Interleukin-8 in sepsis: relation to shock and inflammatory mediators

Abstract

Because of its neutrophil-activating properties, interleukin-8 (IL-8) may play an important role in the pathophysiology of sepsis. We measured circulating IL-8 levels in 47 patients with clinical sepsis. Levels on admission were elevated in 42 of the 47 patients (89%o) and were comparable in patients with gram-positive or gram-negative infections. Patients with shock had significantly higher IL-8 levels than normotensive patients (P = 0.0014, Wilcoxon-Mann-Whitney test), whereas no differences in IL-8 levels were found between patients with or without adult respiratory distress syndrome. Patients who died had higher IL-8 levels on admission than the patients who survived. The largest differences in IL-8 levels between survivors and nonsurvivors was found when only patients with positive cultures were considered (P = 0.0342). IL-8 levels appeared to correlate significantly with lactate levels and inversely with leukocyte and platelet numbers and mean arterial pressure. In addition, the IL-8 level in the sepsis patients was found to correlate significantly with levels of IL-6, elastase-al-antitrypsin, and C3a. Serial observations revealed that in most patients IL-8 levels decreased, irrespective of the outcome. Thus, our results demonstrate that IL-8 levels are increased in most patients with sepsis and correlate with some important clinical, biochemical, and inflammatory parameters. These findings suggest a role for IL-8 in the pathophysiology of sepsis.INFECT. IMMUN.

Interleukin 10 Release During Endotoxaemia in Chimpanzees: Role of Platelet‐Activating Factor and Interleukin 6

“…Serum was obtained by centrifugation at 4 C for 20 min at 1600 g, and stored at biotinylated anti-IL-10 MoAb JES3-12G8 (Pharmingen) as detecting antibody, and recombinant human IL-10 (Medgenix, Fleurus, Belgium) as standard, exactly as described previously [9]. IL-6 and IL-8 were measured by ELISA [15,16].…”

Circulating concentrations of soluble granzyme A and B increase during natural and experimental Plasmodium falciparum infections

“…Minimum detection level for sGranzyme A was 3 pg/ml and for sGranzyme B 2 pg/ml. IL-8 [18], IL-10 [19], IL-12p40, IL-12p70, IFN g [20] and CRP [21] were also measured by ELISA, accordingly the publication procedure.…”

Human intravenous immunoglobulin (IVIG) preparations degranulate human neutrophilsin vitro

“…CLB-FcR/gran-1 (5D2, IgG2a [16]) was produced in our own institute. The MoAbs against tumour necrosis factor-alpha (TNF-a) (aTNF/5, aTNF/7 [17]) and IL-8 (aIL-8/1 [18]) have been described before. F(ab) fragments were made by digestion with 4% (w/w) papain for 1·5 h at 37ЊC in PBS containing 10 mM cysteine and 5 mM EDTA.…”