Interleukin-6 is a key mediator of the hepatoprotective and pro-proliferative effects of ischaemic preconditioning in mice

Teoh, Narci C.; Field, Jacqueline; Farrell, Geoffrey C.

doi:10.1016/j.jhep.2006.01.039

Cited by 74 publications

(71 citation statements)

References 42 publications

(73 reference statements)

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“…It also has promotes liver regeneration after liver injury. 31 Like IL-6, IL-10 can also inhibit TNF-a and IL-1b production by macrophages and peripheral monocytes, at least in part by downregulating the transcription of TNF-a mRNA in response to LPS. 32 The competing expression of pro-and anti-inflammatory cytokines may determine the severity of inflammation, tissue damage and mortality.…”

Section: Determination Of Neutrophil Influx Into the Liver Bymentioning

confidence: 99%

Immune response and pathophysiological features of Klebsiella pneumoniae liver abscesses in an animal model

Fung

Chang

Lin

et al. 2011

Laboratory Investigation

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Capsular serotypes K1 and K2, the rmpA gene (a regulator of the mucoid phenotype) and aerobactin from Klebsiella pneumoniae have been identified as the major virulence factors for pyogenic liver abscesses with high morbidity, mortality and severe complications. The pathological mechanisms remain unclear. In this study, we compared liver immune responses and pathological changes in response to different serotypes of K. pneumoniae infections. A mouse model was used to investigate cytokine and chemokine production, histopathology findings, phagocytic uptake and mortality induced by serotypespneumoniae serotypes K1 and K2 showed lower 50% lethal dose values and more phagocytic resistance to neutrophils than K62 and the DK1 mutant. In sequential liver samples, viable bacteria counts increased 3 h to 3 days after low-dose inoculation (o10 1 colony-forming unit (cfu)) with K1 and K2, while K62 and DK1 cleared rapidly and became undetectable even with high-dose inoculation (B2.9 Â 10 5 cfu). Time-dependent increases in cytokines and chemokines, including tumor necrosis factor-a, interleukin (IL)-1b, IL-6, IL-10, keratinocyte-derived chemokines and macrophage inflammatory protein-2, were observed in the serum and liver tissue of K1-and K2-infected mice, and severe disease progression manifesting as microabscesses was also identified. K62 and DK1 inoculation did not result in similar immune responses and histological changes. These findings illustrate the critical role of phagocytic resistance against innate immunological defense mechanisms as well as its contribution to the development of liver abscesses.

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Section: Determination Of Neutrophil Influx Into the Liver Bymentioning

confidence: 99%

Immune response and pathophysiological features of Klebsiella pneumoniae liver abscesses in an animal model

Fung

Chang

Lin

et al. 2011

Laboratory Investigation

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“…IL-6 and IL-10 have the potential to inhibit TNF-α and IL-β expression from macrophage and peripheral monocytes [27,28]. IL-6 also has the anti-inflammatory potential by activation of transcription-3 protein in hepatocytes [27].…”

Section: Discussionmentioning

confidence: 99%

Contribution of Outer Membrane Porin (Omp) K36 to Klebsiella pneumoniae induced Liver Abscess

Chang¹,

Tsai²,

Chen³

2016

Int J Clin Med Microbiol

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Introduction: Pyogenic liver abscess is usually caused by multiple bacterial infections. This disease can be caused by single microorganism, Klebsiella pneumoniae that has been reported in Asia, North America and Europe. Many virulence factors of K. pneumoniae have identified including capsular polysaccharide, lipopolysaccharide, serum resistance, adhesins and siderophores. Previously, we demonstrated the virulent effect of outer membrane porin (OMP) K 36 by a lethality study. However, the role of OmpK36 in liver abscess pathogenesis is still unclear.Objective: In this study, a virulent K. pneumoniae strain NVT-1002 and OmpK36 deficient mutant (ΔOMPK) were used to examine the contribution of OmpK36 to liver abscess. Liver injuries and inflammatory cytokines expression were detected by tissue section and enzyme linked-immunosorbent assay respectively. The toxic effect of OmpK36 to human hepatoma cell (HepG2) was tested by bacterial infections and the treatment with OmpK36 recombinant protein. Results:The Results demonstrated that the NVT-1002 induced sever liver injuries while little or no damage was found in mice injected with OmpK36. Expression of serum and liver cytokines including TNF-α, IL-1β, IL-6 and IL-10 were elevated after injection of NVT-1002, meanwhile, only IL-1β was transiently elevated in OmpK36 group. Interestingly, OmpK36 recombinant protein showed no toxic effect to HepG2 cells, and the virulence of OmpK36 mutant to HepG2 was not altered compared to NVT-1002 group. Conclusion:In conclusion, OmpK36 contributes to the K. pneumoniae induced liver abscess and inflammatory responses. The virulent effect of OmpK36 is not mediated by the protein itself, but other uncertain mechanisms. OmpK36 may be a therapeutic target in K. pneumoniae infection. Contribution of Outer Keywords:OmpK36, Virulence, Liver abscess, Inflammatory Original ArticleOpen Access IntroductionKlebsiella pneumoniae have been documented as the common factor for cryptogenic liver abscess in Asia Pacific [1,2]. Similar findings in North America and Europe have also been reported [3][4][5]. Several virulent factors of K. pneumoniae were discovered and these factors are almost related to specific capsular polysaccharides (CPS) [2,[6][7][8]. Accumulating reports have indicated that the capsule is essential to the virulence of Klebsiella species [9][10][11]. Up to now, the capsular antigen have been classified into 77 serotypes , and serotype K1 and K2 capsular antigen were found to comprise virulence in mouse peritonitis model, whereas isolates of serotypes other than K1 or K2 were with little or no virulence [5,12].Outer membrane porins (OMP) are proteins that cross a cellular membrane and contribute to the diffusion of molecules. Many studies have showed OMPs play important roles in antimicrobial resistance [13,14]. Previously, we have demonstrated the virulent effects of OMPK 36 by an animal study [15]. In this report, the results of animal lethality study showed that the virulence of OmpK36 mutant was reduced about 100 fold c...

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“…6 piglets, the amount of applied stem cells: 8. 75, 14.0, 17.0, 17.5, 43.0 and 61.0 x 10 6 MSC respectively) or physiological solution (9 piglets from control group) were applied into non-occluded portal vein branches , Teoh 2006.…”

Section: Surgical Proceduresmentioning

confidence: 99%

“…The selected concentration of applied cytokine initiated acceleration of regeneration of liver parenchyma in non-occluded liver lobes (Heinrich, 2006, Teoh, 2006.…”

Section: Functional Liver Remnant Volumementioning

confidence: 99%

Liver Parenchyma Regeneration in Connection with Extended Surgical Procedure - Experiment on Large Animal

Liška¹,

Třeška²,

Mírka³

et al. 2012

Liver Regeneration

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Interleukin-6 is a key mediator of the hepatoprotective and pro-proliferative effects of ischaemic preconditioning in mice

Cited by 74 publications

References 42 publications

Immune response and pathophysiological features of Klebsiella pneumoniae liver abscesses in an animal model

Immune response and pathophysiological features of Klebsiella pneumoniae liver abscesses in an animal model

Contribution of Outer Membrane Porin (Omp) K36 to Klebsiella pneumoniae induced Liver Abscess

Liver Parenchyma Regeneration in Connection with Extended Surgical Procedure - Experiment on Large Animal

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