Interleukin-8 is associated with circulating CD133+ progenitor cells in acute myocardial infarction

Schömig, Kathrin; Busch, Gabriele; Steppich, Birgit; Sepp, Dominik; Kaufmann, Jan; Stein, Andreas; Schömig, Albert; Ott, Ilka

doi:10.1093/eurheartj/ehi761

Cited by 108 publications

(97 citation statements)

References 32 publications

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“…IL-8 is another constituent of WPB, which is released upon their exocytosis (21). Secretion of IL-8 after myocardial infarction was found to be associated with mobilization of EPC (23). Hence, it is possible that the reduced level of EPC and HSC mobilization and recruitment to the ischemic kidneys in ITF 1697-treated animals is a manifestation of curtailed release of IL-8 and Ang-2.…”

Section: Discussionmentioning

confidence: 99%

Functional consequences of inhibiting exocytosis of Weibel-Palade bodies in acute renal ischemia

Yasuda

Vasko

Hayek

et al. 2012

American Journal of Physiology-Renal Physiology

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Yasuda K, Vasko R, Hayek P, Ratliff B, Bicer H, Mares J, Maruyama S, Bertuglia S, Mascagni P, Goligorsky MS. Functional consequences of inhibiting exocytosis of Weibel-Palade bodies in acute renal ischemia. Am J Physiol Renal Physiol 302: F713-F721, 2012. First published December 7, 2011 doi:10.1152/ajprenal.00541.2011.-Exocytosis of Weibel-Palade bodies (WPB) represents a distinct response of endothelial cells to stressors, and local release of WPB contents leads to systemic escalation of this response. We synthesized a glycine-(N␣-Et)lysine-proline-arginine (ITF 1697) peptide that has a potential to inhibit exocytosis of WPB and protect microcirculation. Here, we confirmed an inhibitory effect of ITF 1697 using intravital videoimaging and point-tracking of individual organelles. In an in vivo study, mice were implanted with Alzet osmotic pumps (10 g ITF 1697·kg Ϫ1 ·min Ϫ1 at volume of 1 l/h) and subjected to renal ischemia (IRI). IRI resulted in marked renal injury and elevation of serum creatinine in mice treated with a vehicle. In contrast, renal injury and elevation of creatinine were significantly ameliorated in mice subjected to IRI and receiving ITF 1697. ITF 1697 prevented a systemic response to IRI: a significant surge in the levels of eotaxin and IL-8 (KC; both components of WPB), IL-1␣, IL-1␤, and RANTES was all prevented or blunted by the administration of ITF 1697, whereas the levels of an anti-inflammatory, IL-10, and macrophage inflammatory protein-1␣ were upregulated in ITF 1697-treated animals. En face staining of aortic endothelial cells showed that WPB were depleted after 40 -180 min post-IRI, and this was significantly blunted in aortic preparations obtained from mice treated with ITF 1697. WPB exocytosis contributed to IRI-associated mobilization of endothelial progenitor cells and hematopoietic stem cells, and ITF 1697 blunted their mobilization. Unexpectedly, 1 mo after IRI, mice treated with ITF 1697 showed a significantly more pronounced degree of scarring than nontreated animals. In conclusion, 1) application of ITF 1697 inhibits exocytosis of WPB and IRI; 2) the systemic inflammatory response of IRI is in part due to the exocytosis of WPB and its blockade blunts it; and 3) ITF 1697 improves short-term renal function after IRI, but not the long-term fibrotic complications.cytokines; chemokines; mobilization of stem cells; fibrosis WEIBEL-PALADE BODIES (WPB) are rod-shaped organelles (0.2 ϫ 2-3 m) characteristic of endothelial cells and containing an array of proteins, peptides, and cytokines, which can be released emergently on demand. Among these biologically active compounds are von Willebrand factor, P-selectin, IL-8, endothelin-1, angiopoietin-2 (Ang-2), and many others, which contribute to the induction of a proinflammatory milieu on the one hand but also induce mobilization of stem cells on the other. A list of compounds known to induce exocytosis of WPB is long and includes thrombin, histamine, peptido-leukotrienes, complement components, superoxide anion, VEGF, sphingosine-1-p...

