Interleukin-7 induces the proliferation of normal human B-cell precursors

Saeland, Sem; Duvert, V; Pandrau, D.; Caux, Christophe; Durand, Isabelle; Wrighton, Nicholas C.; Wideman, Janusz; Lee, Frank; Banchereau, Jacques

doi:10.1182/blood.v78.9.2229.2229

Cited by 68 publications

(18 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IL-3 and IL-4 are the most potent cytokines, enhancing, together with CD40 crosslinking, activation and proliferation of normal B-cell precursors (Saeland et al, 1993;Larson & LeBien, 1994) and mature B cells , respectively. Although IL-7 promotes the growth of normal B-cell precursors (Saeland et al, 1991), the effect of IL-7 on blast cell proliferation in ALL is controversial (Eder et al, 1990;Makrynikola et al, 1991;Takeda et al, 1989). We demonstrated that activation of the CD40 antigen in the presence of IL-3 prevented cell death, and even induced proliferation of most precursor B-lineage ALLs, but that IL-7 was not active in these conditions.…”

mentioning

confidence: 63%

Proliferation of precursor B‐lineage acute lymphoblastic leukaemia by activating the CD40 antigen

Planken¹,

Dijkstra²,

Bâkkus³

et al. 1996

Br J Haematol

View full text Add to dashboard Cite

No reliable culture system exists for B-lineage acute lymphoblastic leukaemia (ALL). Recently we found that many different mature B-cell malignancies proliferate upon stimulation via the CD40 antigen, and this led us to investigate whether a similar CD40 activation on ALL cells could also induce proliferation. First, we measured CD40 expression in 21 ALL cases; all were CD40+, although mostly weak. Next, we triggered the CD40 antigen by anti-CD40 antibodies and by a CD40 ligand-expressing cell line. In addition, we measured the influence of IL-3, IL-4 and IL-7 with and without these stimuli. In 8/10 cases proliferation, measured by 3H-thymidine incorporation, could be induced after CD40 crosslinking, especially in the presence of IL-3. Stimulation via the CD40 ligand was more successful than using crosslinked anti-CD40 antibodies. IL-4 inhibited the spontaneous proliferation found in three cases, but stimulated proliferation after CD40 crosslinking. IL-7 did not contribute to proliferation. Morphology, immunophenotyping and surface marker analysis, combined with DNA flow cytometry confirmed that the proliferation found could be ascribed to the ALL cells. In conclusion, B-lineage ALL cases are CD40+, and many can be cultured using CD40 stimulation and IL-3.

show abstract

mentioning

confidence: 63%

Proliferation of precursor B‐lineage acute lymphoblastic leukaemia by activating the CD40 antigen

Planken¹,

Dijkstra²,

Bâkkus³

et al. 1996

Br J Haematol

View full text Add to dashboard Cite

show abstract

“…Either in mice or humans, various cytokines have been suggested to play some role in precursor B cell growth or differentiation. These include IL-2 [21], IL-3 [22], IL-4 [23] and IL-7 [24-271. There is a body of evidence supporting the essential role of IL-7 in promoting mouse precursor B cell growth [28-301. Although human B cell progenitors express the high affinity IL-7R [31], IL-7 was found to have a less crucial effect on human pro-B and pre-B cell growth [27,32]. Recently, this cytokine was shown to down-modulate RAG and TdT-genes and to up-regulate surface CD19 expression in normal human pre-B cells [33].…”

Section: Discussionmentioning

confidence: 99%

IL-7 sensitizes human pre-B cells but not pro-B cells to Fas/APO-1 (CD95)-mediated apoptosis

Lévy¹,

Benlagha²,

Buzyn³

et al. 1997

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYHomeostasis of human B cell development is maintained by a complex network of cytoplasmic and surface expressed molecules. Abnormalities in this process may result in the expansion of malignant B cell precursors in B lineage acute lymphoblastic leukaemia (ALL). ALL cells share surface antigens with normal early precursor B cells. We have studied here the role of Fas/APO-1 (CD95) antigen on leukaemic precursor B cell line growth and survival, and the modulation of its effects by signals involved in normal early B cell development. Four ALL cell lines representative of the early steps of B cell differentiation are shown to express surface Fas/APO-1 (CD95) antigen and to undergo apoptosis in the presence of anti-Fas cross-linking antibodies. This effect is strongly enhanced when pre-B, but not pro-B cells, are pretreated with IL-7 but not with IL-2, IL-3, IL-4 or IL-10. Furthermore, pre-B cell death induced by anti-Fas antibodies in combination with IL-7 is increased upon pre-B receptor but not CD19 cross-linking. Bcl-2 and Bax protein expression is not influenced by IL-7 or pre-BR stimulation in either pro-B or pre-B cell lines. These results indicate that signals involved in normal early B cell development can modulate the Fas (CD95)-mediated apoptosis of leukaemic precursor B cells.

