Interleukin-8 Release Inhibitors Generated by Fermentation of Artemisia princeps Pampanini Herb Extract With Lactobacillus plantarum SN13T

Okamoto, Tomoko; Sugimoto, Sachiko; Noda, Masafumi; Yokooji, Tomoharu; Danshiitsoodol, Narandalai; Higashikawa, Fumiko; Sugiyama, Masanori

doi:10.3389/fmicb.2020.01159

Cited by 15 publications

(12 citation statements)

References 48 publications

(66 reference statements)

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“…Our study shows that both IL-8 and IL-1β are reduced in infected D6234 primary oral epithelial cells in the presence of Auraguard, suggesting that the plaque-derived bacterial pathogens will have a lesser impact on the host oral epithelium. Plant extracts are accountable for similar results on IL-8 reduction, and this pattern of reduced IL-8 secretion was demonstrated with plant extracts on HuH-7 cells [49] and Caco-2 cells [33]. It is also possible that the reduced IL-8 secretion is caused by the reduced IL-1β secretion, as it has been reported that IL-1β mediates IL-8 secretion in some instances [33].…”

Section: Discussionsupporting

confidence: 72%

Natural Antimicrobials Promote the Anti-Oxidative Inhibition of COX-2 Mediated Inflammatory Response in Primary Oral Cells Infected with Staphylococcus aureus, Streptococcus pyogenes and Enterococcus faecalis

Butucel

Balta²,

Bunduruș³

et al. 2023

Antioxidants

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Staphylococcus aureus, Streptococcus pyogenes and Enterococcus faecalis can colonize the tooth root canals, adhere to dentin walls, and frequently cause periodontitis in dogs. Bacterial periodontal diseases are common in domesticated pets, causing severe oral cavity inflammation and a strong immune response. This study investigates the antioxidant effect of a natural antimicrobial mixture (Auraguard—Ag) on the ability of S. aureus, S. pyogenes and E. faecalis to infect primary canine oral epithelial cells as well as its impact on their virulence factors. Our data show that a concentration of 0.25% Ag is sufficient to inhibit the growth of all three pathogens, whereas a concentration of 0.5% will become bactericidal. The sub-inhibitory concentration of 0.125% Ag reveals that the antimicrobial mixture can significantly reduce biofilm formation and exopolysaccharide production. The impact on these virulence factors was further translated into a significantly reduced ability to infect primary canine oral epithelial cells and restore epithelial tight junctions, with no impact on the epithelial cell viability. The post-infection inflammatory cytokines (IL-1β and IL-8) and the COX-2 mediator were also reduced both in mRNA and protein expression levels. The oxidative burst, detected upon infection, was also decreased in the presence of Ag, as our results show a significant decrease in H2O2 released by the infected cells. We show that inhibition of either NADPH or ERK activity will result in a downregulation of COX-2 expression and lower levels of H2O2 in infected cells. Conclusively, our study shows that natural antimicrobials reduce pro-inflammatory events, post infection, through an antioxidative mechanism that involves the downregulation of the COX-2 mediator via the inactivation of ERK in the absence of H2O2. As a result, they significantly reduce the risk of secondary bacterial infections and host oxidative stress caused by Staphylococcus aureus, Streptococcus pyogenes and Enterococcus faecalis accumulation in biofilms in an in vitro canine oral infection model.

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Section: Discussionsupporting

confidence: 72%

Natural Antimicrobials Promote the Anti-Oxidative Inhibition of COX-2 Mediated Inflammatory Response in Primary Oral Cells Infected with Staphylococcus aureus, Streptococcus pyogenes and Enterococcus faecalis

Butucel

Balta²,

Bunduruș³

et al. 2023

Antioxidants

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“…Previously, extracts from medicinal plants like Artemisia princeps Pampanini and Paeonia lactiflora Pall had been fermented with plant-derived L. plantarum SN13T and L. brevis 174A, respectively, which generated additional bioactive compounds in the plant extracts (Okamoto et al, 2020;Shakya et al, 2021). In this study, we further attempted to ferment P. lactiflora Pall (Paeonia Radix Alba) and Carthamus tinctorius extracts with PLA-producing strains MSC-C2 and K40.…”

Section: Discussionmentioning

confidence: 99%

“…Our research group has isolated over 1,200 strains of plant-derived LAB from fruits, vegetables, flowers, and medicinal plants and evaluated their health benefits, such as immune modulation, liver function improvement, and reduction of obesity (Jin et al, 2010;Zhao et al, 2012;Noda et al, 2018). Further, when cultivated in plant extracts, some of the plant-derived LAB-produced substances that have shown therapeutic potential (Okamoto et al, 2020;Shakya et al, 2021). Therefore, we also determined the PLA production by L. plantarum MSC-C2 isolated from sugarcane and P. pentosaceus K40 isolated from banana in extracts from the medicinal plants Paeonia lactiflora Pall and Carthamus tinctorius and studied the potential of PLA-containing LAB-medicinal plant extracts against the biofilm and virulence genes of periodontopathogen A. actinomycetemcomitans.…”

Section: Introductionmentioning

confidence: 99%

3-Phenyllactic acid generated in medicinal plant extracts fermented with plant-derived lactic acid bacteria inhibits the biofilm synthesis of Aggregatibacter actinomycetemcomitans

Shakya

Danshiitsoodol²,

Noda³

et al. 2022

Front. Microbiol.

