Interleukin-8 is a mediator of the final cervical ripening in humans

Sennström, Maria; Brauner, Annelie; Lü, Ying; Granström, Lena; Malmström, Anders; Ekman, Gunvor

doi:10.1016/s0301-2115(97)02757-7

Cited by 101 publications

(58 citation statements)

References 17 publications

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“…Many other genes not previously known to be differentially regulated with cervical ripening were also identified. (6) In contrast to cervical dilation after term labor, [12,15] inflammation-related genes did not emerge as differentially expressed with cervical ripening.…”

Section: Commentmentioning

confidence: 88%

“…This procedure has been used extensively by investigators in the United States, Europe and other continents [2,3,6,7,[9][10][11]13,15,16,18,23,[25][26][27][28]31,35,[37][38][39][40]. Half centimeter biopsies were obtained transvaginally from the anterior lip of the cervix at the 12 o'clock position and immediately snap frozen in liquid nitrogen or placed in RNAlater 1 (Ambion, Inc., Austin, TX) and stored at -708C.…”

Section: Methodsmentioning

confidence: 99%

“…premature cervical dilation in the midtrimester) pregnancies. Human cervical biopsy specimens have been obtained from patients in preterm and term labor, preterm and term nonlabor and nonpregnant patients after a hysterectomy to describe the cervical extracellular matrix and its relationship to abnormal labor [2][3][4][5][6][7][8][9][10][11][12][13]15,16,18,25,28,[46][47][48]. Animal studies have also provided insight into the changes in extracellular matrix that occur in the uterine cervix during pregnancy [17,[19][20][21][22]28,33,34,36,[49][50][51][52][53][54].…”

Section: Importance Of the Studymentioning

confidence: 99%

“…The mechanisms of cervical change in pregnancy have been investigated in animals as well as in humans . Critical hypothesis-driven studies of cervical biology have resulted in an improved understanding of the changes that occur in the cervical extracellular matrix during pregnancy and labor and delivery [2,3,5,6,[9][10][11][18][19][20]23,[26][27][28][29][30][31][32][33][34][35][36]. However, the current knowledge of the mechanisms involved in cervical change during pregnancy does not provide a complete understanding of the processes involved in human cervical ripening.…”

Section: Introductionmentioning

confidence: 99%

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The transcriptome of cervical ripening in human pregnancy before the onset of labor at term: Identification of novel molecular functions involved in this process

Hassan¹,

Romero²,

Tarca³

et al. 2009

The Journal of Maternal-Fetal & Neonatal Medicine

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Objective. The aim of this study was to identify changes in the cervical transcriptome in the human uterine cervix as a function of ripening before the onset of labor. Study Design. Human cervical tissue was obtained from women at term not in labor with ripe (n ¼ 11) and unripe (n ¼ 11) cervices and profiled using Affymetrix GeneChip 1 HGU133Plus2.0 arrays. Gene expression was analyzed using a moderated t-test (False Discovery Rate 5%). Gene ontology and pathway analysis were performed. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used for confirmation of selected differentially expressed genes. Results.(1) Ninety-one genes were differentially expressed between ripe and unripe groups. (2) Cervical ripening was associated with enrichment of specific biological processes (e.g. cell adhesion, regulation of anatomical structure), pathways and 11 molecular functions (e.g. extracelluar matrix (ECM)-structural constituent, protein binding, glycosaminoglycan binding). (3) qRT-PCR confirmed that 9 of 11 tested differentially expressed genes (determined by microarray) were upregulated in a ripe cervix (e.g. MYOCD, VCAN, THBS1, COL5A1). (4) Twenty-three additional genes related to ECM metabolism and adhesion molecules were differentially regulated (by qRT-PCR) in ripe cervices. Conclusion.(1) This is the first description of the changes in the human cervical transcriptome with ripening before the onset of labor. (2) Biological processes, pathways and molecular functions were identified with the use of this unbiased approach. (3) In contrast to cervical dilation after term labor, inflammation-related genes did not emerge as differentially regulated with cervical ripening. (4) Myocardin was identified as a novel gene upregulated in human cervical ripening.

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Section: Commentmentioning

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Section: Importance Of the Studymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The transcriptome of cervical ripening in human pregnancy before the onset of labor at term: Identification of novel molecular functions involved in this process

Hassan¹,

Romero²,

Tarca³

et al. 2009

The Journal of Maternal-Fetal & Neonatal Medicine

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show abstract

“…Cervical ripening is thought to be brought about by the interaction of NO and several other inflammatory cytokines which are produced in the cervix [11,12]. NO donors cause the release of NO in vivo.…”

Section: Introductionmentioning

confidence: 99%

The efficacy of two different doses of vaginal isosorbide mononitrate in pre induction cervical ripening: a double blind randomised controlled trial

Vidanagamage

Goonewardene

2011

Ceylon Med. J.

