Interleukin‐6 regulates expression of Fos and Jun genes to affect the development of mouse preimplantation embryos

Abstract

AimWe investigated whether recombinant mouse interleukin‐6 (IL‐6) affects the development of preimplantation embryos and induces the ‐signal transducers and activators of transcription (JAK–STAT) signaling pathway by binding IL‐6 signal transducer (IL‐6st) and regulating Fos and Jun gene expression, thereby accounting for the negative effect of superovulation on embryo development.MethodsWe compared rates of blastocyst formation from embryos cultured with different concentrations of IL‐6 or/and anti‐interleukin 6 receptor antibody (anti‐IL‐6RAb) in superovulated experimental and normal control groups. IL‐6 expression in preimplantation embryos was determined by immunofluorescence identification. Fos, Jun and IL‐6st messenger RNA expression was detected by PCR and microarray experiments.ResultsRates of blastocyst formation significantly decreased in superovulated embryos, whether or not they were incubated in 0.1, 1, 25 or 50 pg/mL IL‐6, (P < 0.01) compared to embryos from naturally ovulated controls, whereas incubation in 5 and 10 pg/mL IL‐6 reversed the negative effects of superovulation. The addition of anti‐IL‐6RAb to naturally ovulated embryos reduced blastocyst rates to those of superovulated embryos. Gene chip analysis indicated that the JAK–STAT signaling pathway contained differentially expressed IL ‐6, IL ‐6st, Jun and Fos genes. Both anti‐IL‐6RAb or ovarian stimulation downregulated IL‐6st, Jun, and Fos messenger RNA expression, but expression of the same three genes increased in 5 pg/mL IL‐6.ConclusionOvarian stimulation negatively impacts the development of preimplantation embryos by reducing IL‐6 release. IL‐6 affects the rate of development of zygotes to blastocyst by regulating IL ‐6st, F os and J un expression in the JAK–STAT signaling pathway.

Cyr61 synthesis is induced by interleukin-6 and promotes migration and invasion of fibroblast-like synoviocytes in rheumatoid arthritis

“…Based on our DEG results, we selected candidates such as c-Jun, NR4A1, ATF3, and EGR3 to determine whether they regulated IL-6-induced Cyr61 protein synthesis. Previous studies have suggested that the effect of IL-6 on target gene transcription may involve c-Jun [ 26 ]. Hence, we examined whether c-Jun was involved in the IL-6-induced increase in Cyr61 protein synthesis and found that knocking down c-Jun expression did not affect IL-6-induced Cyr61 protein synthesis.…”

Effects of quercetin on Aroclor 1254-induced expression of CYP450 and cytokines in pregnant rats

“…Since many of these same agents are also released into the general circulation, distal toxicities may also manifest and could harm an embryo/fetus (Mori et al 2016). IL-6 is also functionally associated with embryonic development; elevated IL-6 concentrations were associated with a decline in blastocyst formation rates (Yu et al 2018). Here, hepatic/serum levels of IFNc, IL-2, and IL-6 in Aroclor 1254-treated rats were significantly increased and there were significant increases in host IFNc/IL-4 (T H 1/T H 2) ratios, suggesting that the immune balance of T H 1/T H 2 moves toward T H 1.…”

CYR61 synthesis is Induced by Interleukin-6 and Promotes Migration and Invasion of Fibroblast-like Synoviocytes in Rheumatoid Arthritis

“…Based on our DEG results, we selected candidates such as c-jun, NR4A1, ATF3, and EGR3 to determine whether they regulated IL-6-induced Cyr61 protein synthesis. Previous studies have suggested that the effect of IL-6 on target gene transcription may involve c-jun [26]. Hence, we examined whether c-jun was involved in the IL-6-induced increase in Cyr61 protein synthesis and found that knocking down JUN expression did not affect IL-6-induced Cyr61 protein synthesis; NR4A1 and ATF3 also did not show any signi cant effects.…”