INTERLEUKIN-8, TUMOR NECROSIS FACTOR-α, AND LACTOFERRIN IN IMMUNOCOMPETENT HOSTS WITH EXPERIMENTAL AND BRAZILIAN CHILDREN WITH ACQUIRED CRYPTOSPORIDIOSIS

ALCANTARA, CIRLE S.; White, A. Clinton; Okhuysen, Pablo C.; Lima, Aldo Ângelo Moreira; Guerrant, Richard L.; YANG, CHANG-HUN; BARRETT, LEAH J.; STEINER, THEODORE S.; Chappell, Cynthia L.

doi:10.4269/ajtmh.2003.68.325

Cited by 58 publications

(50 citation statements)

References 18 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Internalization rates were estimated using early time data (0-30 minutes) to avoid confounding effects of recycling (Fig. 2D) and ranged from 0.008 to 0.045 1/min, consistent with published HER2 internalization rates (33,37,38).…”

Section: Internalizationsupporting

confidence: 54%

“…Because HER2-tPLD internalization rates (see Fig. 3) were similar to that of HER2 (33,37,38), it was assumed that binding to and cross-linking of HER2 had no effect on internalization rate. Once inside the cell, doxorubicin is released from HER2-tPLD with first-order kinetics, characterized by rate k rel_cell and stoichiometry of 20,000 doxorubicin molecules per liposome.…”

Section: Kinetic Computational Modelmentioning

confidence: 99%

See 1 more Smart Citation

Impact of Tumor HER2/ERBB2 Expression Level on HER2-Targeted Liposomal Doxorubicin-Mediated Drug Delivery: Multiple Low-Affinity Interactions Lead to a Threshold Effect

Hendriks

Klinz

Reynolds

et al. 2013

Molecular Cancer Therapeutics

View full text Add to dashboard Cite

Numerous targeted nanotherapeutics have been described for potential treatment of solid tumors. Although attention has focused on antigen selection and molecular design of these systems, there has been comparatively little study of how cellular heterogeneity influences interaction of targeted nanoparticles with tumor cells. Antigens, such as HER2/ERBB2, are heterogeneously expressed across different indications, across patients, and within individual tumors. Furthermore, antigen expression in nontarget tissues necessitates optimization of the therapeutic window. Understanding the performance of a given nanoparticle under different regimens of antigen expression has the ability to inform patient selection and clinical development decisions. In this work, HER2-targeted liposomal doxorubicin was used as a modeltargeted nanoparticle to quantitatively investigate the effect of HER2 expression levels on delivery of doxorubicin to the nucleus. We find quantitatively greater nuclear doxorubicin delivery with increasing HER2 expression, exhibiting a threshold effect at approximately 2 Â 10 5 HER2 receptors/cell. Kinetic modeling indicated that the threshold effect arises from multiple low-affinity interactions between the targeted liposome and HER2. These results support previous data showing little or no uptake into human cardiomyocytes, which express levels of HER2 below the threshold. Finally, these results suggest that HER2-targeted liposomal doxorubicin may effectively target tumors that fall below traditional definitions of HER2-positive tumors, thereby expanding the potential population of patients that might benefit from this agent. Mol Cancer Ther; 12(9); 1816-28. Ó2013 AACR.

show abstract

Section: Internalizationsupporting

confidence: 54%

Section: Kinetic Computational Modelmentioning

confidence: 99%

Impact of Tumor HER2/ERBB2 Expression Level on HER2-Targeted Liposomal Doxorubicin-Mediated Drug Delivery: Multiple Low-Affinity Interactions Lead to a Threshold Effect

Hendriks

Klinz

Reynolds

et al. 2013

Molecular Cancer Therapeutics

View full text Add to dashboard Cite

show abstract

“…Abdel-Messih et al (2005) in Egypt demonstrated that clinical findings associated with Cryptosporidium diarrhea included vomiting, persistent diarrhea and the need for hospitalization. Studies by Alcantara et al (2003) indicated that Cryptosporidium was associated with inflammation as indicated by the lactoferrin test and the presence of IL8 and TNF-. In this study, Cryptosporidium was also associated with inflammation and more than 59.1% of Cryptosporidium infections might lead to inflammation.…”