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Section: Discussionmentioning

confidence: 99%

Functional consequences of inhibiting exocytosis of Weibel-Palade bodies in acute renal ischemia

Yasuda

Vasko

Hayek

et al. 2012

American Journal of Physiology-Renal Physiology

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“…4 In other studies, other diverse combinations of markers have been used to identify putative precursors in blood independent from CD45 expression such as total CD34 11,[17][18][19]24 As a result of these heterogenous phenotypes, flowcytometric data may be difficult to interpret, not comparable between the different laboratories, and may result in discrepancies. Moreover, most of the suggested precursor phenotypes cited above were not directly tested for true endothelial capacity, neither in vitro nor in vivo.…”

Section: Enumeration Of Circulation Endothelial Precursorsmentioning

confidence: 99%

“…13,14 Therefore, we also included the common leukocyte marker CD45 in our analysis, which marks CD34 ϩ HPC in postnatal life, 15 but is not expressed on primitive embryonic HPC or ECs. 16 Although circulating CD34 ϩ endothelial precursors were reported to be CD45 positive by some 17,18 and negative by others, 19 the suggested CD45 ϩ or CD45…”

mentioning

confidence: 99%

Endothelial Outgrowth Cells Are Not Derived From CD133 ⁺ Cells or CD45 ⁺ Hematopoietic Precursors

Timmermans

Hauwermeiren

Smedt

et al. 2007

ATVB

329

272

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Objective-Two types of endothelial progenitor cells (EPCs), early EPCs and late EPCs (also called endothelial outgrowth cells [EOCs]), were described in vitro previously. In this report, we dissect the phenotype of the precursor(s) that generate these cell types with focus on the markers CD34, CD133, and vascular endothelial growth factor receptor-2 (VEGFR2) that have been used to identify putative circulating endothelial precursors. We also included CD45 in the analysis to assess the relation between CD34 ϩ hematopoietic progenitors (HPC), CD34 ϩ endothelial precursors, and both in vitro generated EPC types. Addressing this issue might lead to a better understanding of the lineage and phenotype of the precursor(s) that give rise to both cell types in vitro and may contribute to a consensus on their flowcytometric enumeration. Methods and Results-Using

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“…To simplify statistical calculations, the only patient with MBG 0 was reported in the MBG-1 group (8 patients). In 66% of the patients (40 patients), a total absence of collateral circulation was observed (Rentrop [20][21][22][23] One of the released factors is hypoxia-induced factor 1, which stimulates the secretion of growth factors such as VEGF and fibroblast growth factor.…”

Section: Angiographic Parameters and Left Ventricular Sys-mentioning

confidence: 99%

Associations between selected angiographic parameters and the number of CD34+ cells and plasma levels of vascular endothelial growth factor and angiogenin in patients with ST-segment elevation myocardial infarction

Grąbczewska¹,

Dębski²,

Góralczyk³

et al. 2015

Polish Archives of Internal Medicine

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Interleukin-8 is associated with circulating CD133+ progenitor cells in acute myocardial infarction

Abstract: In AMI, IL-8 is associated with circulating progenitor cells. In addition to the pro-angiogenic functions of IL-8 and VEGF, this mechanism may contribute to new vessel generation and, thereby, improve myocardial function.

Cited by 108 publications

References 32 publications

Functional consequences of inhibiting exocytosis of Weibel-Palade bodies in acute renal ischemia

Functional consequences of inhibiting exocytosis of Weibel-Palade bodies in acute renal ischemia

Endothelial Outgrowth Cells Are Not Derived From CD133 ⁺ Cells or CD45 ⁺ Hematopoietic Precursors

Associations between selected angiographic parameters and the number of CD34+ cells and plasma levels of vascular endothelial growth factor and angiogenin in patients with ST-segment elevation myocardial infarction

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Interleukin-8 is associated with circulating CD133+ progenitor cells in acute myocardial infarction

Abstract: In AMI, IL-8 is associated with circulating progenitor cells. In addition to the pro-angiogenic functions of IL-8 and VEGF, this mechanism may contribute to new vessel generation and, thereby, improve myocardial function.

Cited by 108 publications

References 32 publications

Functional consequences of inhibiting exocytosis of Weibel-Palade bodies in acute renal ischemia

Functional consequences of inhibiting exocytosis of Weibel-Palade bodies in acute renal ischemia

Endothelial Outgrowth Cells Are Not Derived From CD133 + Cells or CD45 + Hematopoietic Precursors

Associations between selected angiographic parameters and the number of CD34+ cells and plasma levels of vascular endothelial growth factor and angiogenin in patients with ST-segment elevation myocardial infarction

Contact Info

Product

Resources

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Endothelial Outgrowth Cells Are Not Derived From CD133 ⁺ Cells or CD45 ⁺ Hematopoietic Precursors