Interleukin-8 potentiates the effect of interleukin-1-induced uterine contractions

Khatun, Selina; Kanayama, Naohiro; Belayet, Hossain Md; Yonezawa, Masumi; Kobayashi, Takao; Terao, Toshihiko

doi:10.1093/humrep/14.2.560

Cited by 23 publications

(16 citation statements)

References 22 publications

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“…The mechanism involved in the up-regulation of PGHS-2 activity with the onset of labour is uncertain, but several stimuli including IL-1b are known to increase PGHS-2 expression via the transcription factor nuclear factor k B (NFkB) (Allport et al 2001). Indeed, most data suggest that inflammatory cytokines increase myometrial contractility via increased PG synthesis (Khatun et al 1999). The multiple regression data revealed associations between the levels of PGHS-2 mRNA and those of IL-8, IL-6 and IL-1b and our in vitro studies confirmed the important role played by IL-1b; in contrast both IL-6 and IL-8 had a small effect.…”

Section: Discussionmentioning

confidence: 99%

Pro-labour myometrial gene expression: are preterm labour and term labour the same?

Tattersall¹,

Engineer²,

Khanjani³

et al. 2008

Reproduction

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Preterm labour (PTL) is the most important cause of neonatal morbidity and mortality. While some causes have been identified, the mechanisms involved remain elusive. This study investigates whether term labour (TL) is an appropriate model for PTL by examining prolabour gene expression, using quantitative rtPCR, and protein synthesis, using Western analysis, in preterm and term myometrial samples obtained from the upper and lower uterine segments before and after the onset of labour. In the lower segment, the levels of prostaglandin H synthase type-2 (PGHS-2), interleukin-1b (IL-1b), IL-6 and IL-8 mRNA expression were significantly higher in TL compared with PTL samples. Compared with non-labour controls, the expression of IL-1b and IL-8 mRNA was increased in both PTL and TL samples and the expression of PGHS-2 and IL-6 mRNA was increased in TL samples only. In the upper segment, there were no differences between PTL and TL samples and the mRNA expression of PGHS-2 and IL-1b was increased in TL compared with term no labour samples. No effect of PTL or TL was seen on either oxytocin receptor or connexin-43 mRNA expression or protein levels. The multiple regression analysis and studies in primary cultures of uterine myocytes suggest that the inflammatory cytokines, IL-1b and tumour necrosis factor-a, are the most important regulators of PGHS-2 and IL-8. Our data show that preterm and term labouring myometrium are significantly different and that the most marked labour-induced changes in gene expression are in the lower segment. These changes may occur in response to the release of inflammatory cytokines by the labour-associated inflammatory infiltration. Reproduction (2008) 135 569-579

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Section: Discussionmentioning

confidence: 99%

Pro-labour myometrial gene expression: are preterm labour and term labour the same?

Tattersall¹,

Engineer²,

Khanjani³

et al. 2008

Reproduction

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“…For example, IL-1b enhances contraction of airway, uterine and gastrointestinal smooth muscles. (19)(20)(21) Andre et al (22) reported IFN caused contraction of primary cultures of rat vascular smooth muscle cells that was blocked by verapamil. Cytokine potentiation of bronchoconstrictors such as acetylcholine, histamine and leukotrienes is also probably an important component of airway hyper-reactivity seen in asthma.…”

Section: Production Of Cytokines and Chemokines By Smooth Musclesmentioning

confidence: 99%

Synthesis of immune modulators by smooth muscles

et al. 2004

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The primary function of smooth muscle cells is to contract and alter the stiffness or diameter of hollow organs such as blood vessels, the airways and the gastrointestinal and urogenital tracts. In addition to purely structural functions, smooth muscle cells may play important metabolic roles, particularly in various inflammatory responses. In cell culture, these cells have been shown to be metabolically dynamic, synthesizing and secreting extracellular matrix proteins, glycosaminoglycans and a wide variety of cell-cell signaling proteins, such as interleukins, chemokines and peptide growth factors. Secreted cell signaling proteins participate in the inflammatory response of smooth muscle-containing organs, and some can also stimulate smooth muscle migration, proliferation and contraction. The cellular signaling pathways controlling synthesis of these signaling proteins are similar to those used by cells mediating innate immunity and may contribute to pathogenesis of diverse diseases including atherosclerosis, asthma, inflammatory bowel diseases and preterm labor. Appreciating the role of smooth muscle cells in these diseases may lead to better understanding of the beneficial effects of anti-inflammatory drugs as well as identification of new targets for anti-inflammatory therapy.