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In the present study, the effect of PLA on a periodontic pathogen, Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), the biofilm, and virulence-related genes was investigated. We confirmed that two lactic acid bacteria (LAB) strains isolated from plant sources, Lactiplantibacillus plantarum MSC-C2 and Pediococcus pentosaceus K40, secrete PLA into the de Man, Rogosa & Sharpe (MRS) broth when supplemented with phenyl pyruvic acid (PPA) as a precursor to PLA. Moreover, PLA was generated in the fermentation broths of two medicinal plant extracts, Paeonia lactiflora Pall (PR) and Carthamus tinctorius (CT), when used by each LAB strain and each extract supplemented with PPA. We determined that the minimum inhibitory concentration (MIC) of PLA against A. actinomycetemcomitans was 20 mM. PLA significantly decreased biofilm formation and suppressed the transcription of pgA, ltxA, and cdtB genes, which encode the poly-N-acetylglucosamine (PGA) polysaccharide of biofilm matrix and exotoxins leukotoxin and cytolethal distending toxin (CDT), respectively. The PLA produced by the MSC-C2 and K40 strains was increased several times by the addition of PPA to the MRS broth. The anti-biofilm effect of the extracts from the fermentation broth was proportional to the increasing PLA concentration, while a cumulatively higher effect than that of PLA alone suggested a combinational effect of PLA and the other metabolites, such as lactic acid (LA). Among the two medicinal plants, PLA, produced after the addition of PPA, was higher in PR extract in case of both the LAB strains. PLA production by the MSC-C2 strain in the PR extract reached 4.8 ± 0.23 mM, which was obviously higher than that in the MRS broth (3.88 ± 0.12 mM) supplemented with 1 mg/ml PPA. The activity to inhibit biofilm formation in the fermented PR extract was clearly high. PLA formed in the fermented PR extract downregulated the dispersin B encoding the dspB gene together with pgA, ltxA, and cdtB. In conclusion, this study shows a promising activity of PLA against the A. actinomycetemcomitans biofilm and virulence genes. In addition, the combinational effect of PLA and the medicinal plant extract can be achieved by fermentation with a specific plant-derived LAB strain.

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“…Furthermore, LAB-fermented plants have been applied to treat diabetes and liver diseases in rats [21], reduce stress [22], and inhibit the proliferation of cancer cell lines better than non-fermented plant extracts [23]. Artemisia princeps Pamp., fermented by Lactobacillus plantarum SN13T, showed therapeutic potential by reducing the release of interleukin-8 in a human hepatoma cell line [24]. In these applications, predominantly single LAB strains were applied.…”

Section: Introductionmentioning

confidence: 99%

Succession of Bacterial and Fungal Communities during Fermentation of Medicinal Plants

et al. 2022

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The fermentation of medicinal plants has been studied very little, as compared to the fermentation of food and beverages. One approach applies fermentation by single bacterial or fungal strains and targets the production of specific compounds or preservation of the fermented material. Spontaneous fermentation by an autochthonous starter community may lead to a more diverse blend of fermentation products because co-occurring microbes may activate the biosynthetic potentials and formation of compounds not produced in single strain approaches. We applied the community approach and studied the fermentation of four medicinal plants (Achillea millefolium, Taraxacum officinale, Mercurialis perennis, and Euphrasia officinalis), according to a standardized pharmaceutical fermentation method. It is based on the spontaneous fermentation by plant-specific bacterial and fungal communities under a distinct temperature regime, with a recurrent cooling during the first week and further fermentation for at least six months. The results revealed both general and plant-specific patterns in the composition and succession of microbial communities during fermentation. Lactic acid bacteria increasingly dominated in all preparations, whereas the fungal communities retained more plant-specific features. Three distinct fermentation phases with characteristic bacterial communities were identified, i.e., early, middle, and late phases. Co-occurrence network analyses revealed the plant-specific features of the microbial communities.

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Interleukin-8 Release Inhibitors Generated by Fermentation of Artemisia princeps Pampanini Herb Extract With Lactobacillus plantarum SN13T

Cited by 15 publications

References 48 publications

Natural Antimicrobials Promote the Anti-Oxidative Inhibition of COX-2 Mediated Inflammatory Response in Primary Oral Cells Infected with Staphylococcus aureus, Streptococcus pyogenes and Enterococcus faecalis

Natural Antimicrobials Promote the Anti-Oxidative Inhibition of COX-2 Mediated Inflammatory Response in Primary Oral Cells Infected with Staphylococcus aureus, Streptococcus pyogenes and Enterococcus faecalis

3-Phenyllactic acid generated in medicinal plant extracts fermented with plant-derived lactic acid bacteria inhibits the biofilm synthesis of Aggregatibacter actinomycetemcomitans

Succession of Bacterial and Fungal Communities during Fermentation of Medicinal Plants

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