Interleukin-8 Transcripts in Mononuclear Cells Determine Impaired Graft Function after Kidney Transplantation

Borst, Christoffer; Xia, Shujie; Bistrup, Claus; Tepel, Martín

doi:10.1371/journal.pone.0117315

Cited by 14 publications

(28 citation statements)

References 27 publications

(23 reference statements)

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“…Borst et al. have shown that reduced levels of IL-8 transcripts in peripheral mononuclear cells predict immediate graft dysfunction and delayed graft function [ 38 ]. In the study by Singh, IL-8 –251AA genotype was associated with 2.7-fold increased risk for allograft rejection in recipients experiencing rejection episodes [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Urinary IL-8 is a marker of early and long-term graft function after renal transplantation

et al. 2017

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In this study, we examined whether the IL-8 content of urine sampled on day 1 and day 14 after renal transplantation is a marker of early and long-term renal function. Moreover, we assessed whether its concentration is positively correlated with the matrix metalloproteinase-9 (MMP-9) content of urine sampled on day 1 and day 30 and 12 months after renal transplantation. Our analysis covered 87 patients who underwent a kidney transplant. The patients were observed for an average of 30 months (12–60 months). The IL-8 concentration determined on day 1 was significantly negatively correlated with creatinine clearance early after renal transplantation (on days 1, 7, 14 and 30), as well as during long-term observations. IL-8 concentration in urine sampled on day 1 and day 14 was higher in patients demonstrating DGF than in those without DGF. No relationship was found between IL-8 content and cold ischaemia time. MMP-9 activity determined on day 1 and month 3 after renal transplantation was positively correlated with the IL-8 content determined in urine sampled on day 1, Rs = +0.32, p < .05 and Rs = +0.31, p < .05, respectively. The results of this study suggest that a high IL-8 content in urine sampled on day 1 after renal transplantation is an unfavourable marker of early and long-term (years-long) graft function. A high IL-8 content in urine sampled on day 1 after renal transplantation was positively correlated with the activity of metalloproteinase-9 in urine. This proves that both of these chemokines cooperate in ischaemia–reperfusion injuries in transplanted kidneys.

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Section: Discussionmentioning

confidence: 99%

Urinary IL-8 is a marker of early and long-term graft function after renal transplantation

et al. 2017

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“…Transcripts of MGAT3-AS1, C5aR1, and β-actin were measured with quantitative real-time reverse transcriptase polymerase chain reactions (qRT-PCR). The primers were as follows as previously established 6,16 .…”

Section: Methodsmentioning

confidence: 99%

“…Delayed graft function was defined by at least one dialysis session within 7 days of transplantation 16,18 .…”

Section: Methodsmentioning

confidence: 99%

Endogenous intronic antisense long non-coding RNA, MGAT3-AS1, and kidney transplantation

Nagarajah

Xia

Rasmussen

et al. 2019

Sci Rep

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β-1,4-mannosylglycoprotein 4-β-N-acetylglucosaminyltransferase (MGAT3) is a key molecule for the innate immune system. We tested the hypothesis that intronic antisense long non-coding RNA, MGAT3-AS1, can predict delayed allograft function after kidney transplantation. We prospectively assessed kidney function and MGAT3-AS1 in 129 incident deceased donor kidney transplant recipients before and after transplantation. MGAT3-AS1 levels were measured in mononuclear cells using qRT-PCR. Delayed graft function was defined by at least one dialysis session within 7 days of transplantation. Delayed graft function occurred in 22 out of 129 transplant recipients (17%). Median MGAT3-AS1 after transplantation was significantly lower in patients with delayed graft function compared to patients with immediate graft function (6.5 × 10−6, IQR 3.0 × 10−6 to 8.4 × 10−6; vs. 8.3 × 10−6, IQR 5.0 × 10−6 to 12.8 × 10−6; p < 0.05). The median preoperative MGAT3-AS1 was significantly lower in kidney recipients with delayed graft function (5.1 × 10−6, IQR, 2.4 × 10−6 to 6.8 × 10−6) compared to recipients with immediate graft function (8.9 × 10−6, IQR, 6.8 × 10−6 to 13.4 × 10−6; p < 0.05). Receiver-operator characteristics showed that preoperative MGAT3-AS1 predicted delayed graft function (area under curve, 0.83; 95% CI, 0.65 to 1.00; p < 0.01). We observed a positive predictive value of 0.57, and a negative predictive value of 0.95. Long non-coding RNA, MGAT3-AS1, indicates short-term outcome in patients with deceased donor kidney transplantation.

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“…Alterações na expressão gênica demonstraram serem capazes de predizer perdas na função renal (Neuwirt et al, 2014;Borst et al, 2015). Neuwirt e colaboradores identificaram e validaram um painel de três genes (BMP7, THBS1 e TRIB1), cujas expressões em biópsias renais foram capazes de predizer concentrações de creatinina sérica em um período de até 28 meses (Neuwirt et al, 2014).…”

Section: Tabela 4 -Dados Da Eficiência Dos Ensaios De Expressão Mrna unclassified

“…Neuwirt e colaboradores identificaram e validaram um painel de três genes (BMP7, THBS1 e TRIB1), cujas expressões em biópsias renais foram capazes de predizer concentrações de creatinina sérica em um período de até 28 meses (Neuwirt et al, 2014). Já Borst e colaboradores, verificaram que redução na expressão de mRNA do gene da interleucina-18, em células mononucleares do sangue periférico, são capazes de predizer aumento na creatinina sérica e presença de função do enxerto retardada (do inglês delayed graft function), uma forma de falência renal aguda, em até 24h póstransplante (Borst et al, 2015).…”

Section: Tabela 4 -Dados Da Eficiência Dos Ensaios De Expressão Mrna unclassified

Relação entre o perfil de expressão de genes envolvidos na farmacodinâmica de imunossupressores e de microRNAs reguladores, com a resposta terapêutica em transplantados renais

Bonezi¹

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por ter inicialmente aberto as portas do laboratório e permitido o meu ingresso no Programa de Aprimoramento, bem como por toda a contribuição no meu crescimento intelectual. À minha orientadora Profa. Dra. Rosario D. C. Hirata, por ter acreditado no meu potencial e confiado em mim para a realização deste trabalho. Pela imensurável paciência e dedicação. E por todas as horas desprendidas comigo me orientando e participando ativamente do meu crescimento profissional, pessoal e intelectual. Muito obrigada. Aos meus colegas de laboratório, que além de me auxiliarem em todo o trabalho, tornaram os meus dias muito mais prazerosos. Sem a companhia e ajuda de vocês eu certamente não teria conseguido. À Cristina M. Fajardo, nossa técnica, por todo auxílio e companhia. Aos meus familiares, em especial ao meu irmão, que me aturou com imensa compreensão e apoio, nos altos e baixos, sempre me motivando, e aos meus pais pelo suporte dado durante essa jornada que é a pós-graduação. Amo vocês.

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Interleukin-8 Transcripts in Mononuclear Cells Determine Impaired Graft Function after Kidney Transplantation

Cited by 14 publications

References 27 publications

Urinary IL-8 is a marker of early and long-term graft function after renal transplantation

Urinary IL-8 is a marker of early and long-term graft function after renal transplantation

Endogenous intronic antisense long non-coding RNA, MGAT3-AS1, and kidney transplantation

Relação entre o perfil de expressão de genes envolvidos na farmacodinâmica de imunossupressores e de microRNAs reguladores, com a resposta terapêutica em transplantados renais

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