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“…The degrees of inflammatory infiltrate and cytokine expression correlate (4), and it has been suggested that the invading leukocytes are the major source of the proinflammatory cytokines (6). The proinflammatory cytokines in turn increase leukocyte infiltration and promote both the expression of contraction-associated peptides and uterine contractions (7,8). Furthermore, and more importantly in some circumstances, the proinflammatory cytokines may contribute to fetal morbidity and mortality (9).…”

mentioning

confidence: 99%

“…Its production is increased in myometrium, cervix, decidua, and fetal membranes with labor (4, 10), and its levels are increased in amniotic fluid in the presence of chorioamnionitis and with the duration of labor (11,12). It acts synergistically with IL-8 to increase prostaglandin production and uterine contractions in the rabbit (7), and in vitro in human myometrial cells, it increases prostaglandin H synthase 2 (PGHS-2) expression via p38 MAPK activation and increased nuclear factor B (NFB) DNA binding in myometrium and amnion (13)(14)(15). IL-1␤ also increases IL-8 expression in fetal membranes and lower segment fibroblasts (8) and reduces prostaglandin dehydrogenase activity in the intact fetal membrane disc model (16).…”

mentioning

confidence: 99%

The Mitogen-Activated Protein Kinase Dependent Expression of Prostaglandin H Synthase-2 and Interleukin-8 Messenger Ribonucleic Acid by Myometrial Cells: The Differential Effect of Stretch and Interleukin-1β

Sooranna

Loudon

Terzidou

et al. 2005

The Journal of Clinical Endocrinology &Amp; Metabolism

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Infection and uterine stretch are the common causes of preterm labor. IL-1beta plays a key role in infection-induced preterm labor and increases prostaglandin H synthase 2 (PGHS-2) and IL-8 expression. We have shown that mechanical stretch of uterine myocytes in vitro up-regulates the expression of PGHS-2 and IL-8. In this study, we tested the hypotheses that both IL-1beta and mechanical stretch increase the myometrial expression of PGHS-2 and IL-8 via MAPK activation and that their effects are synergistic. MAPK activation was assessed in myocytes obtained from pregnant women undergoing cesarean section before the onset of labor after exposure to IL-1beta and stretch either alone or in combination. Specific inhibitors of ERK, p38, and c-Jun N-terminal kinase were used to define the role of each in the increased expression of PGHS-2 and IL-8 mRNA. We found that both IL-1beta and stretch activated all three MAPK subtypes but that they had no synergistic effect. The inhibitor studies showed that stretch-induced increases in both PGHS-2 and IL-8 mRNA expression were ERK1/2 and p38 dependent and that IL-1beta-induced increases of PGHS-2 mRNA expression were also ERK1/2 and p38 dependent, but those of IL-8 were dependent only on ERK1/2 activation. These data show that exposure of human uterine myocytes to both stretch and IL-1beta activates the MAPK system, which is responsible for the increase in PGHS-2 and IL-8 mRNA expression. We found no evidence of a synergistic effect of IL-1beta and stretch on myometrial expression of PGHS-2 and IL-8 mRNA.

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Interleukin-8 potentiates the effect of interleukin-1-induced uterine contractions

Cited by 23 publications

References 22 publications

Pro-labour myometrial gene expression: are preterm labour and term labour the same?

Pro-labour myometrial gene expression: are preterm labour and term labour the same?

Synthesis of immune modulators by smooth muscles

The Mitogen-Activated Protein Kinase Dependent Expression of Prostaglandin H Synthase-2 and Interleukin-8 Messenger Ribonucleic Acid by Myometrial Cells: The Differential Effect of Stretch and Interleukin-1β

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