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Objective Nitric oxide donors have been shown to cause cervical ripening. The aim of this study was to evaluate the effects on the mother and the fetus when isosorbide mononitrate (ISMN) 40 mg or the sustained release ISMN (ISMN -SR) 60 mg was administered vaginally for preinduction cervical ripening.Methods A double blind randomised controlled trial. Consecutive women (n=156) with uncomplicated singleton pregnancies between 40 weeks +5 days and 41 weeks' gestation with modified Bishop Scores (MBS) <5 were allocated by stratified (primp / multip) block randomisation to receive ISMN 40 mg, ISMN -SR 60 mg or the placebo vitamin C 100 mg vaginally.Results At the commencement of the study the parity and mean age, MBS, pulse rate (PR), systolic and diastolic blood pressure (SBP and DBP), umbilical artery resistance index (RI) and pulsatility index (PI) were similar among the three treatment groups. There was significant increase (p<0.001) of mean MBS by 1.3 (95% CI 0.8, 1.7) in primips and by 1.7 (95% CI 1.3, 2.0) in multips at 6 hours and by 2.4 (95% CI 1.9, 2.7 ) in primips and by 2.3 (95% CI 2.0, 2.6) in multips at 48 hours. Greater proportions of primips (42% with ISMN -SR 60 mg and 31% with ISMN 40 mg, p<0.05) were favourable for induction of labour (IOL) after 48 hours in comparison with the controls (7.6%). Greater proportions of multips (46% with ISMN -SR 60 mg and 40% with ISMN 40 mg, p<0.05) were favourable for IOL after 48 hours in comparison with the controls (16%). A mean increase of PR by 6.7-10.2 bpm (95% CI 5.0 -12.5, p<0.001) in both ISMN groups at 180 minutes persisted up to 360 minutes. A mean reduction of SBP by 7.3 -10 mmHg (95% CI = 8.0-11.5, p<0.001) in both ISMN groups at 180 minutes persisted up to 360 mins. No significant change was seen in DBP, RI or PI. Frequency of maternal side effects (mainly headache) were higher in ISMN groups. Conclusions

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Expression of adhesion molecules in the lower uterine segment during term and preterm parturition

Winkler

Kemp

Fischer

et al. 2003

Microscopy Res & Technique

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Term and preterm cervical ripening and dilatation have similarities with an inflammatory reaction. Since cell adhesion molecules are involved in this process, investigations on the expression of intercellular adhesion molecule-1, endothelial leukocyte adhesion molecule-1, vascular cell adhesion molecule-1, and platelet endothelial cell adhesion molecule in the lower uterine segment and in vitro experiments on human umbilical vein endothelial cells were performed. In addition, current reports on expression of endothelial adhesion molecules by the uterine cervix were summarized. Cell adhesion molecule expression by lower uterine segment and uterine cervix in term and preterm parturition was measured using immunohistochemistry, enzyme immunoassay, and Northern blot analysis. Regulation of adhesion molecule expression was evaluated in vitro by indirect immunofluorescence and flow cytometry using human umbilical vein endothelial cells. Investigations in term parturition revealed that intercellular adhesion molecule-1, endothelial leukocyte adhesion molecule-1, and vascular cell adhesion molecule-1 expression increases during parturition. In preterm labor, the expression of endothelial leukocyte adhesion molecule-1 and intercellular adhesion molecule-1 in the lower uterine segment increased. Expression of platelet endothelial cell adhesion molecule did not change in term and preterm parturition. Expression of adhesion molecules was localized mainly on lower uterine segment vascular endothelial cells and to a smaller extent on leukocytes. In vitro experiments showed that expression of adhesion molecules by human umbilical vein endothelial cells can be stimulated by tumor necrosis factor-alpha, 17beta-estradiol, prostaglandin E(2), and the antigestagen onapristone. Progesterone exerted no stimulatory effect. Cervical ripening and dilatation during term and preterm parturition are associated with an increased expression of endothelial cell adhesion molecules by lower uterine segment and uterine cervix. The expression can be modulated by pro-inflammatory cytokines, sex hormones, and prostaglandin E(2). Mechanisms controlling the extravasation of leukocytes may play a fundamental role in term and preterm parturition.

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Interleukin-8 is a mediator of the final cervical ripening in humans

Cited by 101 publications

References 17 publications

The transcriptome of cervical ripening in human pregnancy before the onset of labor at term: Identification of novel molecular functions involved in this process

The transcriptome of cervical ripening in human pregnancy before the onset of labor at term: Identification of novel molecular functions involved in this process

The efficacy of two different doses of vaginal isosorbide mononitrate in pre induction cervical ripening: a double blind randomised controlled trial

Expression of adhesion molecules in the lower uterine segment during term and preterm parturition

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