve as precious supplementary means in the diagnosis and disease evaluation of IBD. Conclusions: Currently, the most important utility of serological biomarkers is to evaluate the aggressive risks of disease phenotype, complications or surgery requirement, predict prognosis of the disease and distinguish CD from UC. However, they have limited values in making initially definite diagnosis for IBD. Therefore, more effective biomarkers with high sensitivity and specificity need to be further explored in the future.

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Interleukin (IL)-23 Suppresses IL-10 in Inflammatory Bowel Disease

Cited by 40 publications

References 38 publications

Anti-tumour necrosis factor therapy enhances mucosal healing through down-regulation of interleukin-21 expression and T helper type 17 cell infiltration in Crohn's disease

Anti-tumour necrosis factor therapy enhances mucosal healing through down-regulation of interleukin-21 expression and T helper type 17 cell infiltration in Crohn's disease

Molecular Mechanisms and Cell Targets of Th17 Cells in the Gastrointestinal Tract: An Innate Sense of Adaptivity

ASCA, ANCA, ALCA and Many More: Are They Useful in the Diagnosis of Inflammatory Bowel Disease?

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