Interleukin-6 inhibits adrenal androgen release from bovine adrenal zona reticularis cells by inhibiting the expression of steroidogenic proteins

McIlmoil, S.; Call, Gerald B.; Barney, Michelle; Strickland, J.; Judd, Allan M.

doi:10.1016/j.domaniend.2015.05.006

Cited by 4 publications

(7 citation statements)

References 44 publications

(128 reference statements)

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“…These observations support the hypothesis that ACTH is not a primary secretagogue for DHEA, and that other factors may have a significant role in the regulation of DHEA and DHEAS secretion (23,24,89). It is possible that adrenal DHEA synthesis requires the presence of ACTH, but the basal ACTH-dependent output could be either enhanced or inhibited by other factors.…”

Section: Dhea/dheas Release In Response To Acth and Stressorssupporting

confidence: 79%

“…In some of those studies (21-23), however, androgen concentrations were measured by a commercial adrenal androgen radioimmunoassay that employed an antibody that reacts with all adrenal androgens (DHEA, DHEAS, and androstenedione). Therefore, it is not easy to work out the contribution of DHEA and DHEAS to the androgen pool (20)(21)(22)(23). Interestingly, ACTH differently affects steroidogenic enzymes in the human and bovine adrenals.…”

Section: Dhea/dheas Release In Response To Acth and Stressorsmentioning

confidence: 99%

“…The effects of IL6 in the bovine adrenals are quite complex, as this cytokine inhibits basal and ACTH-stimulated androgen release from the zona reticularis in a dose and time dependent manner; while increased basal and ACTH-stimulated androgen release from mixed adrenocortical cells. In the zona reticularis, IL6 decreased the expression of StAR protein, cholesterol side chain cleavage (CYP11A1) enzyme, CYP17A, HSD3B2, nuclear factor steroidogenic factor 1 (SF-1), that stimulates steroidogenesis, and enhanced the expression of DAX-1, a nuclear factor that inhibits steroidogenesis (23).…”

Section: The Role Of the Immune Systemmentioning

confidence: 99%

“…In humans, adrenal androgen secretion is responsive to ACTH and stressors (10). In domestic mammals, adrenal secretion of DHEA in response to ACTH is well-documented "in vitro" (19)(20)(21)(22)(23). Nevertheless, published "in vivo" studies that have explored DHEA and DHEAS release in response to the administration of exogenous ACTH or stressors produced contradictory results [cattle: (6,24,25); dog: (26,27)].…”

Section: Introductionmentioning

confidence: 99%

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Do Dehydroepiandrosterone (DHEA) and Its Sulfate (DHEAS) Play a Role in the Stress Response in Domestic Animals?

et al. 2020

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In animal husbandry, stress is often associated with poor health and welfare. Stress occurs when a physiological control system detects a state of real or presumptive threat to the animal's homeostasis or a failure to control a fitness-critical variable. The definition of stress has mostly relied on glucocorticoids measurement, even though glucocorticoids represent one stress-response system, the hypothalamus-pituitary-adrenocortical axis, which is not precise enough as it is also related to metabolic regulation and activated in non-stressful situations (pleasure, excitement, and arousal). The mammal adrenal can synthesize the androgenic steroid dehydroepiandrosterone (DHEA) and its sulfate metabolite (DHEAS), which have been associated to the stress response in several studies performed mostly in humans and laboratory animals. Although the functions of these steroids are not fully understood, available data suggest their antagonistic effects on glucocorticoids and, in humans, their secretion is affected by stress. This review explores the scientific literature on DHEA and DHEAS release in domestic animals in response to stressors of different nature (inflammatory, physical, or social) and duration, and the extra-adrenal contribution to circulating DHEA. Then, the potential use of DHEA in conjunction with cortisol to improve the definition of the stress phenotype in farmed animals is discussed. Although the focus of this review is on farmed animals, examples from other species are reported when available.

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Section: Dhea/dheas Release In Response To Acth and Stressorssupporting

confidence: 79%

Section: Dhea/dheas Release In Response To Acth and Stressorsmentioning

confidence: 99%

Section: The Role Of the Immune Systemmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Do Dehydroepiandrosterone (DHEA) and Its Sulfate (DHEAS) Play a Role in the Stress Response in Domestic Animals?

et al. 2020

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“…McIlmoil, Call, Barney, Strickland, & Judd, 2015). Conversely, IL-6 increases dehydroepiandrosterone (DHEA) secretion from human Xian Wei and Junfeng Zhang contributed equally to this study.…”

Section: Introductionmentioning

confidence: 99%

Interleukin‐6 increases adrenal androgen release by regulating the expression of steroidogenic proteins in NCI‐H295R cells

Xian

Zhang

Peng

et al. 2020

Journal Cellular Physiology

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The purpose of this study was to investigate the potential mechanism of interleukin‐6 (IL‐6) on the stimulation of excessive androgen secretion in human NCI‐H295R adrenocortical cells. We performed transcriptome sequencing of cancer and paracancerous tissues obtained from functional adrenal cortical adenomas. The secretion of dehydroepiandrosterone sulfate (DHEAS) in NCI‐H295R cells was detected by a chemiluminescence assay. The expression of messenger RNA (mRNA) was detected by real‐time polymerase chain reaction and that of protein was detected by western blotting. The expression of secretogranin II (SCG2) and IL‐6 were significantly increased in cancer tissues. Upregulation of mRNA and protein levels of AKR1C3, CYP11A, CYP17A1, 3βHSD, and SULT2A1 was observed after stimulation with IL‐6. IL‐6 could also increase the expression of StAR mRNA and proteins. Our results suggest that IL‐6 can promote androgen secretion by regulating the expression of genes related to androgen pathways.

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Interleukin 6 increases the in vitro expression of key proteins associated with steroidogenesis in the bovine adrenal zona fasciculata

McIlmoil

Strickland

Judd

2016

Domestic Animal Endocrinology

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Interleukin-6 inhibits adrenal androgen release from bovine adrenal zona reticularis cells by inhibiting the expression of steroidogenic proteins

Cited by 4 publications

References 44 publications

Do Dehydroepiandrosterone (DHEA) and Its Sulfate (DHEAS) Play a Role in the Stress Response in Domestic Animals?

Do Dehydroepiandrosterone (DHEA) and Its Sulfate (DHEAS) Play a Role in the Stress Response in Domestic Animals?

Interleukin‐6 increases adrenal androgen release by regulating the expression of steroidogenic proteins in NCI‐H295R cells

Interleukin 6 increases the in vitro expression of key proteins associated with steroidogenesis in the bovine adrenal zona fasciculata

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