show abstract

“…IL-7 (provided by Dr. E Lee, DNAX) was used as a 5 % dilution of a culture supernatant of COS 7 cells (approximately 15 U/ml) transfected with the human I C 7 cDNA clone. This concentration was found to induce maximum proliferation of early human B cell precursors [21].…”

Section: Factorsmentioning

confidence: 95%

Proliferation and differentiation of human CD5⁺ and CD5⁻ B cell subsets activated through their antigen receptors or CD40 antigens

Defrance¹,

Vanbervliet²,

Durand³

et al. 1992

Eur J Immunol

View full text Add to dashboard Cite

The pan-T cell antigen CD5 has been shown to delineate two different mouse B cell subsets, originating from distinct progenitors. In man, on average, 30% of the tonsillar B cell pool expresses this antigen. In the present report, a detailed comparison of the CD5+ and CD5- B cell response to cytokines, following activation via surface immunoglobulins (sIg) or CD40 antigen, was undertaken. CD5+ B cells were positively selected by panning or by sorting from tonsils. Two-color immunofluorescence analysis performed on tonsillar B cell populations showed that CD5+ B cells displayed most of the phenotypic features of mantle zone B cells. CD5+ B cells could be stimulated for DNA synthesis by mitogenic concentrations of Staphylococcus aureus, Cowan I strain (SAC), insolubilized anti-IgM antibodies, immobilized anti-CD40 antibodies and phorbol 12-myristate 13-acetate (PMA). The growth-response of small dense CD5- B cells to these T cell-independent mitogens was comparable to that of CD5+ B cells, whereas the low-density, in vivo-activated, CD5- B cells were only marginally stimulated by Ig-cross-linking agents and PMA. Following ligation of sIg, both B cell subsets proliferated essentially in response to interleukin (IL)-2 and IL-4. When used in co-stimulation with immobilized anti-CD40 antibodies, IL-4 promoted growth of CD5+ and CD5- B cells, whereas IL-2 displayed only moderate stimulatory effects. CD5+ and CD5- B cells differentiated into Ig-secreting cells when they were co-cultured with SAC or cross-linked anti-CD40 antibodies and IL-2. However, IgM constituted the major component of the Ig response of CD5+ B cells, whereas high levels of IgG were secreted by CD5- B cells.

show abstract

Interleukin-7 induces the proliferation of normal human B-cell precursors

Cited by 68 publications

References 29 publications

Proliferation of precursor B‐lineage acute lymphoblastic leukaemia by activating the CD40 antigen

Proliferation of precursor B‐lineage acute lymphoblastic leukaemia by activating the CD40 antigen

IL-7 sensitizes human pre-B cells but not pro-B cells to Fas/APO-1 (CD95)-mediated apoptosis

Proliferation and differentiation of human CD5⁺ and CD5⁻ B cell subsets activated through their antigen receptors or CD40 antigens

Contact Info

Product

Resources

About

Interleukin-7 induces the proliferation of normal human B-cell precursors

Cited by 68 publications

References 29 publications

Proliferation of precursor B‐lineage acute lymphoblastic leukaemia by activating the CD40 antigen

Proliferation of precursor B‐lineage acute lymphoblastic leukaemia by activating the CD40 antigen

IL-7 sensitizes human pre-B cells but not pro-B cells to Fas/APO-1 (CD95)-mediated apoptosis

Proliferation and differentiation of human CD5+ and CD5− B cell subsets activated through their antigen receptors or CD40 antigens

Contact Info

Product

Resources

About

Proliferation and differentiation of human CD5⁺ and CD5⁻ B cell subsets activated through their antigen receptors or CD40 antigens