Section: Discussionsupporting

confidence: 57%

Bacterial and Parasitic Agents of Infectious Diarrhoea in the Era of HIV and AIDS - The Case of a Semi Rural Community in South Africa

Samie¹,

Bessong²,

L³

et al. 2011

Microbes, Viruses and Parasites in AIDS Process

View full text Add to dashboard Cite

“…This is reminiscent of Cryptosporidium in developing countries, which has been shown to stimulate an inflammatory response in malnourished children (Alcantara et al, 2003;Kirkpatrick et al, 2002). It was also independent of co-infections with EAEC infections, which have also been associated with increased faecal lactoferrin in this population (Steiner et al, 1998).…”

Section: Discussionmentioning

confidence: 79%

Giardia duodenalis assemblage, clinical presentation and markers of intestinal inflammation in Brazilian children

Kohli

Bushen

Pinkerton

et al. 2008

Transactions of the Royal Society of Tropical Medicine and Hygi

Self Cite

136

View full text Add to dashboard Cite

SummaryData on the relationship between the two genotypes of Giardia duodenalis that infect humans, assemblages A and B, their clinical presentation and intestinal inflammation are limited. We analyzed 108 stool samples previously collected for a diarrhoeal study among Brazilian children, representing 71 infections in 47 children. Assemblage B was most prevalent, accounting for 43/58 (74.1%) infections, while assemblage A accounted for 9/58 (15.5%) infections and 6/58 (10.3%) infections were mixed (contained both assemblage A and B). There was no significant difference in diarrhoeal symptoms experienced during assemblage A, B or mixed infections. Children with assemblage B demonstrated greater variability in G. duodenalis cyst shedding but at an overall greater level (n = 43, mean 3.6 × 10 5 , range 5.3 × 10 2 -2.5 × 10 6 cysts/ml) than children infected with assemblage A (n = 9, mean 1.4 × 10 5 , range 1.5 × 10 4 -4.6 × 10 5 cysts/ml; P = 0.009). Children with mixed infections shed more cysts (mean 8.3 × 10 5 , range 3.1 × 10 4 -2.8 × 10 6 cysts/ml) than children with assemblage A or B alone (P = 0.069 and P = 0.046 respectively). This higher rate of cyst shedding in children with assemblage B may propagate its spread, accounting for its increased incidence. Additionally, second and third infections had decreasing faecal lactoferrin, suggesting some protection against severity, albeit not against infection, by prior infection. Authors' contributions: AAML, RLG, AK and OYB designed the study protocol; RDN, AK and OYB carried out the clinical assessment; AK carried out and EH designed and carried out the quantitative PCR of Giardia; AK, RCP, OYB, CLS and RDN carried out the analysis and interpretation of these data; AK, OYB, RDN and CLS drafted the manuscript. All authors read and approved the final manuscript. AK and RLG are guarantors of the paper.Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Conflicts of interest: None declared.Ethical approval: The study protocol and informed consent were approved by the human investigations committees of the Federal

show abstract

INTERLEUKIN-8, TUMOR NECROSIS FACTOR-α, AND LACTOFERRIN IN IMMUNOCOMPETENT HOSTS WITH EXPERIMENTAL AND BRAZILIAN CHILDREN WITH ACQUIRED CRYPTOSPORIDIOSIS

Cited by 58 publications

References 18 publications

Impact of Tumor HER2/ERBB2 Expression Level on HER2-Targeted Liposomal Doxorubicin-Mediated Drug Delivery: Multiple Low-Affinity Interactions Lead to a Threshold Effect

Impact of Tumor HER2/ERBB2 Expression Level on HER2-Targeted Liposomal Doxorubicin-Mediated Drug Delivery: Multiple Low-Affinity Interactions Lead to a Threshold Effect

Bacterial and Parasitic Agents of Infectious Diarrhoea in the Era of HIV and AIDS - The Case of a Semi Rural Community in South Africa

Giardia duodenalis assemblage, clinical presentation and markers of intestinal inflammation in Brazilian children

Contact Info

Product

Resources

About