Interleukin-8 associates with adhesion, migration, invasion and chemosensitivity of human gastric cancer cells

Kuai, Wenxia; Wang, Qiong; Yang, Xiaozhong; Zhao, Yao; Ren, Yu; Tang, Xiaojun

doi:10.3748/wjg.v18.i9.979

Cited by 103 publications

(90 citation statements)

References 20 publications

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“…In a previous study, using treatment of IL-8 at a concentration of >1 ng/ml, we reported that IL-8 could enhance the adhesion, migration and invasion of the human gastric cancer SGC-7901 cell line in a dose-dependent manner (16). This result was supported by another similar study by Kuai et al using cDNA and small interfering RNA transfectants (35). However, IL-8 production by gastric cells is marginal, at even less than 1 ng/ml.…”

supporting

confidence: 76%

Low-dose interleukin-8 induces the adhesion, migration and invasion of the gastric cancer SGC-7901 cell line

Shi

Wei

2015

Oncology Letters

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Abstract. , an important inflammatory cytokine, is strongly associated with gastric cancer development and metastasis. High-dose (>1 ng/ml) IL-8 has been revealed to promote the adhesion, migration and invasion of human gastric cancer SGC-7901 cells in a dose-dependent manner. However, the IL-8 level produced by gastric cells is marginal, at even less than 1 ng/ml. It is unclear whether low-dose IL-8 also induces these capacities. In the present study, the effect of low-dose IL-8 on the adhesion, migration and invasion of the SGC-7901 cell line and the underlying molecular mechanism with regard to cluster of differentiation 44 (CD44) were investigated. The SGC-7901 cells were exposed to various concentrations of IL-8 (0, 0.2, 0.5, 0.8 and 1 ng/ml) in vitro. The adhesion of the SGC-7901 cells to fibronectin, an extracellular matrix component, was then detected by cell counting kit 8 assay. Migration and invasion abilities were evaluated by wound scratch and Transwell chamber assays. In addition, protein and mRNA levels of CD44 were measured using immunofluorescence and western blotting, and quantitative polymerase chain reaction, respectively, in cells cultured for 72 h. Following the exposure of the SGC-7901 cells to the various low doses of IL-8, the cell adhesion, migration and invasion capacities were promoted by IL-8, but not in a significant dose-dependent manner. Low-dose IL-8 upregulated the protein and mRNA expression of CD44. In conclusion, low-dose IL-8 potently induces the adhesion, migration and invasion of SGC-7901 cells, and the regulation of CD44 expression is one of the potential molecular mechanisms involved.

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supporting

confidence: 76%

Low-dose interleukin-8 induces the adhesion, migration and invasion of the gastric cancer SGC-7901 cell line

Shi

Wei

2015

Oncology Letters

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“…In vivo, IL-8 is important in the depth of invasion and venous and lymphatic invasion of tumors, and may be an independent prognostic factor in human gastric carcinoma (35). In vitro, the IL-8 level is significantly associated with the adhesion, migration, invasion and chemosensitivity of human gastric cancer cells (36,37).…”

Section: Discussionmentioning

confidence: 99%

Interleukin-8: A potent promoter of angiogenesis in gastric cancer

Shi

Wei

2015

Oncology Letters

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“…Chemotherapeutic agents are known to induce IL-8 upregulation in tumor cells, which is closely associated with chemotherapy resistance and cancer metastasis (3,(5)(6)(7)10,18,(21)(22)(23)(24)(25)(37)(38)(39)(40). Depletion of IL-8 induces cell cycle arrest and increases the efficacy of chemotherapeutic agents in breast cancer cells (41).…”

Section: Discussionmentioning

confidence: 99%

“…Metastasis after resection, is the leading cause of mortality in patients with breast cancer (1,2). Interleukin-8 (IL-8), a cytokine of the CXC chemokine family (3), is highly expressed in many tumor tissues (4) and promotes tumor progression and cancer metastasis (5)(6)(7)(8)(9)(10). Findings of recent studies have shown that the overexpression of IL-8 is associated with recurrence and poor prognosis in breast cancer (11)(12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

Berberine hydrochloride IL-8 dependently inhibits invasion and IL-8-independently promotes cell apoptosis in MDA-MB-231 cells

Zhao

Shi

et al. 2014

Oncology Reports

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Abstract. Breast cancer, the leading cause of cancer-related mortality worldwide in females, has high metastastic and recurrence rates. The aim of the present study was to evaluate the anti-metastatic and anticancer in situ effect of berberine hydrochloride (BER) in MDA-MB-231 cells. BER dose-dependently inhibited proliferation and the IL-8 secretion of MDA-MB-231 cells. Additional experiments revealed that the inactivation of PI3K, JAK2, NF-κB and AP-1 by BER contributed to the decreased IL-8 secretion. BER abrogated cell invasion induced by IL-8 accompanied with the downregulation of the gene expression of MMP-2, EGF, E-cadherin, bFGF and fibronectin.In addition, BER reduced cell motility but induced G2/M arrest and cell apoptosis in an IL-8-independent manner. BER modulated multiple signaling pathway molecules involved in the regulation of cell apoptosis, including activation of p38 MAPK and JNK and deactivation of JAK2, p85 PI3K, Akt and NF-κB. The enhanced cell apoptosis induced by BER was eliminated by inhibitors of p38 MAPK and JNK but was strengthened by activator of p38 MAPK. Thus, BER inhibited cell metastasis partly through the IL-8 mediated pathway while it induced G2/M arrest and promoted cell apoptosis through the IL-8 independent pathway. Apoptosis induced by BER was mediated by crosstalks of various pathways including activation of p38 MAPK and JNK pathways and inactivation of JAK2/ PI3K/NF-κB/AP-1 pathways. The results suggested that BER may be an efficient and safe drug candidate for treating highly metastatic breast cancer.

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Interleukin-8 associates with adhesion, migration, invasion and chemosensitivity of human gastric cancer cells

Abstract: Overexpression of IL-8 promotes the adhesion, migration, invasion, and chemoresistance of GC cells, indicating that IL-8 is an important therapeutic target in GC.

Cited by 103 publications

References 20 publications

Low-dose interleukin-8 induces the adhesion, migration and invasion of the gastric cancer SGC-7901 cell line

Low-dose interleukin-8 induces the adhesion, migration and invasion of the gastric cancer SGC-7901 cell line

Interleukin-8: A potent promoter of angiogenesis in gastric cancer

Berberine hydrochloride IL-8 dependently inhibits invasion and IL-8-independently promotes cell apoptosis in MDA-MB-231 cells

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